Lipid transfer proteins are encoded by a small multigene family in Arabidopsis thaliana

Arondel, Vincent; Vergnolle, Chantal; Cantrel, Catherine; Kader, Jean‐Claude

doi:10.1016/s0168-9452(00)00232-6

Cited by 190 publications

(150 citation statements)

References 39 publications

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“…They form a multigene family encoding 9-kDa proteins (90-95 amino acids) that are distributed throughout the plant kingdom. In Arabidopsis, at least six individual nsLTP genes have been found on three chromosomes (Arondel et al 2000). Kader (1996) suggested that nsLTPs are pathogenesis-related since they can participate in plant defense reactions through anti-fungal and anti-bacterial activities.…”

Section: Communicated By R Hagemannmentioning

confidence: 99%

Linkage map positions and allelic diversity of two Mal d 3 (non-specific lipid transfer protein) genes in the cultivated apple (Malus domestica)

et al. 2005

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Non-specific lipid transfer proteins (nsLTPs) of Rosaceae fruits, such as peach, apricot, cherry, plum and apple, represent major allergens for Mediterranean atopic populations. As a first step in elucidating the genetics of nsLTPs, we directed the research reported here towards identifying the number and location of nsLTP (Mal d 3) genes in the apple genome and determining their allelic diversity. PCR cloning was initially performed on two cultivars, Prima and Fiesta, parents of a core apple mapping progeny in Europe, based on two Mal d 3 sequences (AF221502 and AJ277164) in the GenBank. This resulted in the identification of two distinct sequences (representing two genes) encoding the mature nsLTP proteins. One is identical to accession AF221502 and has been named Mal d 3.01, and the other is new and has been named Mal d 3.02. Subsequent genome walking in the upstream direction and DNA polymorphism analysis revealed that these two genes are intronless and that they could be mapped on two homoeologous segments of linkage groups 12 and 4, respectively. Further cloning and sequencing of the coding and upstream region of both Mal d 3 genes in eight cultivars was performed to identify allelic variation. Assessment of the deduced nsLTP amino acid sequences gave a total of two variants at the protein level for Mal d 3.01 and three for Mal d 3.02. The consequences of our results for allergen nomenclature and the breeding of low allergenic apple cultivars are discussed.

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Section: Communicated By R Hagemannmentioning

confidence: 99%

Linkage map positions and allelic diversity of two Mal d 3 (non-specific lipid transfer protein) genes in the cultivated apple (Malus domestica)

et al. 2005

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“…1.4 Phylogenetic tree construction and secondary structure prediction of SCA Ten amino acid sequence of Lily SCA except LaSCA2 and fifty seven known amino acid sequences of other plant LTP were employed to do alignment analysis to build the phylogenetic tree ( Figure 4). It was found that the cloned ten sequences were clustered into a group with Lilium longiflorum 'Nellie white' SCA and (Arondel et al, 2000), resulting in differences in amino acid sequence of LTP proteins. Barley LTP2, wheat LTP2-1 and wheat LTP2-2 did not come together in the grass group, but went to form a single large branch with the cowpea nsLTP, which would be the reason that LTP can be divided into LTP Ⅰand LTP Ⅱ the size of the protein molecular weight.…”

Section: Results and Analysismentioning

confidence: 99%

Cloning of SCA Gene Related to Pollen Tube Adhesion and Oriented Growth and Analysis of Gene Diversity in <i>Lilium spp.</i>

Wu¹,

Cui²,

Zhang³

et al. 2012

mpb

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Stigma/style cysteine-rich adhesin genes (SCA) were cloned from 'Tresor' (Lilium Asiatic Hybrids), 'White heaven' (Lilium Longiflorum Hybrids) and 'Caruso' (Lilium Oriental Hybrids) leaves by PCR approaches. In this research we obtained four gene copies in both of 'Tresor' and 'Caruse' genomes and three gene copies in 'White heaven' genome. The identities of the cloned SCA among the copies were in the ranges from 72.97% to 99.68%. There are two exons and one intron in all of cloned SCA sequence, which could be deduced immature protein with 113 or 115 amino acid residues (encoding mature protein with 91 amino acid residues) SCA protein contains an N-terminal signal peptide of typical LTP protein, eight conserved cysteine residues and two consensus pentapeptide motifs. The identities among the amino acid sequences were in range from 87.91% to 98.90% higher than that of the similarities of gene sequences. Alignment analysis of amino acid sequences of cloned SCAs showed there were some distinct differences of amino acid site existing in the tested three species of lily hybrids. The phylogenetic tree revealed the relationship of the cloned 11 amino acid sequences of SCA were all more close to LTP family of monocotyledons than that of dicotyledons, which were consistent with the conclusions of kinship in plant taxonomy.

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“…It is known that the LOX pathway branches immediately after the LOX reaction into several different directions to make a whole variety of compounds likely to have bioactivity [9]. It is also clear from the Arabidopsis genome that the lipid transfer protein gene family [36] in Arabidopsis has at least 25 members, all of which are apoplastic and one of which is the potential SAR mobile carrier DIR1 [35]. Thus, it is likely that plant signaling at a distance may include the regulated synthesis of specific lipids at source sites and the subsequent apoplastic delivery of those bioactive compounds to target sites.…”

Section: Discussionmentioning

confidence: 99%

The developmental transition to flowering in Arabidopsis is associated with an increase in leaf chloroplastic lipoxygenase activity

Bañuelos¹,

Argumedo²,

Patel³

et al. 2008

Plant Science

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The developmental transition from vegetative growth to flowering in Arabidopsis is associated with a precipitous decline in the activity of leaf ascorbate peroxidase (APx), an enzymatic scavenger of hydrogen peroxide, and an increase in specific lipid peroxidation leading to the accumulation of 13-hydroperoxy-9,11,15 (Z,E,Z) octadecatrienoic acid (13 HOO-FA). The appearance of this specific isomer suggests that it is of enzymatic origin and may represent the activation of an oxylipin signaling pathway. We thus hypothesized that leaf 13-lipoxygenase (LOX) activity increases at the floral transition and leads to the observed elevation of 13-HOO-FA levels. Leaf protein extracts were prepared from seven distinct life stages of Arabidopsis plants and used to assay for LOX activity. We report that leaf 13-LOX enzymatic activity increases twoto three-fold from the vegetative stage to the immediate post-floral transition stage. We found two forms of LOX activity in cell extracts and show that the higher pH optimum form is the isoenzyme activated. This increase is correlated with a small increase in H 2 O 2 , perhaps resulting from the previously reported decline in leaf APx activity. Very low levels of exogenous H 2 O 2 activate the induced form in vegetative leaf extracts in vitro, suggesting that the floral transition-dependent APx decline and subsequent H 2 O 2 elevation are involved in activating plastid 13-LOX and thus a second messenger oxylipin pathway.

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Lipid transfer proteins are encoded by a small multigene family in Arabidopsis thaliana

Cited by 190 publications

References 39 publications

Linkage map positions and allelic diversity of two Mal d 3 (non-specific lipid transfer protein) genes in the cultivated apple (Malus domestica)

Linkage map positions and allelic diversity of two Mal d 3 (non-specific lipid transfer protein) genes in the cultivated apple (Malus domestica)

Cloning of SCA Gene Related to Pollen Tube Adhesion and Oriented Growth and Analysis of Gene Diversity in <i>Lilium spp.</i>

The developmental transition to flowering in Arabidopsis is associated with an increase in leaf chloroplastic lipoxygenase activity

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