Lipidomics Identified Lyso-Phosphatidylcholine and Phosphatidylethanolamine as Potential Biomarkers for Diagnosis of Laryngeal Cancer

Abstract

BackgroundLaryngeal cancer (LaC) remains one of the most common tumors of the respiratory tract with higher incidence in men than in women. The larynx is a small but vital organ on the neck. The dysfunction of the larynx can cause serious health problems such as hoarseness, respiratory distress, and dysphonia. Many lipids (e.g. phospholipid, cholesterol, fatty acid) have been recognized as a crucial role in tumorigenesis. However, the lipid biomarkers are lacking and the lipid molecular pathogenesis of LaC is still unclear.MethodsThis study aims to identify new LaC-related lipid biomarkers used for the diagnosis or early diagnosis of LaC and to uncover their molecular characteristics. Thus, we conducted serum and tissue nontargeted lipidomics study from LaC patients (n = 29) and normal controls (NC) (n = 36) via ultra-high performance liquid chromatography (UHPLC) coupled with high resolution mass spectrometry (HRMS). Multivariate and univariate statistics analyses were used to discriminate LaC patients from NC.ResultsAs expected, a lipid panel including LPC (16:0) and PE (18:0p_20:4) was defined to distinguish the LaC patients from healthy individuals with very high diagnosis performance (area under the curve (AUC) value = 1.000, sensitivity value = 1.000, and specificity value = 1.000). In addition, the levels of Cer, CerG1, SM, PC, PC-O, PE, PI, PS, and ChE in the LaC group significantly increased as compared with the NC group. However, the levels of LPC, LPC-O, LPE, LPE-p, and DG in the LaC group significantly deceased when the one was compared with the NC group. Among significantly changed lipid species, lysophospholipids containing a palmitoyl chain or an arachidonic acid acyl chain remarkably decreased and phospholipids including a palmitoyl chain or an arachidonic acid acyl chain increased in the LaC patients.ConclusionOur results not only indicate that lipidomics is powerful tool to explore abnormal lipid metabolism for the laC, but suggest that lysophospholipids and phospholipids may serve as potential biomarkers for diagnosis of LaC.

MALDI Mass Spectrometry Imaging Highlights Specific Metabolome and Lipidome Profiles in Salivary Gland Tumor Tissues

“…Moreover, the levels of Lysophosphatidylcholines (LPC) 16:0 decreased in the tumor in comparison to the adjacent healthy tissue. This result is consistent with previous relevant findings in which LPC 16:0 significantly decreased in laryngeal cancer serum samples and prostate cancer tissues, highlighting the early diagnostic potential of this lipid biomarker [33,35]. This point can be supported by the mutual conversion of PCs and LPCs.…”

“…These findings are in good agreement with previous studies that have proposed the spatial confinement of PCs to specific cancer models [29][30][31]. It is well known that PCs are the most predominant components of phospholipids for biological membranes; PC metabolism is altered in the onset and development of many tumors [32,33]. In particular, the elevated levels of PC 32:1 and PC 34:1 have been positively correlated to the cancerous areas of colorectal, prostate, and breast cancer tissues [29][30][31].…”

“…This point can be supported by the mutual conversion of PCs and LPCs. In fact, upregulating the PC level, which may come from LPC conversion, the LPC level for the tumor decreases [33].…”

Identification and characterization of lysophosphatidylcholine 14:0 as a biomarker for drug-induced lung disease

“…Our results further strengthen the association between blood LPC levels and acute inflammation. Moreover, LPCs have been reported as candidate biomarkers for cancer and diabetes, with which chronic inflammation is associated 25,31,32 . However, we found that plasma LPC(14:0) concentrations were higher in patients with the chronic inflammatory lung diseases IIP, CTD, COPD, and BrAs, than that in patients with DILD.…”

Integrated Transcriptomics and Metabolomics Analyses of Stress-Induced Murine Hair Follicle Growth Inhibition

“…The upregulated genes included Gpx7, Gpx3, Cyp2e1, Ptges, Ptgis while Ptgds, Plb1, Cyp2b19, Ggt1, Pla2g2e, Pla2g2d, Alox12 were dramatically downregulated (Figures 6A, 7A, 8A). Arachidonic acid is one of the major polyunsaturated fatty acids in mammals (Yu and Wang, 2021). In consideration of the significant decrease of AA-residue-enriched LPCs (e.g., LysoPC…”