Lipids of the cottonseed. III. Perchloric acid ashing of lipids for the determination of phosphorus

Alterations in lipid constituents of lamb liver in response to varying doses (O-2.0 Mrad) of gamma-radiation were ascertained in terms of total vitamin A, total phospholipid, and fatty acid content. Radiation exposure at 2.0 Mrad dose resulted in a five-fold increase in thiobarbituric acid reacting substances (TBRS) measured in terms of malonaldehyde and a 64% rise in carbonyls. The losses in lipid constituents were marginal. During the course of autolysis significant lipolytic activities persisted in irradiated lamb liver as evidenced by the decline in total phospholipid (50%) and neutral fat levels accompanied by a four-fold increase in fatty acid content. In irradiated lamb liver undergoing autolysis, a rapid hydrolysis of vitamin A Esters occurred leading to enhanced vitamin A alcohol/ester ratios. A comparison of radiation destruction of vitamin A in model systems, shark liver oil, and lamb liver revealed that similar doses of gamma-radiation cause more destruction in model systems than in others.

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Fish Protein Concentrate (FPC) from Bombay Duck Isolated by Radiation‐Heat Combination Procedure: Functional and Nutritional Properties

Warrier

Ninjoor

1981

Journal of Food Science

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A process involving gamma-irradiation (200 Krad) and heat treatment (60°C, 10 mhl), for the preparation of fish protein concentrate (FPC) from a I ropical fish Bombay duck (Hurpodon nehereus) is described. The procedure enabled the precipitation of 75% of proteins from Bomllay duck muscle which accounted for 80% of myofibrillar protein:;. The solubility of FF'C was 3% in water and 15% in 5% NaCl. IIowever, the preparation could be solubilized by treatments with alkali (0.2N NaOH) or proteolytic enzymes. Among the enzymes tested for FPC solubilization, pronase was Pound to be the m3st effective both with respect to the extent and the rate of hydrolysis. The FPC displayed good functional properties in terms of emulsifying capacity and wettability. The PER was better thal that obtained for casein when fed to rats at 11% protein level.

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Lipids of the cottonseed. III. Perchloric acid ashing of lipids for the determination of phosphorus

Cited by 3 publications

References 4 publications

Determination of the phosphorus content of lipids

Determination of the phosphorus content of lipids

Effect of Gamma‐irradiation on Lipid Constituents of Autolysing Lamb Liver

Fish Protein Concentrate (FPC) from Bombay Duck Isolated by Radiation‐Heat Combination Procedure: Functional and Nutritional Properties

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