Lipidomic characterization of exosomes isolated from human plasma using various mass spectrometry techniques

Peterka, Ondřej; Jirásko, Robert; Chocholoušková, Michaela; Kuchař, Ladislav; Wolrab, Denise; Hájek, Roman; Vrána, David; Strouhal, Ondřej; Melichar, Bohuslav; Holčapek, Michal

doi:10.1016/j.bbalip.2020.158634

Cited by 36 publications

(36 citation statements)

References 41 publications

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“…The lipidic composition of exosomes derived from different sources and the enrichment of some lipid classes concerning producer cells has been extensively reported in several works 17,22,23 . In this sense, B‐lymphocyte‐derived exosomes are enriched in cholesterol (CHOL) up to 3 times more when compared to the cell membrane 24 .…”

Section: Exosome Lipid Compositionmentioning

confidence: 96%

“…In exosome lipidomics, hyphenated micro‐LC‐Q‐TOF‐MS, as well as nanoflow ultrahigh‐performance liquid chromatography (UHPLC)‐MS/MS systems, have been used to study lipid alterations in exosomes from both urine samples and cell culture media, respectively, obtaining high‐quality results 99,100 . Furthermore, UHPLC‐MS and ultrahigh‐performance supercritical fluid chromatography (UHPSFC)‐MS provided high‐separation efficiency and short analysis times in a lipidomic study of human plasma‐derived exosomes 23 . In this line, it was reported that using ammonium acetate and ammonium formate without acidifiers as mobile‐phase modifier systems in ESI (+) and ESI (−) modes, respectively, of UHPLC‐MS, increased the lipidome coverage in both lipid standard mixtures as well as in blood plasma lipids 101 .…”

Section: Novel Analytical Procedures For Exosomal Lipid Analysismentioning

confidence: 99%

“…21 The lipidic composition of exosomes derived from different sources and the enrichment of some lipid classes concerning producer cells has been extensively reported in several works. 17,22,23 In this sense, B-lymphocyte-derived exosomes are enriched in cholesterol (CHOL) up to 3 times more when compared to the cell membrane. 24 Apparently, CHOL starts to be accumulated in MVBs and this process appears to be essential for the formation of intraluminal vesicles, the precursors of exosomes.…”

Section: Exosome Lipid Compositionmentioning

confidence: 99%

“… 99 , 100 Furthermore, UHPLC‐MS and ultrahigh‐performance supercritical fluid chromatography (UHPSFC)‐MS provided high‐separation efficiency and short analysis times in a lipidomic study of human plasma‐derived exosomes. 23 In this line, it was reported that using ammonium acetate and ammonium formate without acidifiers as mobile‐phase modifier systems in ESI (+) and ESI (−) modes, respectively, of UHPLC‐MS, increased the lipidome coverage in both lipid standard mixtures as well as in blood plasma lipids. 101 Besides, the use of in silico simulations of data acquisition performance has been proposed to identify the optimal method parameters in LC‐MS/MS for lipidomic research, considering the synergistic relationship between MS method parameters and avoiding sub‐optimal results.…”

Section: Novel Analytical Procedures For Exosomal Lipid Analysismentioning

confidence: 99%

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The role of lipids in exosome biology and intercellular communication: Function, analytics and applications

Donoso‐Quezada

Ayala‐Mar

González‐Valdez

2021

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Exosomes are extracellular vesicles that in recent years have received special attention for their regulatory functions in numerous biological processes. Recent evidence suggests a correlation between the composition of exosomes in body fluids and the progression of some disorders, such as cancer, diabetes and neurodegenerative diseases. In consequence, numerous studies have been performed to evaluate the composition of these vesicles, aiming to develop new biomarkers for diagnosis and to find novel therapeutic targets. On their part, lipids represent one of the most important components of exosomes, with important structural and regulatory functions during exosome biogenesis, release, targeting and cellular uptake. Therefore, exosome lipidomics has emerged as an innovative discipline for the discovery of novel lipid species with biomedical applications. This review summarizes the current knowledge about exosome lipids and their roles in exosome biology and intercellular communication. Furthermore, it presents the state‐of‐the‐art analytical procedures used in exosome lipidomics while emphasizing how this emerging discipline is providing new insights for future applications of exosome lipids in biomedicine.

