Lipid production by microalga Micractinium sp. IC-76 in a flat panel photobioreactor and its transesterification with cross-linked enzyme aggregates of Burkholderia cepacia lipase

Piligaev, A. V.; Sorokina, K. N.; Samoylova, Yu. V.; Parmon, Valentin N.

doi:10.1016/j.enconman.2017.10.086

Cited by 32 publications

(7 citation statements)

References 54 publications

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“…The results showed that the conversion was above 80 mol% during the first five cycles, decreasing by 10% with respect to the first reaction. Subsequently, the conversion gradually decreased, reaching a conversion of around 70 mol% after the tenth reuse These results are comparable although better than others reported in the literature for the lipase from P. cepacia where after the first use, 10% of the activity was lost and in the fourth cycle the activity was reduced by 53% [41]. This loss of activity can be caused by a possible deterioration of the biocatalyst by enzyme poisoning [42].…”

Section: Study Of the Reuse Of The Heterogeneous Enzymatic Catalystsupporting

confidence: 80%

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Biodiesel Production (FAEEs) by Heterogeneous Combi-Lipase Biocatalysts Using Wet Extracted Lipids from Microalgae

et al. 2019

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The production of fatty acids ethyl esters (FAEEs) to be used as biodiesel from oleaginous microalgae shows great opportunities as an attractive source for the production of renewable fuels without competing with human food. To ensure the economic viability and environmental sustainability of the microbial biomass as a raw material, the integration of its production and transformation into the biorefinery concept is required. In the present work, lipids from wet Isochrysis galbana microalga were extracted with ethyl acetate with and without drying the microalgal biomass (dry and wet extraction method, respectively). Then, FAEEs were produced by lipase-catalyzed transesterification and esterification of the extracted lipids with ethanol using lipase B from Candida antarctica (CALB) and Pseudomonas cepacia (PC) lipase supported on SBA-15 mesoporous silica functionalized with amino groups. The conversion to FAEEs with CALB (97 and 85.5 mol% for dry and wet extraction, respectively) and PC (91 and 87 mol%) biocatalysts reached higher values than those obtained with commercial Novozym 435 (75 and 69.5 mol%). Due to the heterogeneous nature of the composition of microalgae lipids, mixtures with different CALB:PC biocatalyst ratio were used to improve conversion of wet-extracted lipids. The results showed that a 25:75 combi-lipase produced a significantly higher conversion to FAEEs (97.2 mol%) than those produced by each biocatalyst independently from wet-extracted lipids and similar ones than those obtained by each lipase from the dry extraction method. Therefore, that optimized combi-lipase biocatalyst, along with achieving the highest conversion to FAEEs, would allow improving viability of a biorefinery since biodiesel production could be performed without the energy-intensive step of biomass drying.

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Section: Study Of the Reuse Of The Heterogeneous Enzymatic Catalystsupporting

confidence: 80%

“…These nuclei resonate at 79. 41 MHz. An H/X 7.5 mm MAS probe and ZrO2 rotors spinning at 6 kHz were used.…”

Section: Characterization Of Biocatalystsmentioning

confidence: 99%

Biodiesel Production (FAEEs) by Heterogeneous Combi-Lipase Biocatalysts Using Wet Extracted Lipids from Microalgae

et al. 2019

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“…In line with this, the maximal lipid production in MTA PBR (118 mg L −1 d −1 ) was only 11% lower than the maximum values reported up to date [48]. Also, lipid yields of different microalgae strains cultivated in (pilot scale) flat panel PBRs (36-91 mg L −1 d −1 ) correspond well with our results [49][50][51], same as the lipid content (41-47%) and calorific values (24-25 MJ kg −1 , [30,39,52]). Considering the suboptimal cultivation conditions in our setup, the comparative lipid yields suggest possible lipid production limitation in the previous studies.…”

