Lipidomics perturbations in the brain of adult zebrafish (Danio rerio) after exposure to chiral ibuprofen

Zhang, Wei; Song, Yue; Chai, Tuanyao; Liao, Guangqin; Zhang, Lin; Jia, Qi; Qian, Yongzhong; Qiu, Jing

doi:10.1016/j.scitotenv.2020.136565

Cited by 30 publications

(17 citation statements)

References 72 publications

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“…This analysis is important to undertake throughout the exposure period. Mennillo et al (2018) reported no change to the concentration of S(+)-ketoprofen in P. subcapitata test media after 72 h. Zhang et al (2020) also reported drug stability during exposure of D. rerio with ibuprofen enantiomers. Although they confirmed the 'total' ibuprofen concentration did not vary substantially, enantioselective analysis was not used to confirm the enantiomeric composition and confirm inversion did not take place.…”

Section: Ecotoxicity Studiesmentioning

confidence: 96%

“…However, toxicity was not enantiospecific with EC 50 values (% inhibition, 30 min) of 3.1 and 2.8 mg L −1 for S(−)-ibuprofen and R(+)-ibuprofen, respectively (Neale et al 2019). However, studies on zebra fish (Danio rerio) have reported significant differences on lipid metabolites in brain tissue induced by S(−)-ibuprofen and R(+)-ibuprofen (Zhang et al 2020). In this study, the test fish were exposed to environmentally relevant enantiomer concentrations (5 µg L −1 ) for 28 days.…”

Section: Enantiospecific Toxicitymentioning

confidence: 99%

“…In this study, the test fish were exposed to environmentally relevant enantiomer concentrations (5 µg L −1 ) for 28 days. The authors concluded that ibuprofen can induce enantiospecific toxicity to aquatic organisms (Zhang et al 2020).…”

Section: Enantiospecific Toxicitymentioning

confidence: 99%

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Analysis, fate and toxicity of chiral non-steroidal anti-inflammatory drugs in wastewaters and the environment: a review

Petrie

Camacho‐Muñoz

2020

Environ Chem Lett

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Non-steroidal anti-inflammatory drugs (NSAIDs) are found in the aquatic environment globally. Such drugs including naproxen, ibuprofen and ketoprofen are chiral molecules. Enantiomers of those drugs have identical physicochemical properties but can behave and interact differently in chiral environments due to differences in their three-dimensional shape. This results in enantiospecific differences in environmental fate and toxicity, which is often overlooked. Therefore, we review the analytical methods, occurrence and fate, and toxicity of chiral non-steroidal anti-inflammatory drugs at the enantiomeric level. The advancement of enantioselective chromatography methods, particularly the use of polysaccharide-based stationary phases, has enabled trace determination of enantiomers in complex environmental matrices. Macrocosm and microcosm studies of engineered and natural environments revealed that such drugs can undergo both enantioselective degradation and chiral inversion. Enantioselectivity has been reported during wastewater treatment, in surface waters and in agricultural soils. The use of microcosms spiked with individual enantiomers over racemates is essential to evaluate these degradation and inversion fate processes. The chiral inversion process whereby one enantiomer converts into its antipode can be significant if the more toxic enantiomers are formed. Existing enantiospecific effect studies report less than an order of magnitude difference in enantiomer toxicity. However, toxicity data for enantiomers are limited and further research is needed to better appreciate the environmental risk at the enantiomeric level.

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Section: Ecotoxicity Studiesmentioning

confidence: 96%

Section: Enantiospecific Toxicitymentioning

confidence: 99%

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Analysis, fate and toxicity of chiral non-steroidal anti-inflammatory drugs in wastewaters and the environment: a review

Petrie

Camacho‐Muñoz

2020

Environ Chem Lett

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“…The authors used a combination of 1 H-NMR for the analysis of monosaccharides, amino acids, and organic acids, GC-MS for cholesterol and fatty acids, and LC-MS for the analysis of lipids. However, more recent zebrafish toxicometabolomics studies (≥2017) reported using LC-HRMS (Orbitrap or QTOF) [ 15 , 28 , 29 , 31 , 33 , 36 , 48 , 51 , 85 , 86 , 87 , 88 ], followed by 1 H-NMR [ 24 , 30 , 89 , 90 , 91 , 92 , 93 ] and GC-HRMS [ 21 , 94 ], while multi-analytical platform studies remain scarce.…”

Section: Experimental Designmentioning

confidence: 99%

Mass Spectrometry-Based Zebrafish Toxicometabolomics: A Review of Analytical and Data Quality Challenges

et al. 2021

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Metabolomics has achieved great progress over the last 20 years, and it is currently considered a mature research field. As a result, the number of applications in toxicology, biomarker, and drug discovery has also increased. Toxicometabolomics has emerged as a powerful strategy to provide complementary information to study molecular-level toxic effects, which can be combined with a wide range of toxicological assessments and models. The zebrafish model has gained importance in recent decades as a bridging tool between in vitro assays and mammalian in vivo studies in the field of toxicology. Furthermore, as this vertebrate model is a low-cost system and features highly conserved metabolic pathways found in humans and mammalian models, it is a promising tool for toxicometabolomics. This short review aims to introduce zebrafish researchers interested in understanding the effects of chemical exposure using metabolomics to the challenges and possibilities of the field, with a special focus on toxicometabolomics-based mass spectrometry. The overall goal is to provide insights into analytical strategies to generate and identify high-quality metabolomic experiments focusing on quality management systems (QMS) and the importance of data reporting and sharing.

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“…Research in lipidomics (including biomarkers and therapeutics) has been hampered by difficulties in extracting, identifying and quantifying lipids. Previous studies have focused on the use of mass spectrometry techniques and while 1 H-NMR has been conducted on zebrafish embryos this has mainly been to study polar/hydrophilic metabolites [15][16][17][18][19]. Robust methods for extraction and collection of 1 H-NMR spectra are essential.…”

Section: Introductionmentioning

confidence: 99%

A robust method for performing 1H-NMR lipidomics in embryonic zebrafish

et al. 2021

Preprint

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The embryonic zebrafish is an ideal system for lipid analyses with relevance to many areas of bioscience research, including biomarkers and therapeutics. Research in this area has been hampered by difficulties in extracting, identifying and quantifying lipids. We employed 1H-NMR at 700MHz to profile lipids in developing zebrafish embryos. The optimal method for lipidomics in embryonic zebrafish incorporated rapid lipid extraction using chloroform and an environment without oxygen depletion. Pools of 10 embryos gave the most acceptable signal-to-noise ratio, and the inclusion of chorions in the sample had no significant effect on lipid abundances. Embryos, bisected into cranial (head and yolk sac) and caudal (tail) regions, were compared by principal component analysis and analysis of variance. The lipid spectra (including lipid annotation) are available in the public repository MetaboLights (MTBLS2396)

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Lipidomics perturbations in the brain of adult zebrafish (Danio rerio) after exposure to chiral ibuprofen

Cited by 30 publications

References 72 publications

Analysis, fate and toxicity of chiral non-steroidal anti-inflammatory drugs in wastewaters and the environment: a review

Analysis, fate and toxicity of chiral non-steroidal anti-inflammatory drugs in wastewaters and the environment: a review

Mass Spectrometry-Based Zebrafish Toxicometabolomics: A Review of Analytical and Data Quality Challenges

A robust method for performing 1H-NMR lipidomics in embryonic zebrafish

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