Lipid production by Lipomyces starkeyi using sap squeezed from felled old oil palm trunks

Juanssilfero, Ario Betha; Kahar, Prihardi; Amza, Rezky Lastinov; Yopi, -; Sudesh, Kumar; Prasetya, Bambang; Kondo, Akihiko

doi:10.1016/j.jbiosc.2018.12.002

Cited by 13 publications

(4 citation statements)

References 33 publications

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“…The composition of the fatty acids in the immobilized culture included concentrations of oleic acid (21.6%) and linoleic acid (25.1%), which were slightly higher than those found in the free-cell culture (20.4% and 24.9%, respectively). The fatty acid profile of palm oil, which is one of the most utilized feedstocks for biodiesel production, contains mainly oleic and palmitic acids [32], which is similar to the fatty acid profile obtained by immobilized C. sorokiniana, indicating that these lipids have the potential to be used as biodiesel feedstock.…”

Section: Lipid Productivity Of Immobilized C Sorokinianasupporting

confidence: 58%

Enhanced growth and lipid productivity by living Chlorella sorokiniana immobilized in Ca-alginate beads

et al. 2023

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The bottleneck for the production of biofuels from microalgae consists on costly harvesting processes and low lipid production, immobilization technology could play a part on making the production of biofuels more feasible. The aim of this study was to evaluate the effect of alginate immobilization on the growth and lipid productivity of the microalgae Chlorella sorokiniana, so far, the main focus of immobilization technology has been its use for wastewater treatment and nutrient removal from effluents. The microalgae Chlorella sorokiniana was cultured in both free and immobilized forms under optimal autotrophic growth conditions. Microalgae were immobilized in calcium alginate beads generated by mixing algal cells with a sodium alginate solution, followed by extrusion into a CaCl2 solution. The results obtained in this study showed that the growth of the microalgae immobilized in alginate beads, was enhanced and achieved a dry cell weight 1.4-fold higher than that of a free cell culture, a higher light transmittance was also achieved in the alginate immobilized culture, and the lipid productivity was increased from 54.21 ± 2.48 mg/L/d in the free cell culture to 82.22 ± 8.48 mg/L/d in the immobilized culture. These results demonstrate the effectiveness of immobilization technology for promoting growth and lipid productivity in the microalgae Chlorella sorokiniana.

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Section: Lipid Productivity Of Immobilized C Sorokinianasupporting

confidence: 58%

Enhanced growth and lipid productivity by living Chlorella sorokiniana immobilized in Ca-alginate beads

et al. 2023

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“…lactose); high inhibitor tolerance; fast growth – [ 28 , 30 , 31 , 118 , 121 ] Lipomyces starkeyi 7.8 (7.5 + 0.3) 1.60 21 (est.) 33.3 2.8 4.8 52.0 3.6 Co-fermentation of substrates – [ 109 , 148 , 198 , 266 , 316 ] Rhodotorula glutinis 5.4 (5.1 + 0.3) 0.86 210 (est.) 15.4 7.2 9.1 63.5 1.8 Bio-control ability; valuable co-product: carotenoids – [ 97 , 124 , 204 , 205 , 290 ] Rhodotorula mucilaginosa 4.1 (3.7 + 0.4) 0.33 12 19.6 0.8 6.1 41.8 27.0 Co-products: xylitol, carotenoids – [ 304 , 312 , 317 ] Trichosporon cutaneum 2.8 (2.6 + 0.2) 0.17 14 30.0 – ...…”

Section: Oleaginous Yeast Speciesmentioning

confidence: 99%

“…Certain wild-type oleaginous yeasts such as Lipomyces lipofer [ 134 ], L. starkeyi [ 109 , 134 , 148 , 196 , 266 ], Macalpinomyces spermophorus [ 223 ], M. pulcherrima [ 54 ] and R. glutinis [ 120 , 267 ] have been shown to produce oil similar in composition to palm oil. This oil has the potential to be used for further processing or as a cooking oil in an unrefined state.…”

