Lipid production by Aspergillus oryzae from starch substrates

Kaur, Pinderpal; Worgan, J.T.

doi:10.1007/bf00500740

Cited by 10 publications

(7 citation statements)

References 11 publications

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“…41,42 On the other hand, A. oryzae biomass cultivated in PDB medium had 30.24% protein, 28.47% carbohydrate, and 25.12% lipid (Table 4). The value in A. oryzae biomassderived lipid content was lower than that stated by Kaur and Worgan but was higher than the one reported by Vorapreeda et al 43,44 With respect to the fatty acid composition, A. oryzae lipid had 25.79% PA, 11.18% stearic acid (C18:0, SA), 15.48% oleic acid (C18:1, OA), and 27.14% LA as the main fatty acids, as shown in Table 4. C16 and C18 fatty acids of A. oryzae lipid represented around 95%, manifesting that A. oryzae could synthesize valuable lipids with superior quality for biodiesel production.…”

Section: T H Icontrasting

confidence: 75%

“…The value in A. oryzae biomass-derived lipid content was lower than that stated by Kaur and Worgan but was higher than the one reported by Vorapreeda et al , With respect to the fatty acid composition, A. oryzae lipid had 25.79% PA, 11.18% stearic acid (C18:0, SA), 15.48% oleic acid (C18:1, OA), and 27.14% LA as the main fatty acids, as shown in Table .…”

Section: Resultsmentioning

confidence: 51%

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Efficient Strategy by a Newly Isolated Aspergillus oryzae to Harvest Chlorella vulgaris MBFJNU-1 from Original Swine Wastewater

Xie

Chi

et al. 2022

ACS Sustainable Chem. Eng.

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Biological approach is a promising method to efficiently harvest microalgal cells after the wastewater treatment by microalgae. This work aimed to develop an efficient bioflocculation process with a newly isolated filamentous fungus to harvest microalgal cells from the treatment of original swine wastewater (OSW) by Chlorella vulgaris MBFJNU-1 and primarily grope the individual mechanism for this bioflocculation. C. vulgaris MBFJNU-1 performed well growth and nutrient removal (total nitrogen, 72.29–90.98%; NH4 +-N, 97.18–97.80%; and total phosphorus, 47.51–70.77%) after the OSW treatment. Among the isolated filamentous fungi, Aspergillus oryzae exhibited a superior feature to harvest microalgal cells in the OSW treatment by microalga. Under the optimized conditions, the fungal pellet-assisted method with the newly isolated A. oryzae to harvest C. vulgaris cells achieved the highest bioflocculation efficiency (>90%). Moreover, the obtained results suggested that specific components on fungal pellet-derived cell walls belonged to glycoprotein, playing vital roles in the attachment of Chlorella cells. Furthermore, the fungal–microalgal biomass had around 27% lipid with over 90% C16 + C18, which was the promising feedstock for sustainable and renewable biodiesel production. Taken together, the bioflocculation process mediated with the newly isolated A. oryzae was a promising approach to harvest algal cells from wastewater treatment for sustainable biofuels.

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Section: T H Icontrasting

confidence: 75%

Section: Resultsmentioning

confidence: 51%

Efficient Strategy by a Newly Isolated Aspergillus oryzae to Harvest Chlorella vulgaris MBFJNU-1 from Original Swine Wastewater

Xie

Chi

et al. 2022

ACS Sustainable Chem. Eng.

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“…Typically, the key metabolite acetyl-CoA is independently synthesized by several metabolic pathways, which are available as reference pathways in the KEGG database. Although there are some reports that A. oryzae and R. oryzae contain lipids at high levels (Kaur & Worgan, 1982;Meng et al, 2009;Ratledge, 1986;Suzuki, 1991), not many efforts have been made to study the lipid physiology of these fungi, particularly in terms of lipid production. However, from the genome data that are currently available, we identified the missing enzymes that catalyse acetyl-CoA synthesis in the oleaginous strains, which were localized in the cytosol and mitochondria.…”

Section: Discussionmentioning

confidence: 99%

“…In this work, comparative genome analysis of nonoleaginous and oleaginous micro-organisms was performed to investigate candidate orthologous genes and enzymes related to oleaginicity. The oleaginous strains studied were selected based on their genome availability and oleagenicity features, and included Aspergillus oryzae (Kaur & Worgan, 1982;Lin et al, 2010;Meng et al, 2009;Santos-Fo et al, 2011), M. circinelloides (Vicente et al 2010), Rhizopus oryzae (Ratledge, 1986;Suzuki, 1991) and Y. lipolytica (Beopoulos et al, 2009), which have been shown to be potential lipid producers (Ageitos et al, 2011;Ratledge, 2004). We also emphasized the metabolism of acetyl-CoA, which is the key two-carbon metabolite in several metabolic processes, particularly in terms of precursor supply for fatty acid synthesis in oleaginous strains.…”