Section: Biomass and Lipid Productivitysupporting

confidence: 91%

Perspective Design of Algae Photobioreactor for Greenhouses—A Comparative Study

et al. 2021

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The continued growth and evolving lifestyles of the human population require the urgent development of sustainable production in all its aspects. Microalgae have the potential of the sustainable production of various commodities; however, the energetic requirements of algae cultivation still largely contribute to the overall negative balance of many operation plants. Here, we evaluate energetic efficiency of biomass and lipids production by Chlorella pyrenoidosa in multi-tubular, helical-tubular, and flat-panel airlift pilot scale photobioreactors, placed in an indoor environment of greenhouse laboratory in Central Europe. Our results show that the main energy consumption was related to the maintenance of constant light intensity in the flat-panel photobioreactor and the culture circulation in the helical-tubular photobioreactor. The specific power input ranged between 0.79 W L−1 in the multi-tubular photobioreactor and 6.8 W L−1 in the flat-panel photobioreactor. The construction of multi-tubular photobioreactor allowed for the lowest energy requirements but also predetermined the highest temperature sensitivity and led to a significant reduction of Chlorella productivity in extraordinary warm summers 2018 and 2019. To meet the requirements of sustainable yearlong microalgal production in the context of global change, further development towards hybrid microalgal cultivation systems, combining the advantages of open and closed systems, can be expected.

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“…CLEAs biocatalysts have high activity and stability over a wide range of pH (4.0-11.0) [23], temperature (10-90 • C) [24,25], and a high tolerance to chemical reagents including phenylmethylsulfonyl fluoride (PMSF), sodium dodecyl sulfate (SDS), ethanol, and phenol [26]. Such biocatalysts were previously successfully applied for the production of biodiesel [27,28] and chemicals [29], but not for insecticide degradation.…”

Section: Introductionmentioning

confidence: 99%

Preparation of Stable Cross-Linked Enzyme Aggregates (CLEAs) of a Ureibacillus thermosphaericus Esterase for Application in Malathion Removal from Wastewater

et al. 2018

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In this study, the active and stable cross-linked enzyme aggregates (CLEAs) of the thermostable esterase estUT1 of the bacterium Ureibacillus thermosphaericus were prepared for application in malathion removal from municipal wastewater. Co-expression of esterase with an E. coli chaperone team (KJE, ClpB, and ELS) increased the activity of the soluble enzyme fraction up to 200.7 ± 15.5 U mg−1. Response surface methodology (RSM) was used to optimize the preparation of the CLEA-estUT1 biocatalyst to maximize its activity and minimize enzyme loss. CLEA-estUT1 with the highest activity of 29.4 ± 0.5 U mg−1 (90.6 ± 2.7% of the recovered activity) was prepared with 65.1% (w/v) ammonium sulfate, 120.6 mM glutaraldehyde, and 0.2 mM bovine serum albumin at 5.1 h of cross-linking. The biocatalyst has maximal activity at 80 °С and pH 8.0. Analysis of the properties of CLEA-estUT1 and free enzyme at 50–80 °C and pH 5.0–10.0 showed higher stability of the biocatalyst. CLEA-estUT1 showed marked tolerance against a number of chemicals and high operational stability and activity in the reaction of malathion hydrolysis in wastewater (up to 99.5 ± 1.4%). After 25 cycles of malathion hydrolysis at 37 °С, it retained 55.2 ± 1.1% of the initial activity. The high stability and reusability of CLEA-estUT1 make it applicable for the degradation of insecticides.

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Lipid production by microalga Micractinium sp. IC-76 in a flat panel photobioreactor and its transesterification with cross-linked enzyme aggregates of Burkholderia cepacia lipase

Cited by 32 publications

References 54 publications

Biodiesel Production (FAEEs) by Heterogeneous Combi-Lipase Biocatalysts Using Wet Extracted Lipids from Microalgae

Biodiesel Production (FAEEs) by Heterogeneous Combi-Lipase Biocatalysts Using Wet Extracted Lipids from Microalgae

Perspective Design of Algae Photobioreactor for Greenhouses—A Comparative Study

Preparation of Stable Cross-Linked Enzyme Aggregates (CLEAs) of a Ureibacillus thermosphaericus Esterase for Application in Malathion Removal from Wastewater

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