Lipids and fatty acids of three species of northeast pacific finfish harvested in summer

Wander, Rosemary C.; Patton, Beverly D.

doi:10.1016/0889-1575(91)90006-r

Cited by 7 publications

(5 citation statements)

References 17 publications

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“…The increased catalytic activity of bbox1 in RFP indicates fast lipid oxidization and decreased fat accumulation in RFP and thus may correlate with the flat phenotype, as observed in other teleosts 77 , 78 . This hypothesis was further supported by the lower whole body and muscular fat content in RFP compared with other nonflatfish teleosts, as observed in our analysis and in multiple other studies 79 – 81 , since a significantly lower fat content ( P < 0.001) was observed in RFP than in nonflatfish outgroups in both whole body (6.22-fold low) and muscular (5.76-fold low) tissues (Fig. 3e ; see Supplementary Note 24 ).…”

Section: Resultssupporting

confidence: 91%

Large-scale sequencing of flatfish genomes provides insights into the polyphyletic origin of their specialized body plan

et al. 2021

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The evolutionary and genetic origins of the specialized body plan of flatfish are largely unclear. We analyzed the genomes of 11 flatfish species representing 9 of the 14 Pleuronectiforme families and conclude that Pleuronectoidei and Psettodoidei do not form a monophyletic group, suggesting independent origins from different percoid ancestors. Genomic and transcriptomic data indicate that genes related to WNT and retinoic acid pathways, hampered musculature and reduced lipids might have functioned in the evolution of the specialized body plan of Pleuronectoidei. Evolution of Psettodoidei involved similar but not identical genes. Our work provides valuable resources and insights for understanding the genetic origins of the unusual body plan of flatfishes.

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Section: Resultssupporting

confidence: 91%

Large-scale sequencing of flatfish genomes provides insights into the polyphyletic origin of their specialized body plan

et al. 2021

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“…The supplement (15 g) provided 1.26 g EPA and 1.08 g DHA. This amount (2.34 g) of n-3 FA is similar to that found in two servings (200 g) of Chinook salmon (27). FO supplements were kindly provided by OmegaPure TM (Houston, TX) in bulk and were then encapsulated by Banner Pharmacaps, Inc. (High Point, NC).…”

Section: Methodsmentioning

confidence: 99%

Enrichment of LDL with EPA and DHA decreased oxidized LDL‐induced apoptosis in U937 cells

Geigerman

Lee

et al. 2002

Lipids

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Oxidized LDL (oxLDL) may contribute to the accumulation of apoptotic cells in atherosclerotic plaques. Although it is well established in monophasic chemical systems that the highly unsaturated EPA and DHA will oxidize more readily than FA that contain fewer double bonds, our previous studies showed that enrichment of LDL, which has discrete polar and nonpolar phases, with these FA did not increase oxidation. The objective of this study was to compare the extent of apoptosis induced by EPA/DHA-rich oxLDL to that induced by EPA/DHA-non-rich oxLDL in U937 cells. LDL was obtained from one healthy subject three times before and after supplementation for 5 wk with 15 g/d of fish oil (FO), an amount easily obtainable from a diet that contains fatty fish. After supplementation, an EPA/DHA-rich LDL was obtained. Oxidative susceptibility of LDL, as determined by measuring the formation of conjugated dienes and the accumulation of cholesteryl ester hydroperoxides, was not higher in EPA/DHA-rich LDL. The oxLDL-induced cell apoptosis was detected by the activation of caspase-3, the translocation of PS to the outer surface of the plasma membrane using the Annexin V-fluorescein isothiocyanate binding assay, and the presence of chromatin condensation and nuclear fragmentation using the 4,6-diamidino-2-phenylindole staining assay. All three measures showed that after FO supplementation, EPA/DHA-rich oxLDL-induced cell apoptosis decreased. The decrease was not related to the concentration of lipid hydroperoxides. This study suggests that a possible protective effect of EPA/DHA-rich diets on atherosclerosis may be through lessening cell apoptosis in the arterial wall.

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“…The fatty acid profile of the oils was measured by gas chromatography using heptadecanoic acid (NuChek Prep, Elysian, MN) as an internal standard as described previously [30]. The intake of monounsaturated fatty acids (MUFA) provided by the supplements was similar in all three groups.…”

Section: Supplementationmentioning

confidence: 99%

“…The plasma fatty acid profile was measured by gas chromatography as previously described by Song and Wander [30]. Heptadecanoic acid (NuChek Prep) was added as an internal standard.…”

Section: Analysis Of Plasmamentioning

confidence: 99%

Reduced effect on apoptosis of 4-hydroxyhexenal and oxidized LDL enriched with n-3 fatty acids from postmenopausal women

Lee

Wander

2005

The Journal of Nutritional Biochemistry

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Background: Oxidized low-density lipoprotein (oxLDL) promotes apoptosis in atherosclerotic plaques in the vascular wall, a process mediated through its oxidized lipids. 4-Hydroxynonenal (HNE) and 4-hydroxyhexenal (HHE), derived from oxidation of n-6 and n-3 fatty acids, respectively, are among the major oxidized products in oxLDL.Hypothesis: This study hypothesized that eicosapentaenoic acid/docosahexaenoic acid (EPA/DHA)-rich versus linoleic acid-rich oxLDL obtained from postmenopausal women and HNE versus HHE differentially influence apoptosis in U937 cells.Experimental Design: Thirty healthy postmenopausal women were supplemented with 14 g/day safflower oil (SO), 7 g/day of both fish oil and SO (low dose LFO) or 14 g/day fish oil (high dose HFO) for 5 weeks. Low-density lipoprotein, obtained after supplementation, was oxidized with 5 µM CuSO4 at 37°C for 6 h. The concentration of cholesteryl ester hydroperoxides (CEOOH) and conjugated dienes was measured in the oxidized LDL (oxLDL). U937 cells were incubated with the oxLDL, 10 µM of HHE, 7 µM of HHE plus 3 µM of HNE, 5 µM of both HHE and HNE or 10 µM of HNE and the extent of apoptosis measured three ways. Results:The concentration of CEOOH and conjugated dienes in oxLDL did not differ among the three treatment groups. The percent of apoptotic cells was approximately 40% lower when incubated with oxLDL obtained from the HFO-supplemented group than the SO-supplemented group measured by both the Annexin V and the DNA fragmentation assays (P=.04 and .004, respectively). Apoptosis of U937 cells was significantly lower in cells incubated with 10 µM of HHE, and mixtures of HHE and HNE than the 10 µM HNE when measured by the Annexin V, DNA fragmentation and 4,6-diamidino-2-phenylindole (DAPI) staining. Conclusions:These data suggest that the cardioprotective properties of n-3 fatty acids may derive in part from their less reactive oxidized lipid metabolites.

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Lipids and fatty acids of three species of northeast pacific finfish harvested in summer

Cited by 7 publications

References 17 publications

Large-scale sequencing of flatfish genomes provides insights into the polyphyletic origin of their specialized body plan

Large-scale sequencing of flatfish genomes provides insights into the polyphyletic origin of their specialized body plan

Enrichment of LDL with EPA and DHA decreased oxidized LDL‐induced apoptosis in U937 cells

Reduced effect on apoptosis of 4-hydroxyhexenal and oxidized LDL enriched with n-3 fatty acids from postmenopausal women

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