Lipid Peroxide Formation and Membrane Damage in Endotoxin‐Poisoned Mice

Sakaguchi, Shuhei; Kanda, Norio; Hsu, Cheng‐Chin; Sakaguchi, Osamu

doi:10.1111/j.1348-0421.1981.tb00026.x

Cited by 75 publications

(37 citation statements)

References 45 publications

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“…Conjugated dienes, such as malondialdehyde and 4-hydroxy-2(E)-nonenal, are recognized markers of lipid peroxidation and tissue damage. 8,[27][28][29][30] In this study, all M haemolytica-inoculated calves had significantly greater CD concentrations in lung tissue 2 and 6 hours after inoculation, compared with salineinoculated calves. This result is in agreement with results of another study in which increased lipid peroxidation was detected after pulmonary administration of endotoxin.…”

Section: Discussionmentioning

confidence: 82%

Effects of the synthetic selectin inhibitor TBC1269 on tissue damage during acute Mannheimia haemolytica-induced pneumonia in neonatal calves

Radi¹,

Caverly²,

Dixon³

et al. 2001

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Objective—To determine effects of the selectin inhibitor TBC1269 on neutrophil-mediated pulmonary damage during acute Mannheimia haemolytica-induced pneumonia in newborn calves. Animals—Eighteen 1- to 3-day-old colostrumdeprived calves. Procedure—Mannheimia haemolytica or saline (0.9% NaCl) solution was inoculated in both cranial lung lobes of 12 and 6 calves, respectively. Calves were euthanatized 2 (saline, n = 3; M haemolytica, n = 4) or 6 hours (saline, n = 3; M haemolytica, n = 8) after inoculation. Four M haemolytica-inoculated calves euthanatized at 6 hours also received TBC1269 (25 mg/kg, IV) 30 minutes before and 2 hours after inoculation. Conjugated diene (CD) concentrations, inducible nitric oxide synthase (iNOS) expression, and apoptotic cell counts were determined in lung specimens collected during necropsy. Results—Conjugated diene concentrations were significantly increased in all M haemolytica-inoculated groups, compared with saline-inoculated groups. Calves treated with TBC1269 had decreased concentrations of CD, compared with untreated calves, although the difference was not significant. Number of apoptotic neutrophils and macrophages increased significantly in TBC1269-treated calves, compared with untreated calves. Inducible nitric oxide synthase was expressed by epithelial cells and leukocytes. However, iNOS was less abundant in airway epithelial cells associated with inflammatory exudates. Degree of iNOS expression was similar between TBC1269-treated and untreated calves. Conclusions—Mannheimia haemolytica infection in neonatal calves resulted in pulmonary tissue damage and decreased epithelial cell iNOS expression. The selectin inhibitor TCB1269 altered, but did not completely inhibit, neutrophil-mediated pulmonary damage. ( Am J Vet Res 2001;62:17–22)

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Section: Discussionmentioning

confidence: 82%

Effects of the synthetic selectin inhibitor TBC1269 on tissue damage during acute Mannheimia haemolytica-induced pneumonia in neonatal calves

Radi¹,

Caverly²,

Dixon³

et al. 2001

ajvr

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“…We reported previously, glutathione (GSH) reductase, and peroxidase activities and sulfhydryl level declined markedly in mouse liver by 18hr after endotoxin administration (14). Therefore, with endotoxin administration the disturbed metabolism of lipid peroxide by intramitochondrial GSH peroxidase may result in consumption of reduced GSH and in an increase in the NAD(P)+/NAD(P)H ratio (2).…”

mentioning

confidence: 99%

Calcium Behavior in Endotoxin‐Poisoned Mice: Especially Calcium Accumulation in Mitochondria

