Lipid rafts of purified mouse brain synaptosomes prepared with or without detergent reveal different lipid and protein domains

Eckert, Gunter P.; Igbavboa, Urule; Müller, Wernér E.G.; Wood, W. Gibson

doi:10.1016/s0006-8993(02)03986-0

Cited by 76 publications

(88 citation statements)

References 32 publications

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“…When cells are disrupted in the presence of hypertonic sodium carbonate (82,83) or by the mean of mechanical treatments (sonication under carefully controlled conditions) (84), and lysates are subjected to density gradient centrifugation, low-density membrane fractions are obtained that are very similar in composition, even if not identical (48,56,58,62,72,73,83,(85)(86)(87)(88)(89)(90)(91)(92), suggesting that the low-density membrane fraction composition correspond to that of physiological lipid membrane domains, and that it is not determined by a random rearrangement of cell components induced by the experimental conditions used in their preparation (48,53,63,64,66,72,(84)(85)(86)91).…”

Section: Preparation Of Detergent-resistant Membrane (Drm) Fractionsmentioning

confidence: 99%

Membrane lipid domains and membrane lipid domain preparations: are they the same thing?

Sonnino

Prinetti

2008

TIGG

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Section: Preparation Of Detergent-resistant Membrane (Drm) Fractionsmentioning

confidence: 99%

Membrane lipid domains and membrane lipid domain preparations: are they the same thing?

Sonnino

Prinetti

2008

TIGG

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“…Another negative control was carried out to confirm the specificity of the association of these proteins with lipid rafts. Fluorescence of crosslinked CT-B bound to GM1 was compared to that for immunofluorescence staining of Na+/K+-ATPase, which does not associate with lipid rafts and is recovered in the heavy fractions in the separation of detergent-resistant membranes [14,33,34]. As shown in Fig.…”

Section: Lipid Raft Content Of Membranes Is Altered In Slo-hkmentioning

confidence: 99%

7-Dehydrocholesterol enhances ultraviolet A-induced oxidative stress in keratinocytes: Roles of NADPH oxidase, mitochondria, and lipid rafts

Valencia

Rajadurai

Carle

et al. 2006

Free Radical Biology and Medicine

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Long wavelength solar UVA radiation stimulates formation of reactive oxygen species (ROS) and prostaglandin E 2 (PGE 2 ), which are involved in skin photosensitivity and tumor promotion. High levels of 7-dehydrocholesterol (7-DHC), the precursor to cholesterol, cause exaggerated photosensitivity to UVA in patients with Smith-Lemli-Opitz syndrome (SLOS). Partially replacing cholesterol with 7-DHC in keratinocytes rapidly (<5 min) increased UVA-induced ROS, intracellular calcium, phospholipase A 2 activity, PGE 2 , and NADPH oxidase activity. UVA-induced ROS and PGE 2 production were inhibited in these cells by depleting the Nox1 subunit of NADPH oxidase using siRNA or using a mitochondrial radical quencher, MitoQ. Partial replacement of cholesterol with 7-DHC also disrupted membrane lipid raft domains, although depletion of cholesterol, which also disrupts lipid rafts, did not affect UVA-induced increases in ROS and PGE 2 . Phospholipid liposomes containing 7-DHC were more rapidly oxidized by a free radical mechanism than those containing cholesterol. These results indicate that 7-DHC enhances rapid UVA-induced ROS and PGE 2 formation by enhancing free radical-mediated membrane lipid oxidation and suggests that this mechanism might underlie the UVA-photosensitivity in SLOS.

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“…Since it was suspected that the presence of detergent could produce artifacts, the use of non-detergent methods has been suggested [8,20]. Accordingly, studies on brain synaptosomes revealed differences both qualitatively and quantitatively in proteins and lipids when detergent and nondetergent methods were applied [20,21]. Beside the biochemical characterization of isolated membrane fractions, different techniques for studying lipid rafts in situ such as functional imaging, cytometry, two-photon microscopy, electron microscopy, fluorescent-quenching and resonance energy transfer techniques have been developed [22][23][24][25][26][27][28][29].…”

Section: Impact Of Lipid Rafts For Physiological and Pathophysiologicmentioning

confidence: 99%

Manipulation of Lipid Rafts in Neuronal Cells~!2009-09-18~!2009-12-16~!2010-03-19~!

Eckert¹

2010

TOBIOJ

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Lipid rafts are specialized plasma membrane micro-domains highly enriched in cholesterol, sphingolipids and glycosylphosphatidylinositol (GPI) anchored proteins. Lipid rafts are thought to be located in the exofacial leaflet of plasma membranes. Functionally, lipid rafts are involved in intracellular trafficking of proteins and lipids, secretory and endocytotic pathways, signal transduction, inflammation and in cell-surface proteolysis. There has been substantial interest in lipid rafts in brain, both with respect to normal functioning and with certain neurodegenerative diseases. Based on the impact of lipid rafts on multitude biochemical pathways, modulation of lipid rafts is used to study related disease pathways and probably offers a target for pharmacological intervention. Lipid rafts can be targeted by modulation of its main components, namely cholesterol and sphingolipids. Other approaches include the modulation of membrane dynamics and it has been reported that protein-lipid interactions can vary the occurrence and composition of these membrane micro-domains. The present review summarizes the possibilities to modulate lipid rafts with focus on neuronal cells.

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Lipid rafts of purified mouse brain synaptosomes prepared with or without detergent reveal different lipid and protein domains

Cited by 76 publications

References 32 publications

Membrane lipid domains and membrane lipid domain preparations: are they the same thing?

Membrane lipid domains and membrane lipid domain preparations: are they the same thing?

7-Dehydrocholesterol enhances ultraviolet A-induced oxidative stress in keratinocytes: Roles of NADPH oxidase, mitochondria, and lipid rafts

Manipulation of Lipid Rafts in Neuronal Cells~!2009-09-18~!2009-12-16~!2010-03-19~!

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