Lipids in the Structure and Function of Yeast Membrane

Prasad, Rajendra

doi:10.1016/b978-0-12-024921-3.50012-5

Cited by 30 publications

(20 citation statements)

References 384 publications

(231 reference statements)

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“…Finally, yeast mitochondria have been found to exert control over the cell-surface complex, influencing agglutination, tolerance to drugs, utilization of sugars and plasma membrane proteins (Prasad, 1985, and references therein). Under 0, limitation, or in the presence of excess reducing equivalents due to oxidative biochemical pro-…”

Section: Cell Wall Structuresmentioning

confidence: 99%

“…(It is possible that during the recovery of these latter cells, much of the outer layer had been washed off.) The synthesis of this outer fibrillar layer has previously been associated with the nutritional status : an intercellular matrix was seen by scanning electron microscopy on the surface of C. albicans when grown as colonies on agar (Joshi e t al., 1975) and growth of C. albicans on various carbohydrates, particularly galactose, in liquid media induced the synthesis of extracellular polymeric material (McCourtie & Douglas, 1981 ;1985).…”

Section: Cell Wall Structuresmentioning

confidence: 99%

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Changes in cell morphology and carnitine acetyltransferase activity in Candida albicans following growth on lipids and serum and after in vivo incubation in mice

Sheridan

Ratledge

1996

Microbiology

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Candida aibicans C316, maintained in the yeast form, showed a proliferation of peroxisomes when grown on triolein or serum as sole carbon source but these structures were absent from glucose-grown cells. Peroxisomes were also apparent in C. aibicans obtained after injection into mice and recovery from intraperitoneal washings and kidneys; they may therefore be useful markers to assess a potential in vivo response in cells that are growing in vitro. Transcell-wall structures also occurred in C. aibicans grown on triolein or serum, and in cells cultured in wiwo, but were not seen in cells grown on glucose. These structures consisted of electron-dense f ibrillar material penetrating through the cell wall from the plasmalemma side and protruded out to the exterior of the cell. Endoplasmic reticulum, located at the periphery of the cell, was found to be in close proximity with these cell wall structures. Carnitine acetyltransferase (CAT; EC 2.3.1.7), the key enzyme for the translocation of acetyl units between intracellular compartments, was present in low activities in glucose-grown cells; its activity was increased some 100-fold in trioleingrown cells but only b o l d in serum-grown cells. It was not possible to assess this activity in the in wivo-cultured cells. Two separate CAT proteins, partially purifed from isolated microchondria and peroxisomes, respectively, were identified, with different specificities and kinetic properties.

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Section: Cell Wall Structuresmentioning

confidence: 99%

Section: Cell Wall Structuresmentioning

confidence: 99%

Changes in cell morphology and carnitine acetyltransferase activity in Candida albicans following growth on lipids and serum and after in vivo incubation in mice

Sheridan

Ratledge

1996

Microbiology

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“…The apparent KT values for all these amino acids were found to range between 100 and 300 flM [96]. The response of various amino acid permeases to altered lipid environment was, however, different ( [71,72,75]; see Chap. 9, this Vol.…”

Section: In Intact Cellsmentioning

confidence: 95%

The Plasma Membrane of Candida albicans: Its Relevance to Transport Phenomenon

Prasad

1991

Candida Albicans

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“…Ergosterol constitutes approximately 5-7 % of total lipid in the plasma membrane of the yeast cells, where it is available for regulation of fluidity and maintenance of the higher-order structure of the membrane, features which are necessary for stable maximal activity of membrane function. In practice, ergosterol was essential for stable construction of the membrane (Prasad, 1985). Needless to say, other lipids such as glycolipids and phospholipids play important roles in membrane function.…”

Section: Discussionmentioning

confidence: 99%

Reduction of ATPase activity accompanied by photodecomposition of ergosterol by near-UV irradiation in plasma membranes prepared from Saccharomyces cerevisiae

1997

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When plasma membranes prepared from the yeast Sacchammyces cerevisiae were exposed to near-UV radiation, photodecomposition of ergosterol and reduction of ATPase activity occurred simultaneously. The V-for ATPase activity decreased markedly with increasing near-UV dosage while the K, value remained constant. When ATPase solubilized from the plasma membrane was exposed to near-UV, the activity remained constant irrespective of dosage, indicating that the ATPase molecule itself was not damaged by near-UV irradiation. The relationship between content of ergosterol and ATPase activity was examined using liposomes constructed with lipids extracted from the membrane. Maximum activity of ATPase was seen at 5% ergosterol in Iiposomes; this activity was 2.5 times greater than that in liposomes without ergosterol. Activity of ATPase bound to liposomes with 5% ergosterol was reduced after near-UV irradiation, while the activity remained unchanged in the case of the liposomes without ergosterol. Fluidity of the liposomes with 5 YO ergosterol also decreased with increasing near-UV dosage. Dosage-response curves for reduction of ATPase activity and for decrease in fluidity were similar to that for photodecomposition of ergosterol. These results suggested that the reduction of ATPase activity in the membrane by near-UV irradiation was not caused by photochemical degradation of the primary structure of the ATPase molecule, but was attributable to conformational change resulting from an alteration in the higher-order structure of the membrane due to photochemical decomposition of ergosterol.

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Lipids in the Structure and Function of Yeast Membrane

Cited by 30 publications

References 384 publications

Changes in cell morphology and carnitine acetyltransferase activity in Candida albicans following growth on lipids and serum and after in vivo incubation in mice

Changes in cell morphology and carnitine acetyltransferase activity in Candida albicans following growth on lipids and serum and after in vivo incubation in mice

The Plasma Membrane of Candida albicans: Its Relevance to Transport Phenomenon

Reduction of ATPase activity accompanied by photodecomposition of ergosterol by near-UV irradiation in plasma membranes prepared from Saccharomyces cerevisiae

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