Lipidomic Analysis of Glycerolipid and Cholesteryl Ester Autooxidation Products

Abstract

Thin-layer chromatography (TLC), gas chromatography (GC), and liquid chromatography (LC) in combination with mass spectrometry (MS) have been adopted for the isolation and identification of oxolipids and for determining their functionality. TLC provides a rapid separation and access to most oxolipids as intact molecules and has recently been effectively interfaced with time-of-flight (TOF) MS (TOF-MS). GC with flame ionization (FI) (GC/FI) and electron impact (EI) MS (GC/EI-MS) has been extensively utilized in the analysis of isoprostanes and other low-molecular-weight oxolipids, although these methods require derivatization of the analytes. In contrast, LC with ultraviolet (UV) absorption (LC/UV) or evaporate light scattering detection (ELSD) (LC/ELSD) as well as electrospray ionization (ESI) or atmospheric pressure chemical ionization (APCI) MS (LC/ESI-MS) or LC/APCI-MS has proven to be well suited for the analysis of intact oxolipids and their conjugates without or with minimal derivatization. Nevertheless, kit-based colorimetric and fluorescent procedures continue to serve as sensitive indicators of the presence of hydroperoxides and aldehydes.

Cholesterol and fatty acids regulate cysteine ubiquitylation of ACAT2 through competitive oxidation

“…After vo r tex and centrifugation, the upper organic phase was collected, mixed with hexane and extract again. All organic phase was dried under argon and reconstituted in hexane prior to injection 46 .…”

Quantitative determination of major oxidation products in edible oils by direct NP-HPLC-DAD analysis

“…Isolation and structural determination of the main oxidation products of the common dietary unsaturated fatty acids were well established using model lipid systems at the end of the 70's [12]. Complex analytical methods mainly based on the combination of liquid chromatography and mass spectrometry have been reported for the identification of oxidation compounds in a variety of samples, principally those of biological nature [13][14][15]. Recently, chromatography methods for the analyses of primary [16] and secondary [17] oxidation products of triacylglycerols (TAG) have been reviewed.…”

Antioxidative properties of fish roe peptides combined with polyphenol on the fish oil oleogel

“…Specifically, while the trans double bonds continue to increase, the cis double bonds are also decreasing. Because the characteristic peak of the υ (CC) cis double bond is located at 1656 cm −1 in the Raman spectrum, the 1746 cm −1 peak reflecting the ester bond, which is more similar, was selected as a reference and changes in unsaturation with accelerated storage time were characterized by I (1656) / I (1746) 39 . As shown in Fig.…”