Lipid transfer particle from the silkworm, Bombyx mori, is a novel member of the apoB/large lipid transfer protein family

Yokoyama, Hiroshi; Yokoyama, Takeru; Yuasa, Masashi; Fujimoto, Haruhiro; Sakudoh, Takashi; Honda, Naoko; Fugo, Hajime; Tsuchida, Kozo

doi:10.1194/jlr.m037093

Cited by 15 publications

(11 citation statements)

References 50 publications

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“…LTP is a highly active and unique lipid transfer protein that is found in the hemolymph of insects, and that catalyzes the bidirectional lipid transfer/exchange between lipoproteins and tissues (Canavoso and Wells, 2001; Liu and Ryan, 1991; Van Heusden and Law, 1989), and between lipoproteins (Ryan et al, 1988; Tsuchida et al, 1997). The B. mori LTP gene was recently identified and characterized (Yokoyama et al, 2013). BmLTP has three subunits.…”

Section: Resultsmentioning

confidence: 99%

“…BmLTP has three subunits. Two of them, apoLTP-I and II, are encoded by a single gene (4121 amino acids), whereas the third subunit is encoded by a different gene (Yokoyama et al, 2013). We confirmed that Msex2.09991 encodes the two major subunits of LTP, LTP-I & -II.…”

Section: Resultsmentioning

confidence: 99%

“…The tree emphasizes the comparison of LTP protein sequences, which seem to be unique to insects. In addition to the LTP sequences previously reported from B. mori and D. melanogaster (Yokoyama et al, 2013), the tree includes the predicted LTP sequences from eight more insect species. As previously inferred for BmLTP (Yokoyama et al, 2013), LTPs seem to share a common ancestor with apoLp-I&II and even human apoB.…”

Section: Resultsmentioning

confidence: 99%

“…In addition to the LTP sequences previously reported from B. mori and D. melanogaster (Yokoyama et al, 2013), the tree includes the predicted LTP sequences from eight more insect species. As previously inferred for BmLTP (Yokoyama et al, 2013), LTPs seem to share a common ancestor with apoLp-I&II and even human apoB. The process of secretion of DG may also require the specific interaction of lipophorin with a lipophorin receptor (Lp-R).…”

Section: Resultsmentioning

confidence: 99%

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Multifaceted biological insights from a draft genome sequence of the tobacco hornworm moth, Manduca sexta

Kanost

Arrese

Cao

et al. 2016

Insect Biochemistry and Molecular Biology

161

174

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Manduca sexta, known as the tobacco hornworm or Carolina sphinx moth, is a lepidopteran insect that is used extensively as a model system for research in insect biochemistry, physiology, neurobiology, development, and immunity. One important benefit of this species as an experimental model is its extremely large size, reaching more than 10 g in the larval stage. M. sexta larvae feed on solanaceous plants and thus must tolerate a substantial challenge from plant allelochemicals, including nicotine. We report the sequence and annotation of the M. sexta genome, and a survey of gene expression in various tissues and developmental stages. The Msex_1.0 genome assembly resulted in a total genome size of 419.4 Mbp. Repetitive sequences accounted for 25.8% of the assembled genome. The official gene set is comprised of 15,451 protein-coding genes, of which 2498 were manually curated. Extensive RNA-seq data from many tissues and developmental stages were used to improve gene models and for insights into gene expression patterns. Genome wide synteny analysis indicated a high level of macrosynteny in the Lepidoptera. Annotation and analyses were carried out for gene families involved in a wide spectrum of biological processes, including apoptosis, vacuole sorting, growth and development, structures of exoskeleton, egg shells, and muscle, vision, chemosensation, ion channels, signal transduction, neuropeptide signaling, neurotransmitter synthesis and transport, nicotine tolerance, lipid metabolism, and immunity. This genome sequence, annotation, and analysis provide an important new resource from a well-studied model insect species and will facilitate further biochemical and mechanistic experimental studies of many biological systems in insects.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Multifaceted biological insights from a draft genome sequence of the tobacco hornworm moth, Manduca sexta

Kanost

Arrese

Cao

et al. 2016

Insect Biochemistry and Molecular Biology

161

174

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“…For larval spotting, we included genes in the melanin synthesis pathway and genes that have been implicated in pigmentation patterning (Wittkopp et al 2003;Protas and Patel 2008;Wittkopp and Beldade 2009;Sugumaran and Barek 2016). For larval body color, we included genes implicated in the transport, deposition, and processing of carotenoid pigments derived from the diet (Palm et al 2012;Yokoyama et al 2013;Tsuchida and Sakudoh 2015;Toews et al 2017). Although several pigments can produce yellow coloration in insects (e.g., melanins, pterins, ommochromes, and carotenoids), we focused on carotenoids because a heated pyridine test (McGraw et al 2005) was consistent with carotenoid-based coloration in N. lecontei larvae ( Figure S1 in File S1).…”

Section: Candidate Gene Analysismentioning

confidence: 99%

Genetic Basis of Body Color and Spotting Pattern in Redheaded Pine Sawfly Larvae (Neodiprion lecontei)

et al. 2018

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Pigmentation has emerged as a premier model for understanding the genetic basis of phenotypic evolution, and a growing catalog of color loci is starting to reveal biases in the mutations, genes, and genetic architectures underlying color variation in the wild. However, existing studies have sampled a limited subset of taxa, color traits, and developmental stages. To expand the existing sample of color loci, we performed QTL mapping analyses on two types of larval pigmentation traits that vary among populations of the redheaded pine sawfly (): carotenoid-based yellow body color and melanin-based spotting pattern. For both traits, our QTL models explained a substantial proportion of phenotypic variation and suggested a genetic architecture that is neither monogenic nor highly polygenic. Additionally, we used our linkage map to anchor the current genome assembly. With these data, we identified promising candidate genes underlying (1) a loss of yellow pigmentation in populations in the mid-Atlantic/northeastern United States [C locus-associated membrane protein homologous to a mammalian HDL receptor-2 gene () and lipid transfer particle apolipoproteins II and I gene ()], and (2) a pronounced reduction in black spotting in Great Lakes populations [members of the gene family, tyrosine hydroxylase gene (), and dopamine -acetyltransferase gene ()]. Several of these genes also contribute to color variation in other wild and domesticated taxa. Overall, our findings are consistent with the hypothesis that predictable genes of large effect contribute to color evolution in nature.

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Lipids in Insect Reproduction: Where, How, and Why

Leyria,

Fruttero,

Canavoso

2024

Advances in Experimental Medicine and Biology

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Lipid transfer particle from the silkworm, Bombyx mori, is a novel member of the apoB/large lipid transfer protein family

Cited by 15 publications

References 50 publications

Multifaceted biological insights from a draft genome sequence of the tobacco hornworm moth, Manduca sexta

Multifaceted biological insights from a draft genome sequence of the tobacco hornworm moth, Manduca sexta

Genetic Basis of Body Color and Spotting Pattern in Redheaded Pine Sawfly Larvae (Neodiprion lecontei)

Lipids in Insect Reproduction: Where, How, and Why

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