Lipid Regulation Effects of Raw and Processed Notoginseng Radix Et Rhizome on Steatotic Hepatocyte L02 Cell

Chen, Zhu; Li, Chunmei; Yang, Chunjuan; Zhao, Ronghua; Mao, Xiaojian; Yu, Jie

doi:10.1155/2016/2919034

Cited by 12 publications

(12 citation statements)

References 10 publications

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“…Lau et al [9] compared the bleeding time (BT) in rats orally treated with raw and steamed PN roots and found that the treatment with raw PN extract resulted in shorter BT compared with rats treated with the steamed PN extract. Besides, raw PN extract displayed a much better lipid-lowering effect than steamed PN by investigating the levels of total cholesterol and triglyceride in steatotic L02 cells [10]. Such difference might be attributed to the change in the contents, relative proportion, and structures of chemical constituents in PN.…”

Section: Introductionmentioning

confidence: 97%

Chemical and bioactive comparison of Panax notoginseng root and rhizome in raw and steamed forms

Xiong

Chen

Man

et al. 2019

Journal of Ginseng Research

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Background The root and rhizome are historically and officially utilized medicinal parts of Panax notoginseng (PN) (Burk.) F. H. Chen, which in raw and steamed forms are used differently in practice. Methods To investigate the differences in chemical composition and bioactivities of PN root and rhizome between raw and steamed forms, high-performance liquid chromatography analyses and pharmacologic effects evaluated by tests of anticoagulation, antioxidation, hemostasis, antiinflammation, and hematopoiesis were combined. Results With the duration of steaming time, the contents of ginsenosides Rg 1 , Re, Rb 1 , Rd, and notoginsenoside R 1 in PN were decreased, while those of ginsenosides Rh 1 , 20( S )-Rg 3 , 20( R )-Rg 3 , Rh 4 , and Rk 3 were increased gradually. Raw PN samples steamed for 6 h at 120°C with stable levels of most constituents were used for the subsequent study of bioeffects. Raw PN showed better hemostasis, anticoagulation, and antiinflammation effects, while steamed PN exhibited stronger antioxidation and hematopoiesis activities. For different parts of PN, contents of saponins in PN rhizome were generally higher than those in the root, which could be related to the stronger bioactivities of rhizome compared with the same form of PN root. Conclusion This study provides basic information about the chemical and bioactive comparison of PN root and rhizome in both raw and steamed forms, indicating that the change of saponins may have a key role in different properties of raw and steamed PN.

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Section: Introductionmentioning

confidence: 97%

Chemical and bioactive comparison of Panax notoginseng root and rhizome in raw and steamed forms

Xiong

Chen

Man

et al. 2019

Journal of Ginseng Research

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“…LC-QQQ-MS/MS analysis revealed that isoquercitrin, typhaneoside, isorhamnetin-3-O-neohesperidoside, and notoginsenoside R1 were the chemical components with high contents in these extracts. In addition, previous in vivo and in vitro studies have found that alisol B 23-acetate, alisol A 24-acetate, nuciferine, rutin, isoquercitrin, quercetin, isorhamnetin, and notoginsenoside R1 were effective in protecting against hepatic steatosis [ 23 – 29 ]. Therefore, these compounds may be identified as potential chemical markers, which may provide a chemical basis for the study of Q-markers in HGQZ.…”

Section: Discussionmentioning

confidence: 99%

Discovery of Quality Markers in Hugan Qingzhi Formula by Integrating a Lipid‐Lowering Bioassay with UHPLC‐QQQ‐MS/MS

Xiao

Wang

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Nonalcoholic fatty liver disease (NAFLD) is a prevalent chronic liver disease. The Hugan Qingzhi formula (HGQZ) has been proven effective in treating NAFLD through clinical and pharmacological mechanism studies. A screening study of the chemical components was carried out to better control the quality of this formula. Current research has combined biological activity assessment with chemical analysis to screen and identify the bioactive compounds in HGQZ for use as potential quality markers (Q-markers) to control the quality of this herbal product. The HGQZ extracted by three different solvents was evaluated in a free fatty acid-induced hepatic steatosis LO2 cell model. Simultaneously, the twelve major chemical constituents of these extracts were quantitatively measured by ultrahigh-performance liquid chromatography coupled with triple quadrupole mass spectrometry (UHPLC-QQQ-MS/MS). Extraction with 50% ethanol showed the most potent lipid-lowering effect in steatosis LO2 cells and the highest extraction rate of major chemical constituents. Correlation analysis was used to establish the relationship between the biological activities and chemical characteristics of these extracts. The results showed that the contents of typhaneoside, hyperoside, isoquercitrin, isorhamnetin-3-O-neohesperidoside, notoginsenoside R1, and alisol B 23-acetate were positively correlated to the lipid-lowering effect. The subsequent bioassay confirmed that typhaneoside, isoquercitrin, and alisol B 23-acetate played the role of reducing the lipid effect. In conclusion, 50% of ethanol extraction produced the most active extract of HGQZ. Typhaneoside, isoquercitrin, and alisol B 23-acetate could be considered potential Q-markers for the quality control of HGQZ.

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“…GRb1 isolated from ginseng, another traditional Chinese medicine, has been proven to have therapeutic effects on treating obesity and diabetes [ 19 – 21 ]. Additionally, GRb1 is involved in IR via 11beta-hydroxysteroid dehydrogenase type I in our previous study [ 22 ] and NAFLD in other's study [ 23 ]. Moreover, GRb1 showed the ability to activate PPAR- γ [ 24 ] and be involved in the regulation of IR [ 25 ].…”

Section: Introductionmentioning

confidence: 92%

Ginsenoside Rb1 Alleviated High-Fat-Diet-Induced Hepatocytic Apoptosis via Peroxisome Proliferator-Activated Receptor γ

Song¹,

Sun

Chu

et al. 2020

BioMed Research International

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Objective. High-fat-diet- (HFD-) induced hepatic cell apoptosis is common in mice with nonalcoholic fatty liver disease (NAFLD). We aim to investigate the effect of Ginsenoside Rb1 (GRb1) on hepatocyte apoptosis. Methods. C57BL/6J mice with HFD were used to induce a liver-injured model with cell apoptosis. In addition, GRb1 was used to treat HFD-induced apoptosis in a liver with or without inhibitor of peroxisome proliferator-activated receptor γ (PPAR-γ). Results. Compared with C57BL/6J mice with common chow, there are downregulated PPAR-γ but upregulated cell apoptosis in the liver of mice with HFD. Furthermore, GRb1 elevated the hepatic PPAR-γ level and suppressed hepatocytic apoptosis. However, GW9662 abolished the effects of GRb1 on apoptosis in the liver. Conclusions. GRb1 alleviated HFD-induced apoptosis of hepatocytes of mice via PPAR-γ.

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Lipid Regulation Effects of Raw and Processed Notoginseng Radix Et Rhizome on Steatotic Hepatocyte L02 Cell

Cited by 12 publications

References 10 publications

Chemical and bioactive comparison of Panax notoginseng root and rhizome in raw and steamed forms

Chemical and bioactive comparison of Panax notoginseng root and rhizome in raw and steamed forms

Discovery of Quality Markers in Hugan Qingzhi Formula by Integrating a Lipid‐Lowering Bioassay with UHPLC‐QQQ‐MS/MS

Ginsenoside Rb1 Alleviated High-Fat-Diet-Induced Hepatocytic Apoptosis via Peroxisome Proliferator-Activated Receptor γ

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