Lipid signalling mediated by PLD/PA modulates proline and H2O2 levels in barley seedlings exposed to short- and long-term chilling stress

Margutti, Micaela Peppino; Reyna, Matias; Meringer, María Verónica; Racagni, G.; Villasuso, Ana Laura

doi:10.1016/j.plaphy.2017.02.008

Cited by 44 publications

(22 citation statements)

References 49 publications

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“…UTEX 3007 contains several C2 calcium-binding domains, indicating that it acts on membrane phospholipids to produce membrane-bound phosphatidic acid (PA) and cytosolic choline (Rahier et al, 2016). PA has noted roles in environmental stress response (Peppino Margutti et al, 2017), and its liberation has been shown to create ‘supersized’ lipid droplets (Fei et al, 2011). We hypothesize that phospholipases acting on membrane lipids could play particularly important roles in osmotic stress resistance and lipid accumulation in Chloroidium sp.…”

Section: Resultsmentioning

confidence: 99%

The genome and phenome of the green alga Chloroidium sp. UTEX 3007 reveal adaptive traits for desert acclimatization

Nelson

Khraiwesh

et al. 2017

eLife

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To investigate the phenomic and genomic traits that allow green algae to survive in deserts, we characterized a ubiquitous species, Chloroidium sp. UTEX 3007, which we isolated from multiple locations in the United Arab Emirates (UAE). Metabolomic analyses of Chloroidium sp. UTEX 3007 indicated that the alga accumulates a broad range of carbon sources, including several desiccation tolerance-promoting sugars and unusually large stores of palmitate. Growth assays revealed capacities to grow in salinities from zero to 60 g/L and to grow heterotrophically on >40 distinct carbon sources. Assembly and annotation of genomic reads yielded a 52.5 Mbp genome with 8153 functionally annotated genes. Comparison with other sequenced green algae revealed unique protein families involved in osmotic stress tolerance and saccharide metabolism that support phenomic studies. Our results reveal the robust and flexible biology utilized by a green alga to successfully inhabit a desert coastline.DOI: http://dx.doi.org/10.7554/eLife.25783.001

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Section: Resultsmentioning

confidence: 99%

The genome and phenome of the green alga Chloroidium sp. UTEX 3007 reveal adaptive traits for desert acclimatization

Nelson

Khraiwesh

et al. 2017

eLife

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“…The involvement of the PLD gene in various biological processes including seed germination (Li et al, 2007), cold acclimation (Kargiotidou et al, 2010), resistance against pathogens (Zhao et al, 2013), and cold stress responses (Margutti et al, 2017), has been reported. In this study, the AcPLDs exhibited differential expression patterns during IB of pineapple.…”

Section: Discussionmentioning

confidence: 99%

Identification and Characterization of Phospholipase D Genes Putatively Involved in Internal Browning of Pineapple during Postharvest Storage

et al. 2017

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Phospholipase D (PLD) in plants plays vital roles in growth, development, and stress responses. However, the precise role of PLDs in pineapple remains poorly understood. In this study, 10 putative PLD genes, designated as AcPLD1–AcPLD10, were identified based on the pineapple genome database. The 10 AcPLDs could be clustered into five of the six known PLD families according to sequence characterization. Their deduced amino acid sequences displayed similarities to PLDs from other plant species. Expression analyses of PLD mRNAs from pineapple pulp were performed. The 10 PLDs exhibited differential expression patterns during storage periods of fruits treated with hexaldehyde (a specific PLD inhibitor) which could alleviate internal browning (IB) of pineapple after harvest. Functional subcellular localization signaling assays of two PLD proteins (AcPLD2 and AcPLD9) were performed by fluorescence microscopy. To further detect the potential action mechanism underlying PLD involved in the IB defense response, PLD, hydrogen peroxide (H2O2) and H2O2 associated with antioxidative enzymes such as superoxide dismutase, catalase, NADPH, and ascorbate peroxidase were quantified by enzyme-linked immunosorbent assay. This report is the first to provide a genome-wide description of the pineapple PLD gene family, and the results should expand knowledge of this family.

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“…However, despite much effort, except for the above conserved feature of cold tolerance in plants, little is known about other cold signal transduction pathways that are associated with gene expression changes. One of the latest discoveries in the field of cold signaling is the formation of the lipid second messenger, phosphatidic acid (PA), which shows to accumulate in suspension-cultured cells within minutes of cold stress (Cantrel et al, 2011;Peppino et al, 2017). In cold-induced signal transduction, PA responses have been mainly attributed to two pathways: the direct product of phospholipase D (PLD), which hydrolyses structural phospholipids such as phosphatidylcholine (PC) and phosphatidylethanolamine (PE), and the secondary product of phospholipase C (PLC) pathway, which first hydrolyzes polyphosphoinositides (PPIs) to diacylglycerol (DAG), and subsequently is phosphorylated to PA by diacylglycerol kinase (DGK) (Arisz et al, 2013;Tan et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

An Advanced Lipid Metabolism System Revealed by Transcriptomic and Lipidomic Analyses Plays a Central Role in Peanut Cold Tolerance

et al. 2020

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Cold stress restricts peanut (Arachis hypogaea L.) growth, development, and yield. However, the specific mechanism of cold tolerance in peanut remains unknown. Here, the comparative physiological, transcriptomic, and lipidomic analyses of cold tolerant variety NH5 and cold sensitive variety FH18 at different time points of cold stress were conducted to fill this gap. Transcriptomic analysis revealed lipid metabolism including membrane lipid and fatty acid metabolism may be a significant contributor in peanut cold tolerance, and 59 cold-tolerant genes involved in lipid metabolism were identified. Lipidomic data corroborated the importance of membrane lipid remodeling and fatty acid unsaturation. It indicated that photosynthetic damage, resulted from the alteration in fluidity and integrity of photosynthetic membranes under cold stress, were mainly caused by markedly decreased monogalactosyldiacylglycerol (MGDG) levels and could be relieved by increased digalactosyldiacylglycerol (DGDG) and sulfoquinovosyldiacylglycerol (SQDG) levels. The upregulation of phosphatidate phosphatase (PAP1) and phosphatidate cytidylyltransferase (CDS1) inhibited the excessive accumulation of PA, thus may prevent the peroxidation of membrane lipids. In addition, fatty acid elongation and fatty acid boxidation were also worth further studied in peanut cold tolerance. Finally, we constructed a metabolic model for the regulatory mechanism of peanut cold tolerance, in which the advanced lipid metabolism system plays a central role. This study lays the foundation for deeply analyzing the molecular mechanism and realizing the genetic improvement of peanut cold tolerance.

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Lipid signalling mediated by PLD/PA modulates proline and H2O2 levels in barley seedlings exposed to short- and long-term chilling stress

Cited by 44 publications

References 49 publications

The genome and phenome of the green alga Chloroidium sp. UTEX 3007 reveal adaptive traits for desert acclimatization

The genome and phenome of the green alga Chloroidium sp. UTEX 3007 reveal adaptive traits for desert acclimatization

Identification and Characterization of Phospholipase D Genes Putatively Involved in Internal Browning of Pineapple during Postharvest Storage

An Advanced Lipid Metabolism System Revealed by Transcriptomic and Lipidomic Analyses Plays a Central Role in Peanut Cold Tolerance

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