Lipidomic Profiling of Liver Tissue from Obesity-Prone and Obesity-Resistant Mice Fed a High Fat Diet

Nam, Miso; Choi, Myung‐Sook; Jung, Sunhee; Jung, Youngae; Choi, Jiyoung; Ryu, Do Hyun; Hwang, Geum‐Sook

doi:10.1038/srep16984

Cited by 60 publications

(52 citation statements)

References 50 publications

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“…; Nam et al . ). The lipophilic dye Nile Red, which becomes fluorescent in hydrophobic environments, has been used to monitor lipid content in liver and primary liver cells (Li et al .…”

Section: Resultsmentioning

confidence: 97%

“…Exposure to HF diet and obesity change the phospholipid composition of the liver in rodents and induce a rise of PC/PE ratio resulting in endoplasmic reticulum stress and hepatocyte injury (Nam et al . ; Sanyal and Pacana ) (Fu et al . ).…”

Section: Resultsmentioning

confidence: 99%

“…; Nam et al . ; Sanyal and Pacana ; Engin ), virtually none of these changes are detectable in the hypothalamus. In the liver of obese mice, increased PC/PE ratio induces endoplasmic stress and injury (Fu et al .…”

Section: Discussionmentioning

confidence: 97%

“…; Nam et al . ). In humans, most of the triacylglycerol that accumulate in the liver of obese individuals is contributed by circulating FFA (Donnelly et al .…”

Section: Discussionmentioning

confidence: 97%

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Injury to hypothalamic Sim1 neurons is a common feature of obesity by exposure to high‐fat diet in male and female mice

Nyamugenda

Trentzsch

Russell

et al. 2019

Journal of Neurochemistry

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The hypothalamus is essential for regulation of energy homeostasis and metabolism. Feeding hypercaloric, high‐fat (HF) diet induces hypothalamic arcuate nucleus injury and alters metabolism more severely in male than in female mice. The site(s) and extent of hypothalamic injury in male and female mice are not completely understood. In the paraventricular nucleus (PVN) of the hypothalamus, single‐minded family basic helix‐loop helix transcription factor 1 (Sim1) neurons are essential to control energy homeostasis. We tested the hypothesis that exposure to HF diet induces injury to Sim1 neurons in the PVN of male and female mice. Mice expressing membrane‐bound enhanced green fluorescent protein (mEGFP) in Sim1 neurons (Sim1‐Cre:Rosa‐mEGFP mice) were generated to visualize the effects of exposure to HF diet on these neurons. Male and female Sim1‐Cre:Rosa‐mEGFP mice exposed to HF diet had increased weight, hyperleptinemia, and developed hepatosteatosis. In male and female mice exposed to HF diet, expression of mEGFP was reduced by > 40% in Sim1 neurons of the PVN, an effect paralleled by cell apoptosis and neuronal loss, but not by microgliosis. In the arcuate nucleus of the Sim1‐Cre:Rosa‐mEGFP male mice, there was decreased alpha‐melanocyte‐stimulating hormone in proopiomelanocortin neurons projecting to the PVN, with increased cell apoptosis, neuronal loss, and microgliosis. These defects were undetectable in the arcuate nucleus of female mice exposed to the HF diet. Thus, injury to Sim1 neurons of the PVN is a shared feature of exposure to HF diet in mice of both sexes, while injury to proopiomelanocortin neurons in arcuate nucleus is specific to male mice. Open Science Badges This article has received a badge for *Open Materials* because it provided all relevant information to reproduce the study in the manuscript. The complete Open Science Disclosure form for this article can be found at the end of the article. More information about the Open Practices badges can be found at https://cos.io/our-services/open-science-badges/.

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“…; Nam et al . ). The lipophilic dye Nile Red, which becomes fluorescent in hydrophobic environments, has been used to monitor lipid content in liver and primary liver cells (Li et al .…”

Section: Resultsmentioning

confidence: 97%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 97%

“…; Nam et al . ). In humans, most of the triacylglycerol that accumulate in the liver of obese individuals is contributed by circulating FFA (Donnelly et al .…”

Section: Discussionmentioning

confidence: 97%

See 2 more Smart Citations

Injury to hypothalamic Sim1 neurons is a common feature of obesity by exposure to high‐fat diet in male and female mice

Nyamugenda

Trentzsch

Russell

et al. 2019

Journal of Neurochemistry

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“…Many studies have investigated the lipid content in a liver tissue of NASH or NAFLD (Byeon, et al, 2016;García-Cañaveras, et al, 2011;Eisinger, et al, 2014;Gorden, et al, 2015;Nam, et al, 2015). Most of the previous studies on hepatic fat focused on triglycerides (TG) metabolism, because TG is the major constituent of hepatic fat.…”

Section: Analysis Of Lpe Regioisomers By Lc/msmentioning

confidence: 99%

Rapid tin-mediated access to a lysophosphatidylethanolamine (LPE) library: Application to positional LC/MS analysis for hepatic LPEs in non-alcoholic steatohepatitis model mice

Furukawa

Fuda

Miyanaga

et al. 2016

Chemistry and Physics of Lipids

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Abstract. Even though lysophospholipids have attracted much interest in recent years on account of their unique bioactivity, research related to lysophospholipids is usually hampered by problems associated with standard sample preparation and discrimination of regioisomers. Herein, we demonstrate a quick tin-chemistry-based synthetic route to lysophosphatidylethanolamines (LPEs) and its application in the positional analysis of hepatic LPEs in non-alcoholic steatohepatitis (NASH) model mice. We found that the preference of hepatic LPE regioisomer largely depends on the unsaturation of acyl chain in both control and NASH model mice. In addition, hepatic C18:2-LPE and C20:5-LPE levels were significantly lower in the NASH model mice than those in the control. The LC/MS technique based on the library of LPE regioisomers allows an accurate observation of hepatic LPE metabolism and might provide useful information to elucidate yet ambiguous pathogenesis of NASH.2

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Muscular lipidomics and transcriptomics reveal the effects of bile acids on lipid metabolism in high-fat diet-fed grouper

Shi

Chen

et al. 2023

Fish Physiol Biochem

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Lipidomic Profiling of Liver Tissue from Obesity-Prone and Obesity-Resistant Mice Fed a High Fat Diet

Cited by 60 publications

References 50 publications

Injury to hypothalamic Sim1 neurons is a common feature of obesity by exposure to high‐fat diet in male and female mice

Injury to hypothalamic Sim1 neurons is a common feature of obesity by exposure to high‐fat diet in male and female mice

Rapid tin-mediated access to a lysophosphatidylethanolamine (LPE) library: Application to positional LC/MS analysis for hepatic LPEs in non-alcoholic steatohepatitis model mice

Muscular lipidomics and transcriptomics reveal the effects of bile acids on lipid metabolism in high-fat diet-fed grouper

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