Lipid profiling and corresponding biodiesel quality of Mortierella isabellina using different drying and extraction methods

Hussain, Javid; Ruan, Zhenhua; Nascimento, Iracema Andrade; Liu, Yan; Liao, Wei

doi:10.1016/j.biortech.2014.06.074

Cited by 27 publications

(16 citation statements)

References 18 publications

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“…Total lipid was extracted (Bligh & Dyer, 1959) and its fatty acid profile determination was performed using an Agilent 6890N gas chromatograph equipped with an Agilent DB-23 column (30 m 250 um x 0.25 um) and a CTC PAL auto sampler. The gas chromatography (GC) was connected to an Agilent 5975 single quadruple mass spectrometer equipped with a 70 eV electron ionization source (Hussain et al, 2014). Protein content in the supernatant was determined using a BCA kit from Sigma.…”

Section: Methodsmentioning

confidence: 99%

Effects of inhibitory compounds in lignocellulosic hydrolysates on Mortierella isabellina growth and carbon utilization

Ruan

Hollinshead

Isaguirre

et al. 2015

Bioresource Technology

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Section: Methodsmentioning

confidence: 99%

Effects of inhibitory compounds in lignocellulosic hydrolysates on Mortierella isabellina growth and carbon utilization

Ruan

Hollinshead

Isaguirre

et al. 2015

Bioresource Technology

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“…Biodiesel quality can be predicted from the FA profile of the feedstock [13]. Therefore, various researchers have developed, and used, numerical models to estimate biodiesel quality indicators including density, cetane number, iodine value, and cold filter plugging point (CFPP), using FA profiles [14][15][16][17][18][19]. To assess the quality of biodiesel produced from Azolla using these numerical models, quantitative information on its lipid composition is required.…”

Section: Introductionmentioning

confidence: 99%

Lipid Yield and Composition of Azolla filiculoides and the Implications for Biodiesel Production

Brouwer

Werf²,

Schluepmann

et al. 2015

Bioenerg. Res.

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The aquatic fern Azolla is one of the fastestgrowing nitrogen-fixing plants on Earth and therefore considered as a potential source of biomass for bioenergy production. The lipid fraction from Azolla filiculoides was analyzed to investigate whether it suited biodiesel production. Since the productivity of Azolla is further increased at higher CO 2 concentrations, A. filiculoides biomass was produced at 800 ppm CO 2 mimicking a cultivation system utilizing CO 2 waste from industry. The harvested biomass contained 7.92±0.14 % dry weight (dw) crude lipids. Drying conditions did not significantly affect lipid composition or yields, indicating that drying conditions may be energetically optimized without the risk of product loss. Total lipid extracts contained 4.2±0.38 % free fatty acids. Of the crude lipid fraction, 41±13 % consisted of fatty acids that were converted into fatty acid methyl esters upon saponification in methanol. Unique mid-chain (di)hydroxy compounds constituted 7.2±2.8 % of the crude lipids. Based on the fatty acid profile, it was estimated that Azolla biodiesel meets requirements set by the EN14214 standard on fuel density, cetane number, and iodine value. The cold filter plugging point (CFPP), however, is expected to be too high due to relatively high concentrations of lignoceric acid and the presence of the mid-chain (di)hydroxy compounds. To produce high-quality biodiesel from Azolla lipids, therefore, a fractionation step will be required removing these compounds. As an advantage, the long-chain alcohols and (di)hydroxy fatty acids obtained after fractionation may provide a valuable secondary product stream with applications to chemical industry and nutrition.

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“…[30][31][32]34,35 It has been reported that M. isabellina can effectively utilize not only glucose and xylose but also acetic acid as its carbon sources for lipid accumulation. 38 Therefore, M. isabellina fermentation was used by this study to carry out the system analysis. 36 Moreover, the lipid profiles of M. isabellina are shown to be of good quality for biodiesel production.…”

Section: Introductionmentioning

confidence: 99%

A sustainable lignocellulosic biodiesel production integrating solar- and bio-power generation

et al. 2016

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A solar-bio-powered refining concept has been developed to establish sustainable lignocellulosic biodiesel production. The refining concept applies a combined hydrolysis process without detoxification to release sugars from corn stover, uses aerobic oleaginous fungal fermentation to accumulate lipids on the hydrolysate for biodiesel production, and implements solar-bio-power generation to satisfy the energy demand. The results demonstrate that system integration of solar-bio-power generation and fungal lipid accumulation is an effective approach to create a sustainable lignocellulosic biodiesel production. Compared to concentrated solar power (CSP) units, photovoltaics (PV) are a more suitable solar technology to satisfy the energy needs of the lignocellulosic biodiesel production. The PV-bio-powered lignocellulosic biodiesel production has a net energy output of 28.70 MJ kg −1 biodiesel produced, and a net energy ratio (NER) of 1.08. † Electronic supplementary information (ESI) available. See

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Lipid profiling and corresponding biodiesel quality of Mortierella isabellina using different drying and extraction methods

Cited by 27 publications

References 18 publications

Effects of inhibitory compounds in lignocellulosic hydrolysates on Mortierella isabellina growth and carbon utilization

Effects of inhibitory compounds in lignocellulosic hydrolysates on Mortierella isabellina growth and carbon utilization

Lipid Yield and Composition of Azolla filiculoides and the Implications for Biodiesel Production

A sustainable lignocellulosic biodiesel production integrating solar- and bio-power generation

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