Lipid Polyunsaturated Fatty Acid Chains in Mouse Kidneys Were Increased within 5 min of a Single High Dose Whole Body Irradiation

Abstract

To understand the ultra-early reaction of normal organ lipids during irradiation, we investigated the response of lipids, including polyunsaturated fatty acid (PUFA) chains, which are particularly susceptible to damage by ROS, in mice’s kidneys, lungs, brains, and livers within 5 min of single high-dose irradiation. In this study, we set up three groups of C56BL/6 male mice and conducted whole-body irradiation with 0 Gy, 10 Gy, and 20 Gy single doses. Kidney, lung, brain, and liver tissues were collected within 5 min of irradiation. PUFA-targeted and whole lipidomic analyses were conducted using liquid chromatography–tandem mass spectrometry (LC-MS/MS). The results showed that PUFA chains of kidney phosphatidylcholine (PC), phosphatidylethanolamine (PE), and triacylglycerol (TG) significantly increased within 5 min of 10 Gy and 20 Gy irradiation. The main components of increased PUFA chains in PC and PE were C18:2, C20:4, and C22:6, and in TG the main component was C18:2. The kidney lipidomes also showed significant changes from the perspective of lipid species, mainly dominated by an increase in PC, PE, TG, and signal lipids, while lipidomes of the lung, brain, and liver were slightly changed. Our results revealed that acute PUFA chains increase and other lipidomic changes in the kidney upon whole-body irradiation within 5 min of irradiation. The significantly increased lipids also showed a consistent preference for possessing PUFA chains. The lipidomic changes varied from organ to organ, which indicates that the response upon irradiation within a short time is tissue-specific.

Lipidomics of Microplasma-Irradiated Cells at Optimized Discharge Conditions for the Absorption of High-Molecule Drug

“…However, our analysis revealed a significant increase in total PUFA-containing PC species in B4K0H cells (Figure S1), indicating that peroxidation was not a major phenomenon in the irradiated cells. These findings resonate with a recent study by Li et al, who observed a notable increase in PUFA chains within kidney PC, PE, and TG species within 5 min of whole-body irradiation with 10 Gy and 20 Gy in mice [29].…”

Comparative analyses of adsorbed circulating proteins in the PMMA and PES hemodiafilters in patients on predilution online hemodiafiltration

“…LC-MS/MS is useful to compare the changes in serum protein profiles between low-flux and high-flux HD [22]. LC-MS/MS can assess the efficacy of protein removal from dialyzer membranes by analyzing toxic substances [23,24] and unintentionally lost proteins[25]. In this study, by using mass spectrometric analysis, we first showed that the PMMA membrane exhibited significantly higher adsorption of β2-MG, dermcidin, and immunoglobulin λ-1 light chain.…”

Ultrastructural Analysis of Large Japanese Field Mouse (Apodemus speciosus) Testes Exposed to Low-Dose-Rate (LDR) Radiation after the Fukushima Nuclear Power Plant Accident

“…Interestingly, increased lipid droplets were commonly found in radioresistant cancer cells as a stress response. Their presence was explained by a decrease in lipid peroxidation induced by ionizing radiation and accounts for an improved antioxidant ability due to higher ROS levels [54][55][56]. Data from previous studies have shown that stem spermatogonia were not killed at low-dose irradiation in mice and immediately began repopulating the seminiferous epithelium with different sperm cells [57].…”