Lipid phase transitions in model biomembranes

Chapman, D.; Peel, William E.; Kingston, Benjamin R.; Lilley, Terence H.

doi:10.1016/0005-2736(77)90002-5

Cited by 139 publications

(31 citation statements)

References 25 publications

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“…Within the experimental error, the phase behavior of the 10-(perfluorohexyl)-decanol-DPPC system did not depend on the solution employed to hydrate the mixtures (water, 0.15 M sodium chloride or 2 mM calcium chloride; data not shown). This observation is in agreement with previous studies that have shown these concentrations of sodium and calcium chloride have little or no effect on the macroscopic phase behavior of phosphatidylcholines such as DPPC [25][26][27][28]. We therefore only present the results from mixtures hydrated with the sodium chloride solution.…”

Section: Mixing Behavior In Fully Hydrated Bilayerssupporting

confidence: 91%

Mixing behavior of 10-(perfluorohexyl)-decanol and DPPC

Lehmler

Bummer

2005

Colloids and Surfaces B: Biointerfaces

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Fluorocarbon alcohol such as 10-(perfluorohexyl)-decanol are of interest for novel pulmonary drug delivery approaches. The purpose of this study was to investigate the mixing behavior of 10-(perfluorohexyl)-decanol with dipalmitoylphosphatidylcholine (DPPC), the major component of lung surfactant, as an aid in assessing usefulness for this and other biomedical applications. The impact of 10-(perfluorohexyl)-decanol on the phase transitions of DPPC bilayers fully hydrated with a 0.15 M sodium chloride solution were studied using differential scanning calorimetry (DSC). No peak corresponding to excess alcohol was observed. The fluorinated alcohol caused DPPC peak broadening, especially below X DPPC < 0.95, and elimination of the pretransition of DPPC at X DPPC ∼ 0.91. The onset of the main phase transition remains constant down to X DPPC ∼ 0.91, suggesting limited miscibility in the gel phase. Hydration of the 10-(perfluorohexyl)-decanol-DPPC mixtures with calcium chloride (2 mM) in place of sodium chloride did not alter the macroscopic phase behavior. In addition to the thermal properties, the miscibility of 10-(perfluorohexyl)-decanol in DPPC in monolayers at the air water interface was investigated on water, sodium chloride (0.15 M), calcium chloride (2 mM) or hydrochloric acid (pH=1.9) subphases. The concentration dependence of the onset pressure of the liquid-expanded to liquid condensed phase transition of DPPC showed a slight change with increasing mole fraction on all four subphases. The surface areamole fraction diagrams of 10-(perfluorohexyl)-decanol and DPPC on water, sodium chloride and calcium chloride showed near ideal behavior with slight negative deviations at higher surface pressure. A more significant negative deviation was observed for the hydrochloric acid subphase. Overall, both the DSC and the monolayer studies suggest that 10-(perfluorohexyl)-decanol and DPPC are partially miscible in biological mono-and bilayers. The macroscopic phase behavior 10-(perfluorohexyl)-decanol-DPPC system is significantly different from the analogous hydrocarbon system, which is attributed to a less favorable packing of the partially fluorinated hydrophobic tails in the mono-and bilayer.

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Section: Mixing Behavior In Fully Hydrated Bilayerssupporting

confidence: 91%

Mixing behavior of 10-(perfluorohexyl)-decanol and DPPC

Lehmler

Bummer

2005

Colloids and Surfaces B: Biointerfaces

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“…Thus, Na must predominate even if they have the same affinity to the binding site. Second, scanning differential calorimetry [31] and IR spectroscopy [9] experiments consistently suggested that the order of association between monovalent cations and phosphate groups follows Li > Na > K . Thus, Na is expected to more strongly interact with phosphate groups than K .…”

Section: Direct Imaging Of Lipid-ion Network Formation Under Physiolomentioning

confidence: 92%

Direct Imaging of Lipid-Ion Network Formation under Physiological Conditions by Frequency Modulation Atomic Force Microscopy

2007

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“…Curves of the dependence of surface potential on the concentration of adsorbing ion which exhibit a maximum in absolute values of surface potential (Figs 1, 2, 5, 6) provide the possibility of determining the parameters K i j and N j using [25,48,61,63 -651. (10) and (11) on the basis of curves 1-4 of Fig.…”

Section: Depicted Inmentioning

confidence: 99%

Binding of alkaline‐earth metal cations and some anions to hosphatidylcholine liposomes

Tatulian

1987

European Journal of Biochemistry

150

153

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The dependence of electrophoretic mobility of multilamellar liposomes composed of egg phosphatidylcholine (PtdCho), dimyristoyl-glycerophosphocholine (MyrzGro-P-Cho) and dipalmitoyl-glycerophosphocholine (Pam2-Gro-P-Cho) on the concentration of several cations and anions has been measured. Values of surface densities of binding sites and intrinsic binding constants of ions to liposome membranes were determined by processing the results in the framework of Gouy-Stern theory. Sharp reductions in the positive surface potential of Myr2Gro-P-Cho and Pam2Gro-P-Cho liposomes have been detected at the thermotropic transition of the lipids from the gel to liquid-crystalline phase. Similar alterations of liposome surface potential were revealed at the temperature of pretransitisn, as well as at about 50°C, in the case of Pam2Gro-P-Cho. A model is suggested for ion binding to PtdCho membranes, according to which the ion-binding sites are considered as point defects (vacancies) in the structure of lipid head-groups arranged over a trigonal lattice. Abbreviations. PtdCho, 1,2-diacyl-sn-glycero(3)phosphocholine; PtdSer, 1,2-diacyl-sn-glycero(3)-~-phosphoserine; PtdEtn, 1,2-diacylsn-glycero(3)phosphoethanolamine; PtdIns, 1,2-diacyl-sn-gIycero(3)-phospho( I )-L-myo-inositol ; PtdGro, 1,2-diacyl-sn-glycero(3)-phospho(1)-sn-glycerol; Myr2Gro-P-Cho, 1,2-dimyristoyl-sn-glycero(3)-phosphocholine; Pam2Gro-P-Cho, 1,2-dipalmitoyl-sn-glycero(3)-phosphocholine ; LauZGro-P-Cho, 1,2-dilauroyl-sn-glycero(3)phosphocholine; Ste2Gro-P-Cho, 1,2-distearoyl-sn-glycero(3)phosphocholine; OlezGro-P-Cho, 1,2-dioleoyl-sn-glycero(3)phosphocholine; PamOleGro-P-Cho, l-palmitoyl-2-oleoyl-sn-glycero(3)phosphocholine; Pam2Gro-P-Etn, 1,2-dipalmitoyl-sn-glycero(3)phosphoethanolamine; Pam,Gro-P-Ser, 1,2-dipalmitoyl-sn-glycero(3)-~-phosphoserine.

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Lipid phase transitions in model biomembranes

Cited by 139 publications

References 25 publications

Mixing behavior of 10-(perfluorohexyl)-decanol and DPPC

Mixing behavior of 10-(perfluorohexyl)-decanol and DPPC

Direct Imaging of Lipid-Ion Network Formation under Physiological Conditions by Frequency Modulation Atomic Force Microscopy

Binding of alkaline‐earth metal cations and some anions to hosphatidylcholine liposomes

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