Sakaguchi¹,

Abe²,

Sakaguchi³

1984

Microbiology and Immunology

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A possible role of intracellular Ca2+ and participation of calmodulin in cellular metabolism in endotoxin‐poisoned mice were investigated. The levels of calcium in liver cytosol and liver mitochondria fractions in poisoned mice were markedly higher 18–48 hr after endotoxin injection than in the control mice. On the other hand, the levels of serum calcium in the poisoned mice were about 20% lower at 18 hr than in the controls. The serum calcium levels in mice injected with 50 and 100 μg of endotoxin showed no dose‐response effect, but a dose‐response effect was observed at a dose of 200–400 μg. The serum Ca2+ levels in endotoxin‐tolerant mice were similar to those in the control mice. The levels in mice injected with glucocorticoid‐antagonizing factor mice were about 14% lower at 3 hr than in the controls. The mice fed a vitamin D3‐ and calcium‐free diet showed a higher mortality rate in the early stage (12–18 hr) of endotoxication than that of the mice fed a normal diet.The lipid peroxide levels and Ca2+‐ATPase activity in the liver mitochondria fraction in endotoxin‐poisoned mice showed a higher level than those of the control mice. There was little or no difference in the levels of serum glucose between the mice injected with calmodulin antagonist (trifluoperazine, TFP) plus endotoxin and those given endotoxin alone. However, the liver glycogen levels in TFP plus endotoxin‐treated mice were markedly higher than that in mice given endotoxin alone. Furthermore, calcium antagonist (verapamil) plus endotoxin‐treated mice had about a 40% higher survival rate after 72 hr than those given endotoxin alone.The findings suggest that there is a possibility of participation of the Ca2+‐calmodulin system in carbohydrate metabolic disorders during endotoxemia and that the changes in intracellular Ca2+ may result in various metabolic disorders.

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“…Free radical injury occurs via lipid peroxidation in a variety of disease processes including shock. We reported previously 25,26) that endotoxin injection resulted in liver lipid peroxide formation and membrane damage in experimental animals, causing decreased levels of free radical scavengers or quenchers. In a previous study, 5) we also observed that Sho-saiko-to appears to protect the liver cell plasma membrane from injury by free radicals, which occurs in the state of tissue ischemia during endotoxemia.…”

Section: Discussionmentioning

confidence: 99%

Preventive Effects of a Traditional Chinese Medicine (Sho-saiko-to) on Septic Shock Symptoms; Approached from Heme Metabolic Disorders in Endotoxemia

Sakaguchi

Furusawa

Iizuka

2005

Biological & Pharmaceutical Bulletin

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Sho-saiko-to, one of the the most frequently prescribed Kampo medicines, is used to treat chronic hepatitis and has shown confirmed clinical efficacy. The present study was performed with respect to heme metabolism to study the preventive effects of Sho-saiko-to against endotoxemia. Endotoxin was injected intraperitoneally at a dose of 6 mg/kg into Sho-saiko-to (500 mg/kg/d, p.o.)-pretreated rats, and its administration clearly prevented the endotoxin-induced hypoferremia. In rats pretreated with Sho-saiko-to, the activity of hepatic d d-aminolevulinate synthetase and cytochrome P-450 level 18 h after endotoxin injection were significantly increased as compared to rats treated with endotoxin alone. Similarly, Sho-saiko-to significantly depressed the endotoxin-induced increase in heme oxygenase activity in liver microsomes. These findings suggested that the extent of shock syndrome caused by endotoxin may be due, at least in part, to changes in heme metabolic disturbance during endotoxemia. Sho-saiko-to may therefore protect rats against lethality caused by endotoxin through its ability to regulate the heme metabolism in septic shock.

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Lipid Peroxide Formation and Membrane Damage in Endotoxin‐Poisoned Mice

Cited by 75 publications

References 45 publications

Effects of the synthetic selectin inhibitor TBC1269 on tissue damage during acute Mannheimia haemolytica-induced pneumonia in neonatal calves

Effects of the synthetic selectin inhibitor TBC1269 on tissue damage during acute Mannheimia haemolytica-induced pneumonia in neonatal calves

Calcium Behavior in Endotoxin‐Poisoned Mice: Especially Calcium Accumulation in Mitochondria

Preventive Effects of a Traditional Chinese Medicine (Sho-saiko-to) on Septic Shock Symptoms; Approached from Heme Metabolic Disorders in Endotoxemia

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