Lipidomics as a new approach for the bioprospecting of marine macroalgae — Unraveling the polar lipid and fatty acid composition of Chondrus crispus

Melo, Tânia; Alves, Eliana; Azevedo, Vítor; Martins, Ana Sofia; Neves, Bruno Miguel; Domingues, Pedro; Calado, Ricardo; Abreu, Helena; Domingues, M. Rosário M.

doi:10.1016/j.algal.2015.02.016

Cited by 88 publications

(97 citation statements)

References 71 publications

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“…However, the majority of published works only identified a few species of glycolipids, either by using offline TLC–MS [30,40,42,43] or selected solvent extraction and MS analysis [15,20,29]. More recently, a detailed profile of Chondrus crispus was reported using LC–MS and MS/MS [26]. …”

Section: Resultsmentioning

confidence: 99%

“…Nowadays, the detailed structural characterization of lipids can be accomplished by using mass spectrometry (MS) coupled with liquid chromatography (LC). This lipidomic approach has the advantage of providing a detailed analysis of the lipid profile and affording the identification and quantification of more than 200 lipid molecular species in one single LC–MS run [25,26]. This detailed information on the specificity of molecular species and corresponding classes of polar lipids cannot be achieved using traditional approaches, typically based on the previous separation of polar lipid classes by thin-layer chromatography (TLC) and silica gel on column chromatography, followed by off-line gas chromatography-mass spectrometry (GC–MS) analysis of FAs [27,28,29,30,31,32,33].…”

Section: Introductionmentioning

confidence: 99%

“…MS-based technologies allowed researchers to explore the full lipidomic signature of distinct matrices [34,35,36]. To date, they allowed for the identification of the full lipidome signature of cultivated seaweeds Ulva lactuca Linnaeus, 1753 [37], Chondrus crispus Stackhouse, 1797 [26], and Codium tomentosum Stackhouse, 1797 [25]. These novel approaches based on specific identification and quantification at the molecular level using high-throughput analysis are promising tools for bioprospection [3,38,39].…”

Section: Introductionmentioning

confidence: 99%

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Valorization of Lipids from Gracilaria sp. through Lipidomics and Decoding of Antiproliferative and Anti-Inflammatory Activity

et al. 2017

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The lipidome of the red seaweed Gracilaria sp., cultivated on land-based integrated multitrophic aquaculture (IMTA) system, was assessed for the first time using hydrophilic interaction liquid chromatography-mass spectrometry and tandem mass spectrometry (HILIC–MS and MS/MS). One hundred and forty-seven molecular species were identified in the lipidome of the Gracilaria genus and distributed between the glycolipids classes monogalactosyl diacylglyceride (MGDG), digalactosyl diacylglyceride (DGDG), sulfoquinovosyl monoacylglyceride (SQMG), sulfoquinovosyl diacylglyceride (SQDG), the phospholipids phosphatidylcholine (PC), lyso-PC, phosphatidylglycerol (PG), lyso-PG, phosphatidylinositol (PI), phosphatidylethanolamine (PE), phosphatic acid (PA), inositolphosphoceramide (IPC), and betaine lipids monoacylglyceryl- and diacylglyceryl-N,N,N-trimethyl homoserine (MGTS and DGTS). Antiproliferative and anti-inflammatory effects promoted by lipid extract of Gracilaria sp. were evaluated by monitoring cell viability in human cancer lines and by using murine macrophages, respectively. The lipid extract decreased cell viability of human T-47D breast cancer cells and of 5637 human bladder cancer cells (estimated half-maximal inhibitory concentration (IC50) of 12.2 μg/mL and 12.9 μg/mL, respectively) and inhibited the production of nitric oxide (NO) evoked by the Toll-like receptor 4 agonist lipopolysaccharide (LPS) on the macrophage cell line RAW 264.7 (35% inhibition at a concentration of 100 μg/mL). These findings contribute to increase the ranking in the value-chain of Gracilaria sp. biomass cultivated under controlled conditions on IMTA systems.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Valorization of Lipids from Gracilaria sp. through Lipidomics and Decoding of Antiproliferative and Anti-Inflammatory Activity

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“…2 ) play important roles in many biological processes, [5] the overproduction of these species often contributes to the vast number of diseases such as inflammatory, cancer, atherosclerosis, diabetes mellitus, hypertension and others.…”

