Lipidomics

Benedusi, Valeria

doi:10.13070/mm.en.8.2665

Cited by 3 publications

(6 citation statements)

References 327 publications

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“…Since most of the extraction protocols described below are based on the SPE and LLE methods, the question arises—which of these protocols is more suitable for lipidomics studies. The advantages and disadvantages of PPT, LLE and SPE have been reviewed comprehensively elsewhere [ 91 , 92 , 93 , 94 , 95 , 96 ] and discussions on which of the methods is better are still ongoing. Among the main advantages of the LLE, one can note its low costs and the presence of a large number of well-established protocols.…”

Section: Methods Of Sample Preparation For Lipidomic Studiesmentioning

confidence: 99%

Sample Preparation Methods for Lipidomics Approaches Used in Studies of Obesity

et al. 2020

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Obesity is associated with alterations in the composition and amounts of lipids. Lipids have over 1.7 million representatives. Most lipid groups differ in composition, properties and chemical structure. These small molecules control various metabolic pathways, determine the metabolism of other compounds and are substrates for the syntheses of different derivatives. Recently, lipidomics has become an important branch of medical/clinical sciences similar to proteomics and genomics. Due to the much higher lipid accumulation in obese patients and many alterations in the compositions of various groups of lipids, the methods used for sample preparations for lipidomic studies of samples from obese subjects sometimes have to be modified. Appropriate sample preparation methods allow for the identification of a wide range of analytes by advanced analytical methods, including mass spectrometry. This is especially the case in studies with obese subjects, as the amounts of some lipids are much higher, others are present in trace amounts, and obese subjects have some specific alterations of the lipid profile. As a result, it is best to use a method previously tested on samples from obese subjects. However, most of these methods can be also used in healthy, nonobese subjects or patients with other dyslipidemias. This review is an overview of sample preparation methods for analysis as one of the major critical steps in the overall analytical procedure.

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Section: Methods Of Sample Preparation For Lipidomic Studiesmentioning

confidence: 99%

Sample Preparation Methods for Lipidomics Approaches Used in Studies of Obesity

et al. 2020

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“…12 Researchers refer to the entire collection of lipid species in a cell, organ, or biological system as a "lipidome" and the large-scale analytical study of this lipidome as well as the lipids' interactions with other lipids, proteins, and metabolites as "lipidomics". 2,3 It was only approximately 40 years ago that lipidomics experiments were made possible due to technological advancements in mass spectrometry (MS). Before this, traditional lipid analysis was performed using techniques such as gas chromatography (GC), high-performance liquid chromatography, thin-layer chromatography, nuclear magnetic resonance and other spectroscopic methods, or MS using electron ionisation, chemical ionisation, and field desorption.…”

Section: Introductionmentioning

confidence: 99%

“…Lipids are dynamic, ubiquitous molecules that play vital roles across all biological systems. They perform the following essential functions within cells: energy storage and metabolism, building blocks of cellular and subcellular membranes and intra‐ and inter‐cellular signalling 1–4 . Mammalian cells express tens to hundreds of thousands of different lipid species 5,6 .…”

Section: Introductionmentioning

confidence: 99%

“…As of 13 th January 2023, the LIPID MAPS Structure Database (LMSD), which is the largest online lipid database, contains 47,800 unique lipid structures 12 . Researchers refer to the entire collection of lipid species in a cell, organ, or biological system as a “lipidome” and the large‐scale analytical study of this lipidome as well as the lipids’ interactions with other lipids, proteins, and metabolites as “lipidomics” 2,3 …”

Section: Introductionmentioning

confidence: 99%

“…They perform the following essential functions within cells: energy storage and metabolism, building blocks of cellular and subcellular membranes and intra‐ and inter‐cellular signalling. 1 , 2 , 3 , 4 Mammalian cells express tens to hundreds of thousands of different lipid species. 5 , 6 Endogenous and exogenous factors, such as age, diet, disease, genetics, and environment can influence lipid composition or expression, which means as molecules, they present unique signatures of invaluable information regarding cellular status that is of high interest for the wider scientific community.…”

Section: Introductionmentioning

confidence: 99%

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Chewing the fat: How lipidomics is changing our understanding of human health and disease in 2022

Géhin

Fowler

Trivedi

2023

Analytical Science Advances

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Lipids are biological molecules that play vital roles in all living organisms. They perform many cellular functions, such as 1) forming cellular and subcellular membranes, 2) storing and using energy, and 3) serving as chemical messengers during intra‐ and inter‐cellular signal transduction. The large‐scale study of the pathways and networks of cellular lipids in biological systems is called “lipidomics” and is one of the fastest‐growing omics technologies of the last two decades. With state‐of‐the‐art mass spectrometry instrumentation and sophisticated data handling, clinical studies show how human lipid composition changes in health and disease, thereby making it a valuable medium to collect for clinical applications, such as disease diagnostics, therapeutic decision‐making, and drug development. This review gives a comprehensive overview of current workflows used in clinical research, from sample collection and preparation to data and clinical interpretations. This is followed by an appraisal of applications in 2022 and a perspective on the exciting future of clinical lipidomics.

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Organic biogeochemistry in the oxygen-deficient ocean: A review

Wakeham

2020

Organic Geochemistry

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Lipidomics

Cited by 3 publications

References 327 publications

Sample Preparation Methods for Lipidomics Approaches Used in Studies of Obesity

Sample Preparation Methods for Lipidomics Approaches Used in Studies of Obesity

Chewing the fat: How lipidomics is changing our understanding of human health and disease in 2022

Organic biogeochemistry in the oxygen-deficient ocean: A review

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