Lipid production from biodiesel-derived crude glycerol by Rhodosporidium fluviale DMKU-RK253 using temperature shift with high cell density

Polburee, Pirapan; Yongmanitchai, Wichien; Honda, Kohsuke; Ohashi, Takao; Yoshida, Takemi; Fujiyama, Kazuhito; Limtong, Savitree

doi:10.1016/j.bej.2016.04.024

Cited by 45 publications

(11 citation statements)

References 41 publications

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“…250 g/L) . Moreover, the conversion of glycerol into SCO has been seen in R. toruloides or other red strains principally the last years . The reported L max value ( c .…”

Section: Discussionmentioning

confidence: 96%

Production of secondary metabolites through glycerol fermentation under carbon‐excess conditions by the yeasts Yarrowia lipolytica and Rhodosporidium toruloides

Papanikolaou

Kampisopoulou

Blanchard

et al. 2017

Euro J Lipid Sci & Tech

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The biochemical behavior of the yeasts Rhodosporidium toruloides DSM 4444, Yarrowia lipolytica ACA YC 5029, and Y. lipolytica ACA YC 5030 was evaluated on waste glycerol‐based media, added at increasing initial concentrations (50–140 g/L), with nitrogen remaining constant. During flask cultivation of Y. lipolytica ACA‐YC 5030, and although medium pH remained >4.8, major metabolites were the polyols mannitol (CMl) and erythritol (CEr) (CMlmax = 32.1 g/L, conversion yield per glycerol consumed =0.23 g/g, and CErmax = 35.5 g/L, yield ≈0.25 g/g). For the strain ACA‐YC 5029, equally in flasks and pH >4.8, the major metabolites were again mannitol and erythritol, with CMlmax = 28.9 g/L (conversion yield ≈0.21 g/g), and CErmax = 33.6 g/L (yield ≈0.24 g/g). In contrast, in batch‐bioreactor experiment, the yeast ACA YC‐5029 produced mainly citric acid (maximum concentration =39 g/L, yield on glycerol =0.42 g/g). R. toruloides DSM 4444 was flask‐cultured at increasing initial glycerol quantities (up to ≈140 g/L) and displayed significant growth producing also satisfactory amounts of lipids. The maximum DCW concentration achieved was 39.1 g/L. Maximum lipids were 13.7 g/L (at that point lipid in DCW was 37.0% w/w). The major cellular fatty acid produced was oleic acid. Phospholipids of R. toruloides were the most unsaturated fraction among lipid fractions quantified. Practical applications: Industrial glycerol, the main by‐product of biodiesel‐producing facilities, appears in constantly increasing quantities in the market volume due to the worldwide rise of biodiesel production, rendering the valorization of this by‐product as a very important scientific priority. In this report, the yeasts Y. lipolytica ACA YC 5029 and Y. lipolytica ACA YC 5030 were cultivated in submerged batch experiments with industrial glycerol as substrate under nitrogen limitation and appreciable quantities of mannitol and erythritol, compounds presenting significant importance for the food industries, were synthesized. The yeast R. toruloides DSM 4444, equally batch‐cultured on glycerol under nitrogen limitation, produced appreciable quantities of biomass that contained high concentrations of microbial lipids, that could be transformed into “2nd generation” biodiesel. The current study, hence, provides viable possibilities of glycerol valorization through its use as substrate in order metabolic compounds of added‐value to be synthesized. Crude glycerol, the principal “waste” stream deriving from biodiesel production process, is converted with the aid of microbial fermentations into secondary metabolites useful for the food‐ and biofuels‐producing industries. The metabolic compounds synthesized are microbial lipids (single cell oils) amenable to be converted into 2nd generation biodiesel with fermentations led by a strain of the yeast Rhodosporidium toruloides and mannitol, erythritol and citric acid with fermentations led by strains of the yeast Yarrowia lipolytica.

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“…250 g/L) . Moreover, the conversion of glycerol into SCO has been seen in R. toruloides or other red strains principally the last years . The reported L max value ( c .…”

Section: Discussionmentioning

confidence: 96%

Production of secondary metabolites through glycerol fermentation under carbon‐excess conditions by the yeasts Yarrowia lipolytica and Rhodosporidium toruloides

Papanikolaou

Kampisopoulou

Blanchard

et al. 2017

Euro J Lipid Sci & Tech

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“…In the first stage, the cells are cultivated in a nutrient-rich medium to promote cell propagation. Then, the cells are transferred to a carbon source solution without auxiliary nutrients to enhance lipid accumulation in the second stage [21]. The deficiency of nutrients, particularly nitrogen or phosphorous, discontinued the biosynthesis of protein and nucleic acids and so inhibited, cell proliferation.…”

Section: Introductionmentioning

confidence: 99%

“…Nevertheless, the carbon source was more efficiently converted into lipids, leading to higher lipid productivity and yield [22]. The procedure of two-stage cultivation usually consists of the cultivation of cells in a nutrient-rich medium to obtain high cell density; then, cells are collected from the culture broth by centrifugation and resuspended in a carbon source solution, such as xylose solution [23], pure glycerol [22], and crude glycerol solution [21,22], without auxiliary nutrients. While such procedures are practical in shake-flask cultivation; they are difficult to perform in large-scale production as in a fermenter.…”

