Lipid spin labels in lecithin multilayers. A study of motion along fatty acid chains

Jost, Patricia C.; Libertini, Louis J.; Hebert, Virgil C.; Griffith, O. Hayes

doi:10.1016/0022-2836(71)90414-1

Cited by 328 publications

(122 citation statements)

References 16 publications

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“…Previous spin label studies of phospholipid bilayers have led to a different model for the chain ordering. It was found that in bilayers with saturated hydrocarbon chains (DPPC-bilayers; Hubbel-& McConnell, 1971) as well as in bilayers prepared from natural lipids (eggyolk lecithin; Jost et al 1971;McFarland & McConnell, 1972) the spin label order parameter decreases rapidly as the paramagnetic nitroxide group approaches the centre of the bilayer. This behaviour which parallels that of spin probes incorporated into soap bilayers has been referred to as 'flexibility gradient'.…”

Section: In the Intermediate Region The Unsaturated Bilayer Is Howevementioning

confidence: 99%

Deuterium magnetic resonance: theory and application to lipid membranes

Seelig

1977

Quart. Rev. Biophys.

1,256

1,041

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Proton and carbon-13 nmr spectra of unsonicated lipid bilayers and biological membranes are generally dominated by strong proton–proton and proton–carbon dipolar interactions. As a result the spectra contain a large number of overlapping resonances and are rather difficult to analyse. Nevertheless, important information on the structure and dynamic behaviour of lipid systems has been provided by these techniques (Wennerström & Lindblom, 1977).

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Section: In the Intermediate Region The Unsaturated Bilayer Is Howevementioning

confidence: 99%

Deuterium magnetic resonance: theory and application to lipid membranes

Seelig

1977

Quart. Rev. Biophys.

1,256

1,041

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“…It has been used to study the gel ~ liquid crystal phase transition of dipalmitoyl lecithin in H20, since it has a high solubility in the fluid liquid crystal, and a low solubility in the gel [30] have been used to study the rates and types of motion at different regions along the hydrocarbon chains in bilayers in both liposome dispersions [30, 35,36] and in oriented systems [37]. When spin labelled fatty acids or lecithins are incorporated into liquid crystalline egg lecithin bilayers, it is found that the decrease in order parameters S (defined as = 0 for an isotropic liquid and = 1 for a perfect crystalline solid) is greater than logarithmic with increasing n, the number of methylene groups separating the label from the polar headgroup [30].…”

Section: Electron Spin Resonance Studiesmentioning

confidence: 99%

Dynamics of lipids in membranes: Heterogeneity and the role of cholesterol

1972

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“…The n-doxyl stearic acid (n-DSA) chains have been shown to orient themselves approximately in the direction of the liposome surface normal and parallel to the phospholipid molecules [33].…”

Section: Discussionmentioning

confidence: 99%

“…The liposoluble n-doxyl-stearic acid spin labels consist in carboxyl group bearing a skirt stearic acid chain to which a nitroxide moiety is attached [25]. In membrane, probe's carboxyl group positions itself near phospholipids polar head, whereas its stearic acid chain plunges in the membrane parallel to phospholipids chains [33]. Nitroxide moiety is located at the position n = 5 or n = 16 of the stearic acid chain [25].…”

Section: Neuro-2a Membranementioning

confidence: 99%