Section: Introductionmentioning

confidence: 99%

Alternative routes of acetyl-CoA synthesis identified by comparative genomic analysis: involvement in the lipid production of oleaginous yeast and fungi

et al. 2012

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For a bio-based economy, microbial lipids offer a potential solution as alternative feedstocks in the oleochemical industry. The existing genome data for the promising strains, oleaginous yeasts and fungi, allowed us to investigate candidate orthologous sequences that participate in their oleaginicity. Comparative genome analysis of the non-oleaginous (Saccharomyces cerevisiae, Candida albicans and Ashbya gossypii ) and oleaginous strains (Yarrowia lipolytica, Rhizopus oryzae, Aspergillus oryzae and Mucor circinelloides) showed that 209 orthologous protein sequences of the oleaginous microbes were distributed over several processes of the cells. Based on the 41 sequences categorized by metabolism, putative routes potentially involved in the generation of precursors for fatty acid and lipid synthesis, particularly acetyl-CoA, were then identified that were not present in the non-oleaginous strains. We found a set of the orthologous oleaginous proteins that was responsible for the biosynthesis of this key two-carbon metabolite through citrate catabolism, fatty acid b-oxidation, leucine metabolism and lysine degradation. Our findings suggest a relationship between carbohydrate, lipid and amino acid metabolism in the biosynthesis of acetyl-CoA, which contributes to the lipid production of oleaginous microbes. INTRODUCTIONLipids are dynamically bioactive molecules that contribute to the regulation of several complex systems of living cells. Besides the medical perspective, the remarkable growth of the lipid field is undoubtedly driven by the demand for feedstock for the oleochemical industry. In addition to the production of n-3 and n-6 polyunsaturated fatty acids that are beneficial to human health, extensive attention is being directed to biodiesel production from micro-organisms to replace non-sustainable petroleum (Liu & Zhao, 2007; Vicente et al., 2010). Certain strains, such as Rhodosporidiun toruloides, Lipomyces starkeyi, Yarrowia lipolytica and Mucor circinelloides, are known to accumulate substantial amounts of lipids, accounting for more than 20 % of their biomass, and are thus called oleaginous strains (Ageitos et al., 2011;Beopoulos et al., 2009;Meng et al. 2009;Ratledge, 2004). Due to their short cultivation time, their high level of intracellular lipids that are predominantly triacylglycerol (TAG) and their utilization of various substrates, oleaginous yeasts and fungi have become important model systems for alternatives to traditional sources of lipids derived from fossil, animal and plant origins (Beopoulos et al., 2009;Ratledge, 2004). Therefore, an understanding of lipid physiology is required to improve the efficient production of lipids of commercial interest. An integrated approach has been implemented using recent developments for studying the lipid metabolism of these promising micro-organisms. It has been reported that the lipid production of these oleaginous species is enhanced by controlling cultivation or nutritional conditions (Certik et al., 1999;Ruenwai et al., 2010). Based on the bioc...

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“…With the advent of new frontiers in biotechnology, the spectrum of amylase application has expanded into many other fields, such as clinical, medical and analytical chemistry (Forgarty, 1983;Ibukun and Akindumila, 1998;Achi & Njoku, 1992;Okolo et al, 1995 andPandey et al, 2000a&b). The pretreatment of the substrates from several fungi amylases were widely used in industry for production numerous products such as lipid production (Kaur and Worgan, 1982), organic acid like lactic acid (Huang et al, 2003& 2005Jin et al, 2003), production of fungal protein and amino acids (Jin et al, 1999), Ethanol (Gregg &Sadder, 1995 andBBI, 2002) and biodeterioration of industrial paper (Rojas et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Production of Amylase Enzymes by Filamentous Fungi

Darwish

Afifi

Mostafa

et al. 2009

Journal of Food and Dairy Sciences

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Fifty-two out of one hundred and forty-four isolates of filamentous fungi were recorded as amylase producer, but with different degrees, on solid plate method. Chemical constituents of potato waste samples (collected from the Chpis' Factory for Food Industries, Assiut, Egypt) were determined by chemical analysis. Twelve isolates (eight highly producer isolates and four isolates isolated from potato wastes) were screened for amylase production on potato wastes. Aspergillus flavus443 was recorded as the best enzyme producer on potato wastes and synthetic medium. The best environmental and nutritional conditions for amylase production by Aspergillus flavus443 were : [50 gm of potato wastes with 10 ml distilled water , manitol (1%) as a carbon source , casein (1%) as a nitrogen source , pH 5 and the medium was incubated at 40 ºC for three days). *R.s. = Residue of the reducing sugars in the culture medium (g/l) **M. d. c.z = Mean diameters of clearing zone(mm)

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Lipid production by Aspergillus oryzae from starch substrates

Cited by 10 publications

References 11 publications

Efficient Strategy by a Newly Isolated Aspergillus oryzae to Harvest Chlorella vulgaris MBFJNU-1 from Original Swine Wastewater

Efficient Strategy by a Newly Isolated Aspergillus oryzae to Harvest Chlorella vulgaris MBFJNU-1 from Original Swine Wastewater

Alternative routes of acetyl-CoA synthesis identified by comparative genomic analysis: involvement in the lipid production of oleaginous yeast and fungi

Production of Amylase Enzymes by Filamentous Fungi

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