Lipids of Archaebacteria

Langworthy, Thomas A.; Tornabene, T. G.; Holzer, G.

doi:10.1016/s0721-9571(82)80036-7

Cited by 91 publications

(64 citation statements)

References 41 publications

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“…An attempt to measure the incorporation of [methyl-2H] The work reported here, which shows that free acetyl-CoA is not involved in isoprenoid biosynthesis in the eubacterium E. coli, is in sharp contrast with what has been observed in both eukaryotes (Beytia & Porter, 1976) and in archaebacteria (Langworthy et al, 1982;De Rosa et al, 1986;Moldoveanu & Kates, 1988), which use acetyl-CoA in the biosynthesis of isoprenoids. If this pathway for the biosynthesis of isoprenoids is a general characteristic of all eubacteria, then it may represent a clear biochemical marker that separates eubacteria from archaebacteria and eucaryotes.…”

Section: Discussioncontrasting

confidence: 55%

“…The sequence of reactions involved in this important pathway were derived from work with animals and yeasts (Beytia & Porter, 1976) and, more recently, in archaebacteria (Langworthy et al, 1982;De Rosa et al, 1986;Moldoveanu & Kates, 1988). The biosynthesis of isoprenoids has been less extensively studied in the eubacteria; however, current biosynthetic evidence indicates that the steps from IPP to isoprenoids in eubacteria are the same as those in eukaryotes (Fujisaki et al, 1986a,b).…”

Section: Introductionmentioning

confidence: 99%

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Early steps of isoprenoid biosynthesis in Escherichia coli

Zhou

White

1991

Biochemical Journal

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The incorporation of 2H- and 13C-labelled precursors into ubiquinone-8 (Uq-8) by strains of Escherichia coli was measured in order to define the pathway for the early steps in the biosynthesis of isoprenoids in these eubacteria. Cells grown with DL-[methyl-2H6]valine were found to label both the alpha-oxoisovaleric (‘alpha-ketoisovaleric’) acid alpha-oxoisohexanoic (‘alpha-ketoisocaproic’) acid, but not the Uq-8. Since these acids are required for the biosynthesis of isoprenoids by the acetolactate pathway, the operation of this pathway in the biosynthesis of Uq-8 is excluded. Cells grown with [1,2-13C2]acetate and non-labelled glucose readily incorporated 13C2 units into fatty acids, but failed to incorporate any label into the Uq-8. Cells grown with [U-13C6]glucose and non-labelled acetate, however, were found to label both the fatty acids and the Uq-8. Oxidative cleavage with periodate/permanganate of the Uq-8 isolated from cells grown with U-13C6-labelled glucose produced laevulinic acid, which was shown to be derived from two C2 units and one C1 unit of the labelled glucose by mass-spectral analysis of the 4,5-dihydro-6-methyl-2-phenylpyridazin-3(2H)-one derivative. The results of this work indicate that the C-2 and C-3 carbon unit of pyruvate, not acetyl-CoA, is the precursor to isopentenyl pyrophosphate (IPP) in these cells; however, the labelling pattern observed is consistent with the established acetoacetate pathway of isoprenoid biosynthesis. These data, coupled with the observed lack of inhibition of the growth of E. coli by mevinolin, a specific inhibitor of 3-hydroxy-3-methylglutaryl-CoA, can be best rationalized by the biosynthesis of IPP occurring in E. coli through a series of bound intermediates.

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Section: Discussioncontrasting

confidence: 55%

Section: Introductionmentioning

confidence: 99%

Early steps of isoprenoid biosynthesis in Escherichia coli

Zhou

White

1991

Biochemical Journal

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“…Archaea are rich in diether and tetraether lipids (Gliozzi et al, 2002;Kates, 1992;Koga and Morii, 2005;Langworthy et al, 1982;Langworthy and Pond, 1986;Lanzotti et al, 1989;Sprott et al, 1991). The distributions of these ether lipids vary with the archaea classes.…”

Section: Structural Featuresmentioning

confidence: 99%

Archaebacterial bipolar tetraether lipids: Physico-chemical and membrane properties

Chong

2010

Chemistry and Physics of Lipids

124

117

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“…Figure 7 shows the El mass spectrum of diphytanylglycerol diether, a membrane lipid common in archaebacteria [6]. The compound was introduced into the mass spectrometer through the SFC column.…”

Section: Figurementioning

confidence: 99%

Capillary supercritical fluid chromatography with simultaneous flame ionization and mass spectrometric detection

Holzer

DeLuca

Voorhees

1985

J. High Resol. Chromatogr.

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SummaryA simple interface between a capillary supercritical fluid chromatograph and an Extranuclear Simulscan mass spectrometer is described. The SFC column is directly inserted into the ion source through the existing GC-interface. The system is equipped with a splitting device which allows simultaneous El/ MS and flame ionization detection when C 0 2 is used as the supercritical phase. The effect of source temperature and pressure on C02 clustering was studied for optimization of source conditions.The performanceof thesystem wasevaluated witha series of model compounds and standard mixtures.

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Lipids of Archaebacteria

Cited by 91 publications

References 41 publications

Early steps of isoprenoid biosynthesis in Escherichia coli

Early steps of isoprenoid biosynthesis in Escherichia coli

Archaebacterial bipolar tetraether lipids: Physico-chemical and membrane properties

Capillary supercritical fluid chromatography with simultaneous flame ionization and mass spectrometric detection

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