Lipid transfer protein from Manducasexta hemolymph

Ryan, Robert O.; Wells, Michael A.; Law, John H.

doi:10.1016/0006-291x(86)90903-4

Cited by 65 publications

(12 citation statements)

References 8 publications

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“…The carbohydrate contents of housefly lipophorin (3.9%) are similar to those described for Locustu rnigrutoria [2]. Covalently bound mannose-con- [26,27]. The density of housefly lipophorin (1.106 glml) is lower than that reported for Surcophugu bullutu (1.12 glml) [6], the only density value for adult dipteran lipophorin reported until now.…”

Section: Discussionsupporting

confidence: 52%

Lipophorin in the larval and adult stages of Musca domestica

Bianchi

Capurro

Marinotti

1987

Arch Insect Biochem Physiol

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The major Mosca domestica hemolymph lipoprotein, lipophorin, was purified from larval and from adult animals. The housefly lipophorin is composed of two apoproteins, apolipophorin I (M, -253,000) and apolipophorin II (M, -85,000). The lipophorin contains about 3.9% carbohydrates and reacts positively with concanavalin A. The density of larval lipophorin is equal to 1.152 glml and of adult lipophorin to 1.106 glml. The amount of lipophorin per animal increases during the larval stage, is constant during pupal stage, and suffers a great reduction at the pharate adult stage. The amount of lipophorin remains stable during the whole first gonotrophic cycle of the housefly. Lipophorin is not detected in the eggs of this insect.

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Section: Discussionsupporting

confidence: 52%

Lipophorin in the larval and adult stages of Musca domestica

Bianchi

Capurro

Marinotti

1987

Arch Insect Biochem Physiol

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“…LTP facilitates the exchange and net transfer of different lipids between lipophorins (Ryan et al, 1986a(Ryan et al, , 1988Tsuchida et al, 1997;Takeuchi and Chino, 1993;Tsuchida et al, 1998). In addition, LTP promotes the net transfer of lipids from the fat body of adult M. sexta (Van Heusden and Law, 1989) and from larval midgut Arrese et al, 2001) to lipophorin.…”

Section: Ltp-mediated Lipid Deliverymentioning

confidence: 93%

Lipid uptake by insect oocytes

Ziegler

Antwerpen

2006

Insect Biochemistry and Molecular Biology

199

172

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“…sexta LDLp contains 62% lipid [El. There are indications that a hemolymph LTP may be involved in lipid loading of HDLp [14,and Van Heusden,unpublished data).…”

Section: Introductionmentioning

confidence: 91%

“…An LTP has been suggested to function in the loading of lipids onto lipophorin in M. sextu [14]. Since such a catalyst could be lacking in B. psolus, LTP isolated from M. sextu was injected into B. psolus followed by injection of AKH.…”

Section: Possible Role Of Lipid Transfer Particlementioning

confidence: 99%

Adipokinetic response of a flightless grasshopper (Barytettix psolus): Functional components, defective response

Ziegler

Ryan

Arbas

et al. 1988

Arch Insect Biochem Physiol

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The mature flightless grasshopper Barytettix psolus shows a very small adipokinetic response when injected with extracts of its own corpora cardiaca, although the fat body contains enough lipid for a strong response. When these extracts were injected into Melanoplus differentialis, a grasshopper capable of flight, or the moth Manduca sexta, much stronger adipokinetic responses were observed. Upon analysis of B. psolus extracts by HPLC, two components with adipokinetic activity were obtained. The major component appears to be identical to locust adipokinetic hormone (AKH) I. Extracts of B. psolus corpora cardiaca also activated fat body glycogen phosphorylase in B. psolus. This activation, however, did not result in an increase in hemolymph sugar, probably because of low levels of glycogen in the fat body.8. psoius hemolymph contains a high-density lipophorin (HDLp) consisting of the apolipophorins (apoLp) I and II and lipid. Both apoproteins are glycosylated. The hemolymph also contains apoLp-Ill, although this apoprotein apparently does not associate with HDLp to form a low-density lipophorin (LDLp) following AKH or corpora cardiaca extract injections. When B. psolus lipophorin and AKH were injected into Schisfocerca americana, lipophorin took up lipids and combined with apoLp-Ill, forming LDLp. ApoLp-Ill from B.psolus injected into S. americana can also form LDLp, demonstrating that the components are functional. A lipid transfer particle isolated from M. sexta and injected into 5. psolus does not improve the adipokinetic response.Thus, it appears that the adipokinetic response of B. psolus is not deficient because of the lack of AKH or functional lipophorins, but may be caused by the lack of a full response to AKH by fat body or the deficiency in hemolymph of some as yet unknown factor. Acknowledgments: The authors thank Dr. D.J. Van der Horst for a generous gift of L. migratoria apoLp-Ill.

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Lipid transfer protein from Manducasexta hemolymph

Cited by 65 publications

References 8 publications

Lipophorin in the larval and adult stages of Musca domestica

Lipophorin in the larval and adult stages of Musca domestica

Lipid uptake by insect oocytes

Adipokinetic response of a flightless grasshopper (Barytettix psolus): Functional components, defective response

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