Section: Yeast Lipid Applicationsmentioning

confidence: 99%

The history, state of the art and future prospects for oleaginous yeast research

Abeln

Chuck

2021

Microb Cell Fact

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Lipid-based biofuels, such as biodiesel and hydroprocessed esters, are a central part of the global initiative to reduce the environmental impact of the transport sector. The vast majority of production is currently from first-generation feedstocks, such as rapeseed oil, and waste cooking oils. However, the increased exploitation of soybean oil and palm oil has led to vast deforestation, smog emissions and heavily impacted on biodiversity in tropical regions. One promising alternative, potentially capable of meeting future demand sustainably, are oleaginous yeasts. Despite being known about for 143 years, there has been an increasing effort in the last decade to develop a viable industrial system, with currently around 100 research papers published annually. In the academic literature, approximately 160 native yeasts have been reported to produce over 20% of their dry weight in a glyceride-rich oil. The most intensively studied oleaginous yeast have been Cutaneotrichosporon oleaginosus (20% of publications), Rhodotorula toruloides (19%) and Yarrowia lipolytica (19%). Oleaginous yeasts have been primarily grown on single saccharides (60%), hydrolysates (26%) or glycerol (19%), and mainly on the mL scale (66%). Process development and genetic modification (7%) have been applied to alter yeast performance and the lipids, towards the production of biofuels (77%), food/supplements (24%), oleochemicals (19%) or animal feed (3%). Despite over a century of research and the recent application of advanced genetic engineering techniques, the industrial production of an economically viable commodity oil substitute remains elusive. This is mainly due to the estimated high production cost, however, over the course of the twenty-first century where climate change will drastically change global food supply networks and direct governmental action will likely be levied at more destructive crops, yeast lipids offer a flexible platform for localised, sustainable lipid production. Based on data from the large majority of oleaginous yeast academic publications, this review is a guide through the history of oleaginous yeast research, an assessment of the best growth and lipid production achieved to date, the various strategies employed towards industrial production and importantly, a critical discussion about what needs to be built on this huge body of work to make producing a yeast-derived, more sustainable, glyceride oil a commercial reality.

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“…The pH‐value is also an important parameter for further fermentation processes. In different studies the following pH‐values were determined for the pressed sap: 4.5, 25 5, 53 5.16, 24 7.49 67 . Komonkiat and Cheirsilp 25 assumed that the pH‐value is more acidic when microbial growth was initiated, while fresh sap is supposed to possess a neutral pH‐value.…”

Section: Ingredients and Characteristics Of The Pressed Sap From Oil Palm Trunksmentioning

confidence: 99%

Pressed sap from oil palm (Elaeis guineensis) trunks: a revolutionary growth medium for the biotechnological industry?

Dirkes

Neubauer

Rabenhorst

2021

Biofuels Bioprod Bioref

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In recent decades, the demand for palm oil has constantly increased and with it the cultivation of oil palms. After a period of 25 years, the oil yield of the palm trees decreases and they are felled. The trees are cut into pieces and remain on the plantations. However, due to their high moisture and sugar content, fungi and molds cause problems for replanting. The use of the wood for the timber industry is difficult due to its structural characteristics. Biotechnological processes use microorganisms to produce relevant industrial products. The basis for each process is a culture medium that contains all necessary nutrients, especially carbohydrates. The culture medium makes up a high percentage of the costs, so alternative, cheaper substrates are preferred. In this review, we show and compare different analyses of the sap mechanically pressed from the oil palm trunk regarding its sugar and nutrient content. The total sugar concentration in the palm sap varies between 16.97–140 g L−1 and it is mainly composed of glucose, fructose, and sucrose. The comparison with common nutrient media and the results of fermentation processes already carried out on a laboratory scale show that palm sap offers great potential as a fermentation medium for biotechnological conversion into industrially relevant products. © 2021 The Authors Biofuels, Bioproducts and Biorefining published by Society of Chemical Industry and John Wiley & Sons, Ltd

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Lipid production by Lipomyces starkeyi using sap squeezed from felled old oil palm trunks

Cited by 13 publications

References 33 publications

Enhanced growth and lipid productivity by living Chlorella sorokiniana immobilized in Ca-alginate beads

Enhanced growth and lipid productivity by living Chlorella sorokiniana immobilized in Ca-alginate beads

The history, state of the art and future prospects for oleaginous yeast research

Pressed sap from oil palm (Elaeis guineensis) trunks: a revolutionary growth medium for the biotechnological industry?

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