Lipidomics for wildlife disease etiology and biomarker discovery: a case study of pansteatitis outbreak in South Africa

Koelmel, Jeremy P.; Ulmer, Candice Z.; Fogelson, Susan B.; Jones, Christina M.; Botha, Hannes; Bangma, Jacqueline T.; Guillette, Theresa C.; Luus‐Powell, Wilmien J.; Sara, Joseph R.; Smit, Willem J.; Albert, Korin; Miller, Harmony A.; Guillette, Matthew P.; Olsen, Berkley C.; Cochran, Jason A.; Garrett, Timothy J.; Yost, Richard A.; Bowden, John A.

doi:10.1007/s11306-019-1490-9

Cited by 12 publications

(8 citation statements)

References 40 publications

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“…Adipocyte degeneration characterized by macrophage infiltration and oxidative damage observed in Mozambique tilapia in this study is further evidenced by lipidomics work conducted on the same species within the same impoundment by Koelmel et al. (2019). Koelmel et al.…”

Section: Discussionsupporting

confidence: 77%

“…As with C. gariepinus (Huchzermeyer, 2012b; Huchzermeyer et al., 2013), the progression of pansteatitis in Mozambique tilapia begins with the inflammation of individual adipocytes and the disintegration of the clear intracytoplasmic lipid vacuole into numerous smaller foamy vacuoles (Koelmel et al., 2019). Intracellular contents are further characterized as ceroid accumulation within granulomata and the cytoplasm of infiltrating or aggregated macrophages (Huchzermeyer et al., 2017).…”

Section: Discussionmentioning

confidence: 99%

“…Koelmel et al. (2019) revealed that glycerophospholipids contained in macrophage cell membranes increased, with increased disease severity, as well as short chained oxidized triacylglycerol species. Cell inflammation and necrosis were indicated by an exponential increase in sphingolipids as a factor of the allotted pansteatitis veterinary score (Koelmel et al., 2019).…”

Section: Discussionmentioning

confidence: 99%

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A histological evaluation of pansteatitis‐affected Mozambique tilapia, Oreochromis mossambicus (Peters 1852), from different geographical locations in South Africa

Sara

Luus‐Powell

Fogelson³

et al. 2020

Journal of Fish Diseases

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Pansteatitis is the leading cause for the decline in Nile crocodile populations and the sporadic mortality of fish in the Olifants River System, South Africa. To determine the prevalence of this disease in lentic systems, Mozambique tilapia, Oreochromis mossambicus, were collected from Lake Loskop, Lake Flag Boshielo, Phalaborwa Barrage and Lake Luphephe-Nwanedi. The former three impoundments are located within the main stem of the Olifants River, while the latter, which is geographically isolated and situated in the Limpopo River System, served as a reference site. Mesenteric adipose, liver, serosa of the swim bladder, gill and the skeletal muscle of fish sampled were examined for gross and microscopic evidence of pansteatitis. Microscopically observed changes were used to statistically compare pansteatitis prevalence between samples and sites. Based on histopathological evaluation, the adipose tissue in the liver, swim bladder serosa and coelom from severely debilitated individuals showed the most significant pathological changes. Lesions indicative of steatitis were observed in fish collected from Lake Loskop (75%), Lake Flag Boshielo (22%) and Lake Luphephe-Nwanedi (15%). Further investigation is warranted to understand the pervasiveness and mechanisms driving pathological changes of pansteatitis at Lake Flag Boshielo, Phalaborwa Barrage and Lake Luphephe-Nwanedi.

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Section: Discussionsupporting

confidence: 77%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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A histological evaluation of pansteatitis‐affected Mozambique tilapia, Oreochromis mossambicus (Peters 1852), from different geographical locations in South Africa

Sara

Luus‐Powell

Fogelson³

et al. 2020

Journal of Fish Diseases

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“…In recent years, the emergence and adoption of the "omics" fields, such as lipidomics, has dramatically increased our understanding of the relationship between chemical and environmental exposure of animal diseases such as pansteatitis (Koelmel et al 2019), oxidative stress (Marqueño et al 2019), and inflammation (Pasamontes et al 2017). Lipidomics, as an omics discipline, aims to measure all the molecules that can be classified as lipids within a biological sample (Han and Gross 2003).…”

Section: Introductionmentioning

confidence: 99%

“…Lipidomics can be used to examine changes in lipid metabolites that may be indicative of altered physiological states such as inflammation or disease (Koelmel et al 2019).…”

Section: Introductionmentioning

confidence: 99%

Lipidomics reveals specific lipid molecules associated with cold stress syndrome in the Florida manatee (Trichechus manatus latirostris)

et al. 2021

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Cold stress syndrome (CSS) in the Florida manatee (Trichechus manatus latirostris) results in perturbations to many physiological pathways, often leading to further illness or death. In this study, we applied a non-targeted lipidomics approach with ultra-high performance liquid chromatography and high-resolution tandem mass spectrometry to characterize changes related to CSS in the lipidomic profiles of manatee plasma. Lipidomic analyses were conducted on healthy manatee (control) and cold-exposed manatee plasma samples with varying concentrations of Serum Amyloid A (SAA), an acute-phase protein that is associated with inflammatory disease. Control manatees (n = 10) were compared to all manatees exposed to cold temperatures (n = 17), and a subset of those manatees with SAA values > 120 μg/mL (n = 9). Increased SAA values were associated with higher levels of various acylcarnitine lipids, while several triacylglycerols and oxidized triacylglycerols were significantly lower in manatees with cold exposure. These identified lipids are critical molecules involved in the maintenance of energy homeostasis and could potentially be examined in conjunction with current physical parameters to characterize cold stress. The ability to detect such differences highlights the addition of lipidomics as a valuable tool in understanding cold stress and potentially other illnesses in manatees. Further investigation into the function of the altered lipids could greatly increase our understanding of lipid metabolism in physiologically stressed manatees as well as other marine mammals and inform future management recovery strategies.

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Mass Spectrometry‐Driven Lipidomics for Biomarker, Molecular Mechanism, and Therapy

Yang

Qiu

Zhang

et al. 2021

Mass Spectrometry‐Based Metabolomics in Clinical and Herbal Medicines

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Lipidomics for wildlife disease etiology and biomarker discovery: a case study of pansteatitis outbreak in South Africa

Cited by 12 publications

References 40 publications

A histological evaluation of pansteatitis‐affected Mozambique tilapia, Oreochromis mossambicus (Peters 1852), from different geographical locations in South Africa

A histological evaluation of pansteatitis‐affected Mozambique tilapia, Oreochromis mossambicus (Peters 1852), from different geographical locations in South Africa

Lipidomics reveals specific lipid molecules associated with cold stress syndrome in the Florida manatee (Trichechus manatus latirostris)

Mass Spectrometry‐Driven Lipidomics for Biomarker, Molecular Mechanism, and Therapy

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