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Section: Exosome Lipid Compositionmentioning

confidence: 96%

Section: Novel Analytical Procedures For Exosomal Lipid Analysismentioning

confidence: 99%

Section: Exosome Lipid Compositionmentioning

confidence: 99%

Section: Novel Analytical Procedures For Exosomal Lipid Analysismentioning

confidence: 99%

See 2 more Smart Citations

The role of lipids in exosome biology and intercellular communication: Function, analytics and applications

Donoso‐Quezada

Ayala‐Mar

González‐Valdez

2021

Traffic

131

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“…Molecular lipid species, including glycerolipids, phospholipids, sphingolipids, and ceramides are involved in various biological functions including energy storage, serving as precursors for metabolic processes and as main components of cellular building blocks [ 8 ]. Perturbations in lipid metabolism can lead to lipotoxicity, generally defined as an increased concentration of detrimental lipids such as specific lipids with saturated fatty acyls and ceramides [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Effects of a Lacto-Ovo-Vegetarian Diet on the Plasma Lipidome and Its Association with Atherosclerotic Burden in Patients with Coronary Artery Disease—A Randomized, Open-Label, Cross-over Study

et al. 2020

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A vegetarian diet has been associated with a lower risk of coronary artery disease (CAD). Plasma triacylglycerols, ceramides, and phosphatidylcholines may improve prediction of recurrent coronary events. We sought to investigate effects of a lacto-ovo-vegetarian diet (VD) on plasma lipidome in CAD patients and simultaneously assess associations of plasma lipids with the extent of coronary atherosclerotic burden. We analyzed 214 plasma lipids within glycerolipid, sphingolipid, and sterol lipid classes using lipidomics from a randomized controlled, crossover trial comprising 31 CAD patients on standard medical therapy. Subjects completed a four-week intervention with VD and isocaloric meat diet (MD), separated by a four-week washout period. The VD increased levels of 11 triacylglycerols and lowered 7 triacylglycerols, 21 glycerophospholipids, cholesteryl ester (18:0), and ceramide (d18:1/16:0) compared with MD. VD increased triacylglycerols with long-chain polyunsaturated fatty acyls while decreased triacylglycerols with saturated fatty acyls, phosphatidylcholines, and sphingomyelins than MD. The Sullivan extent score (SES) exhibited on coronary angiograms were inversely associated with triacylglycerols with long-chain polyunsaturated fatty acyls. Phosphatidylcholines that were lower with VD were positively associated with SES and the total number of stenotic lesions. The VD favorably changed levels of several lipotoxic lipids that have previously been associated with increased risk of coronary events in CAD patients.

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Microfluidic Chips Fabrication and Integration for Tumor‐Derived Exosomes Detection

Sun

Gao

et al. 2023

Adv Materials Technologies

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Exosomes are a type of small extracellular vesicles carrying proteins, nucleic acids, and lipids. Because their expression levels are highly correlated with tumor development, exosomes are regarded as non‐invasive biomarkers for cancer diagnosis. Therefore, many exosome detection techniques are developed to provide a new way for cancer diagnosis. However, conventional techniques require the isolation of exosomes from body fluids before analysis, which usually relies on complex equipment and cumbersome operations. In addition, complex procedures may introduce impurities and thus affect the accuracy of the test. As an emerging analysis technology, microfluidic overcomes these shortcomings by integrating a microchannel for sample transport isolation and an exosome analysis module, showing great prospects in cancer diagnosis. This review will provide an overview of microchannel fabrication and analysis methods with outstanding compatibility for different microfluidic types, with a particular focus on microfluidic‐based exosome analysis methods that have the potential for clinical application.

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Lipidomic characterization of exosomes isolated from human plasma using various mass spectrometry techniques

Cited by 36 publications

References 41 publications

The role of lipids in exosome biology and intercellular communication: Function, analytics and applications

The role of lipids in exosome biology and intercellular communication: Function, analytics and applications

Effects of a Lacto-Ovo-Vegetarian Diet on the Plasma Lipidome and Its Association with Atherosclerotic Burden in Patients with Coronary Artery Disease—A Randomized, Open-Label, Cross-over Study

Microfluidic Chips Fabrication and Integration for Tumor‐Derived Exosomes Detection

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