Section: Introductionmentioning

confidence: 99%

Feeding Strategies of Two-Stage Fed-Batch Cultivation Processes for Microbial Lipid Production from Sugarcane Top Hydrolysate and Crude Glycerol by the Oleaginous Red Yeast Rhodosporidiobolus fluvialis

Poontawee

2020

Microorganisms

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Microbial lipids are able to produce from various raw materials including lignocellulosic biomass by the effective oleaginous microorganisms using different cultivation processes. This study aimed to enhance microbial lipid production from the low-cost substrates namely sugarcane top hydrolysate and crude glycerol by Rhodosporidiobolus fluvialis DMKU-SP314, using two-stage fed-batch cultivation with different feeding strategies in a 3 L stirred-tank fermenter. The effect of two feeding strategies of 147.5 g/L crude glycerol solution was evaluated including pulse feeding at different starting time points (48, 24, and 72 h after initiation of batch operation) and constant feeding at different dilution rates (0.012, 0.020, and 0.033 h−1). The maximum lipid concentration of 23.6 g/L and cell mass of 38.5 g/L were achieved when constant feeding was performed at the dilution rate of 0.012 h−1 after 48 h of batch operation, which represented 1.24-fold and 1.27-fold improvements in the lipid and cell mass concentration, respectively. Whereas, batch cultivation provided 19.1 g/L of lipids and 30.3 g/L of cell mass. The overall lipid productivity increased to 98.4 mg/L/d in the two-stage fed-batch cultivation. This demonstrated that the two-stage fed-batch cultivation with constant feeding strategy has the possibility to apply for large-scale production of lipids by yeast.

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“…This demonstrated that consideration of only the initial high C/N ratio is not always sufficient for high lipid production. Various cultivation processes have been reported for lipid production in yeast; i.e., batch [16][17][18], fed-batch [19][20][21] and two-stage cultivations [22,23]. In two-stage cultivation strategies, cell division and lipid accumulation modes are spatially and/or temporally separated to achieve a high cell mass and high lipid productivity.…”

Section: Introductionmentioning

confidence: 99%

“…Crude glycerol (CG), a byproduct from biodiesel production plants which has been shown to have some inhibitory compounds to microorganism growth, is currently being explored as a possible large-scale carbon source in lipid production by many researchers. Many studies have demonstrated that various oleaginous yeasts have a great capacity to convert CG into lipids with high yields [23,[31][32][33][34].…”

Section: Introductionmentioning

confidence: 99%

Lipid Production from Sugarcane Top Hydrolysate and Crude Glycerol with Rhodosporidiobolus fluvialis Using a Two-Stage Batch-Cultivation Strategy with Separate Optimization of Each Stage

et al. 2020

Self Cite

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Lipids from oleaginous microorganisms, including oleaginous yeasts, are recognized as feedstock for biodiesel production. A production process development of these organisms is necessary to bring lipid feedstock production up to the industrial scale. This study aimed to enhance lipid production of low-cost substrates, namely sugarcane top and biodiesel-derived crude glycerol, by using a two-stage cultivation process with Rhodosporidiobolus fluvialis DMKU-SP314. In the first stage, sugarcane top hydrolysate was used for cell propagation, and in the second stage, cells were suspended in a crude glycerol solution for lipid production. Optimization for high cell mass production in the first stage, and for high lipid production in the second stage, were performed separately using a one-factor-at-a-time methodology together with response surface methodology. Under optimum conditions in the first stage (sugarcane top hydrolysate broth containing; 43.18 g/L total reducing sugars, 2.58 g/L soy bean powder, 0.94 g/L (NH4)2SO4, 0.39 g/L KH2PO4 and 2.5 g/L MgSO4 7H2O, pH 6, 200 rpm, 28 °C and 48 h) and second stage (81.54 g/L crude glycerol, pH 5, 180 rpm, 27 °C and 196 h), a high lipid concentration of 15.85 g/L, a high cell mass of 21.07 g/L and a high lipid content of 73.04% dry cell mass were obtained.

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Lipid production from biodiesel-derived crude glycerol by Rhodosporidium fluviale DMKU-RK253 using temperature shift with high cell density

Cited by 45 publications

References 41 publications

Production of secondary metabolites through glycerol fermentation under carbon‐excess conditions by the yeasts Yarrowia lipolytica and Rhodosporidium toruloides

Production of secondary metabolites through glycerol fermentation under carbon‐excess conditions by the yeasts Yarrowia lipolytica and Rhodosporidium toruloides

Feeding Strategies of Two-Stage Fed-Batch Cultivation Processes for Microbial Lipid Production from Sugarcane Top Hydrolysate and Crude Glycerol by the Oleaginous Red Yeast Rhodosporidiobolus fluvialis

Lipid Production from Sugarcane Top Hydrolysate and Crude Glycerol with Rhodosporidiobolus fluvialis Using a Two-Stage Batch-Cultivation Strategy with Separate Optimization of Each Stage

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