Lipids as Markers of Induced Resistance in Wheat: A Biochemical and Molecular Approach

Tayeh, Ch.; Randoux, Béatrice; Laruelle, Frédéric; Bourdon, Natacha; Reignault, Ph

doi:10.5772/51279

Cited by 7 publications

(6 citation statements)

References 114 publications

(135 reference statements)

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“…3 d–f, green). At the site of pathogen entry, PAs belong to those defense signaling molecules that rapidly originate from plant membrane lipids [ 44 ].…”

Section: Resultsmentioning

confidence: 99%

Histology-guided high-resolution AP-SMALDI mass spectrometry imaging of wheat-Fusarium graminearum interaction at the root–shoot junction

Bhandari

Wang

et al. 2018

Plant Methods

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BackgroundFungal pathogens like Fusarium graminearum can cause severe yield losses and mycotoxin contamination of food and feed worldwide. We recently showed its ability to systemically colonize wheat via root infection. However, the molecular response of wheat to Fusarium root rot (FRR) infection and systemic spread is still unknown. As a molecular camera, mass spectrometry (MS) imaging combines label-free and multiplex metabolite profiling with histopathology.ResultsAtmospheric-pressure (AP)-SMALDI-MS imaging was combined with optical microscopy to study wheat-F. graminearum interaction at the root–shoot junction, which is a crucial line of defense against a pathogen that can invade all distal plant parts. To scope the functional, temporal and local aspects of FRR disease spread, metabolic changes were simultaneous visualized in diseased and healthy stem bases of the resistant cultivar Florence-Aurore at 10, 14 and 21 days after root inoculation. Histological information was used to identify disease relevant tissues and to assist the interpretation of molecular images. Detected mycotoxin compounds secreted by F. graminearum showed a route of stem infection that was consistent with observations made by microscopy. The outer epidermis and vasculature of leaf sheath were, at different disease stages, identified as prominent sites of pathogen migration and wheat protection. Wheat metabolites mapped to these relatively small tissues indicated cell wall strengthening and antifungal activity as direct defenses as well as conservation in the wheat reactions to F. graminearum diseases that affect different plant organs.ConclusionsAP-SMALDI-MS imaging at high spatial resolution is a versatile technique that can be applied to basic and applied aspects of agricultural research. Combining the technology with optical microscopy was found to be a powerful tool to gain in-depth information on almost unknown crop disease. Moreover, the approach allowed studying metabolism at the host–pathogen interface. The results provide important hints to an understanding of the complex spatio-temporal organization of plant resistance. Defense-on-demand responses to pathogen ingress were found, which provide opportunities for future research towards an improved resistance that does not negatively impact yield development in the field by saving plant resources and, moreover, may control different Fusarium diseases.Electronic supplementary materialThe online version of this article (10.1186/s13007-018-0368-6) contains supplementary material, which is available to authorized users.

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“…3 d–f, green). At the site of pathogen entry, PAs belong to those defense signaling molecules that rapidly originate from plant membrane lipids [ 44 ].…”

Section: Resultsmentioning

confidence: 99%

Histology-guided high-resolution AP-SMALDI mass spectrometry imaging of wheat-Fusarium graminearum interaction at the root–shoot junction

Bhandari

Wang

et al. 2018

Plant Methods

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“…Drought stress reduces the accumulation of fatty acids, such as palmitic acid, hexadecenoic acid, picolinic acid, and stearidonic acid [ 4 , 35 , 36 ], while substantially increasing the levels of most fatty acids, with the exception of pimelic acid, capric acid, (2’E,4’Z,7’Z,8E)-colnelenic acid, alpha-linolenic acid, traumatic acid (TA) and acetyl-CoA carboxylase (ACCase) [ 37 ]. Tayeh et al [ 38 ] showed that phospholipase A1 (PLA1) in plants plays important roles in the hydrolysis of phospholipids during cold stress. Andrade et al [ 39 ] found that lipoxygenase (LOX) is involved in defense against drought stress in rice and sugarcane transgenic plants.…”

Section: Discussionmentioning

confidence: 99%

Integrated omics data of two annual ryegrass (Lolium multiflorum L.) genotypes reveals core metabolic processes under drought stress

et al. 2018

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Background: Annual ryegrass (Lolium multiflorum L.) is a commercially important, widely distributed forage crop that is used in the production of hay and silage worldwide. Drought has been a severe environmental constraint in its production. Nevertheless, only a handful of studies have examined the impact of short-term drought stress on annual ryegrass. The aim of this study was to explore how stress-induced core metabolic processes enhance drought tolerance, or adaptation to drought, in annual ryegrass.

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“…61 Changes in these phospholipids occur at the site of fungal infection, since they are rapidly formed in response to pathogen attacks, which makes them useful markers for induced resistance. 62 Therefore, spatial distributions in diseased tissues could facilitate the identification of resistance-related lipids.…”

Section: Wheat Stem Basementioning

confidence: 99%

High resolution mass spectrometry imaging of plant tissues: towards a plant metabolite atlas

Bhandari

Wang

Friedt

et al. 2015

Analyst

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Mass spectrometry (MS) imaging provides spatial and molecular information for a wide range of compounds. This tool can be used to investigate metabolic changes in plant physiology and environmental interactions. A major challenge in our study was to prepare tissue sections that were compatible with high spatial resolution analysis and therefore dedicated sample preparation protocols were established and optimized for the physicochemical properties of all major plant organs. We combined high spatial resolution (5 μm), in order to detect cellular features, and high mass accuracy (<2 ppm root mean square error), for molecular specificity. Mass spectrometry imaging experiments were performed in positive and negative ion mode. Changes in metabolite patterns during plant development were investigated for germination of oilseed rape. The detailed localization of more than 90 compounds allowed assignment to metabolic processes and indicated possible functions in plant tissues. The 'untargeted' nature of MS imaging allows the detection of marker compounds for the physiological status, as demonstrated for plant-pathogen interactions. Our images show excellent correlation with optical/histological examination. In contrast to previous MS imaging studies of plants, we present a complete workflow that covers multiple species, such as oilseed rape, wheat seed and rice. In addition, different major plant organs and a wide variety of compound classes were analyzed. Thus, our method could be used to develop a plant metabolite atlas as a reference to investigate systemic and local effects of pathogen infection or environmental stress.

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Lipids as Markers of Induced Resistance in Wheat: A Biochemical and Molecular Approach

Cited by 7 publications

References 114 publications

Histology-guided high-resolution AP-SMALDI mass spectrometry imaging of wheat-Fusarium graminearum interaction at the root–shoot junction

Histology-guided high-resolution AP-SMALDI mass spectrometry imaging of wheat-Fusarium graminearum interaction at the root–shoot junction

Integrated omics data of two annual ryegrass (Lolium multiflorum L.) genotypes reveals core metabolic processes under drought stress

High resolution mass spectrometry imaging of plant tissues: towards a plant metabolite atlas

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