Section: Resultsmentioning

confidence: 99%

“…Recently, several papers focusing on the lipidomic characterization of marine resources have discussed this topic. Melo et al [5] characterized the polar lipids and fatty acid composition of macroalgae Chondrus crispus, Santos et al [6] published a detailed study about lipophilic composition of Codium tomentosum, Ulva lactuca, Gracilaria vermiculophylla and C. crispus, and Kendel et al [7] focused on the lipidomic characterization of Ulva armoricana, and Solieria chordalis seaweeds. Another very perspective group of chemicals from this point of view can be the sulfated polysaccharides (fucoidans, galactans, and ulvans) which have been revealed to possess many beneficial biological and therapeutic properties such as anticoagulant, antiviral, antioxidative, antitumor, immunomodulating, antihyperlipidemic, and antihepatotoxic.…”

Section: Introductionmentioning

confidence: 99%

Bioprospecting of Turbinaria Macroalgae as a Potential Source of Health Protective Compounds

Stranska-Zachariasova

Kurniatanty

Gbelcová

et al. 2017

Chemistry & Biodiversity

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The present study aims to focus on the bioprospecting of marine macroalgae of Turbinaria species, plenteous biomass of the world ocean. Three types of solvents, i.e., H 2 O, MeOH/H 2 O (80:20, v/v) and hexane/i-PrOH (50:50, v/v), were used for extraction. Both the biological activity and the pattern of present chemicals were characterized. For the cell proliferation assay, the human embryonic kidney 293 cells, cervix/breast/pancreatic adenocarcinoma, and osteosarcoma cells were used. For the antioxidant activity determination, both intracellular assay with human embryonic kidney and cervix adenocarcinoma cells, as well as the biochemical DPPH test, were employed. To complete the information about macroalgae composition, organic compounds were characterized by the liquid chromatography coupled with high resolution tandem mass spectrometry. Attention was concentrated mainly on the lipidomic profile characterization. In spite the fact that any significant antiproliferative effect was not observed for cancer cells, both the Turbinaria species were shown to be good protectors against the oxidative stress of the noncancer cells. Most of the antioxidants were determined in the hexane/i-PrOH extract. As regards the lipids identified, most of them belonged to the triacylglycerols followed by sphingomyelins, diacylglycerols, and polar (lyso) phospholipids. Additionally to fatty acids with 14, 16 and 18 carbons, also those with odd carbon numbers were frequently present.

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Mapping the Polar Lipidome of Macroalgae using LC‐MS‐Based Approaches for Add‐Value Applications

Lopes

Rey

Melo

et al. 2023

Euro J Lipid Sci & Tech

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Marine macroalgae are emerging as sustainable and healthy food, and a source of lipids with nutritional and bioactive properties. Nonetheless, the lipidome of macroalgae is little explored compared to other photosynthetic organisms. Most of the literature is devoted to the analysis of fatty acid (FA) from macroalgae, because of they are a source of long-chain omega-3 and omega-6 polyunsaturated FA, which can be used as an alternative to fish oil. However, FA occurs mostly esterified in other lipids, such as polar lipids, that still overlooked. The data published on the study of polar lipids (phospholipids, glycolipids, betaine lipids) pinpoint their remarkable characteristics and bioactive properties which mainly remain unexplored. The lipidome of macroalgae seems to be species-specific, while some lipid classes appear to be characteristic of phyla. Lipidome plasticity of macroalgae is verified to change under abiotic and biotic factors which may modulate the functionality and add-value of the biomass. This biochemical plasticity represents an advantage to obtain ingredients for target applications and niche of markets. This review provides an overview on macroalgae lipidome characterization, addressing analysis workflow based on liquid chromatography coupled to mass spectrometry, and discussing the bioactive properties and biotechnological applications of macroalga lipids. Practical Applications: The current state of the art on marine macroalgae lipidomics is reviewed, including the lipid characterization using modern liquid chromatography-mass spectrometry, the distribution of polar lipids by macroalgae species and phylum, their bioactive properties, and potential biotechnological applications. In a time of increasing awareness of sustainably produced marine resources, the lipids from macroalgae are still underexplored. This review provides information for smart valorization of macroalgae as source of add-value lipids contributing to the development of blue bioeconomy.

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Lipidomics as a new approach for the bioprospecting of marine macroalgae — Unraveling the polar lipid and fatty acid composition of Chondrus crispus

Cited by 88 publications

References 71 publications

Valorization of Lipids from Gracilaria sp. through Lipidomics and Decoding of Antiproliferative and Anti-Inflammatory Activity

Valorization of Lipids from Gracilaria sp. through Lipidomics and Decoding of Antiproliferative and Anti-Inflammatory Activity

Bioprospecting of Turbinaria Macroalgae as a Potential Source of Health Protective Compounds

Mapping the Polar Lipidome of Macroalgae using LC‐MS‐Based Approaches for Add‐Value Applications

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