Lipid peroxidation in the presence of iron oxides

Chouchane, Salem; Guignard, J.; Fournier, J.

doi:10.1080/02772240009358891

Cited by 6 publications

(8 citation statements)

References 24 publications

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“…Lipid peroxidation was determined by measurement of the concentration of thiobarturic acid-reactive substances (TBARS) in cell lysates (Chouchane et al, 2000). Briefly, cell lysates (0.45 ml) were mixed with 0.05 ml of 8.1% SDS, 0.08 ml of 4% BHT and 0.02 ml of 50 mM EDTA and then centrifuged at 10,000Âg for 10 min.…”

Section: Lipid Peroxidationmentioning

confidence: 99%

Alleviation of aflatoxin B1-induced oxidative stress in HepG2 cells by volatile extract from Allii Fistulosi Bulbus

et al. 2005

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Section: Lipid Peroxidationmentioning

confidence: 99%

Alleviation of aflatoxin B1-induced oxidative stress in HepG2 cells by volatile extract from Allii Fistulosi Bulbus

et al. 2005

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“…After cooling to room temperature, 3 mL of 1-butanol (99.5% in purity, Merck) were added, and the solution was centrifuged for 10 min (8,000 rpm), in order to extract the pink and yellow chromogens formed (TBA-MDA-TBA and TBA-monoaldehyde, respectively). The upper phase (butanol) containing colored chromogens was measured at 1=535 nm for TBA-MDA-TBA, 1=500 nm for TBAsaturated monoaldehyde, and 1= 450 nm for TBApolyunsaturated monoaldehyde [20,32] against the pure 1-butanol as a reference by UV-visible spectrometer (Jasco, V-550).…”

Section: Lipid Peroxidationmentioning

confidence: 99%

“…), able to attack a great variety of target molecules, and those with weak electrophilic character (iron super-oxo species, various oxygenated complexes of Fe 3+ , etc. ), only able to trigger lipid peroxidation [14,19,20]. The hydroxyl radical (HO•) is one of the strongest electrophilic ROS and is investigated in most reactivity studies.…”

Section: Introductionmentioning

confidence: 99%

Iron topochemistry and surface reactivity of amphibole asbestos: relations with in vitro toxicity

et al. 2011

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Chemical reactivity of asbestos tremolite from Italy and USA localities and Union Internationale Contre le Cancer (UICC) crocidolite was studied in relation to Fe content, oxidation state, and structural coordination. Direct correlation between amount of Fe(2+) at the exposed M(1) and M(2) sites of the amphibole structure and fiber chemical reactivity was established. The in vitro toxicity of the same samples was investigated on human alveolar A549 cell line. Relationship between crystal-chemical features and cell toxicity is not straightforward. UICC crocidolite has Fe content and chemical reactivity largely higher than that of tremolite samples, but all show comparable in vitro toxic potential. Results obtained evidenced that Fe topochemistry is not a primary factor for induced cell toxicity, though it accounts for asbestos chemical reactivity (and possibly genotoxicity).

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“…The peroxidation mechanism is very complex and implies the presence of ROS species which is revealed by the detection of some degradation products from this reaction [for example malonaldehyde (MDA), total monoaldehydes, etc.] (Halliwell and Gutteridge, 1986;Chouchane et al, 2000). MDA, known for its toxicity, is considered to be one of the main indices of peroxidation (Fournier et al, 1995).…”

Section: Samplesmentioning

confidence: 99%

“…The procedure for the detection of MDA was carried out from solids suspensions in aqueous and aerated medium, according to the same experimental protocol previously detailed (Chouchane et al, 2000).…”

Section: Samplesmentioning

confidence: 99%

In Vitro Cytotoxicity and Transforming Potential of Industrial Carbon Dust (Fibers and Particles) in Syrian Hamster Embryo (SHE) Cells

2010

The Annals of Occupational Hygiene

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Carbon fibers have many applications, mainly in high-tech industries such as the aviation industry. Eleven carbon samples (fibers and particles) coming from an aeronautic group were tested for their cytotoxicity and carcinogenic potential using in vitro short-term assays in Syrian hamster embryo cells. These samples were taken during each important step of the process, i.e. from the initial heating of polyacrylonitrile fibers to pure carbon fibers. They were compared to an asbestos fiber, an amorphous silica, and two commercial graphite powders. Their physical-chemical characteristics and their capacity to release reactive oxygen species (ROS) were determined. This study showed that none of the carbon samples was able to generate ROS as measured by Electron Paramagnetic Resonance analysis, and in our biological assays, they demonstrated no morphological transformation potential and low cytotoxicity compared to positive control (chrysotile asbestos).

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Lipid peroxidation in the presence of iron oxides

Cited by 6 publications

References 24 publications

Alleviation of aflatoxin B1-induced oxidative stress in HepG2 cells by volatile extract from Allii Fistulosi Bulbus

Alleviation of aflatoxin B1-induced oxidative stress in HepG2 cells by volatile extract from Allii Fistulosi Bulbus

Iron topochemistry and surface reactivity of amphibole asbestos: relations with in vitro toxicity

In Vitro Cytotoxicity and Transforming Potential of Industrial Carbon Dust (Fibers and Particles) in Syrian Hamster Embryo (SHE) Cells

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