Lipid peroxidation in the descending thoracic aorta of rats deprived of REM sleep using the inverted flowerpot technique

Nawi, Afifah; Eu, Koh Liew; Faris, Asma Nadia Ahmad; Ahmad, Wan Amir Nizam Wan; Noordin, Liza

doi:10.1113/ep088667

Cited by 11 publications

(16 citation statements)

References 48 publications

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“…For adaptation, each rat in REMsd, REMsd-H and TC groups was adapted individually in a dry tank with two inverted flowerpots ( 25 – 26 ), while each rat in the control group was placed individually in a standard dry cage for 72 h. The size of the tank used was 30 cm height, 30 cm length and 60 cm width. For REMsd and REMsd-H groups, two flowerpots (6.5 cm diameter) were placed in the dry tank.…”

Section: Methodsmentioning

confidence: 99%

“…The water prevented the rats from sleeping and the rats would get back on the platform. The inverted flowerpot technique had been utilised by other studies to achieve REM sleep deprivation ( 25 – 28 ). Food and drinks were provided throughout the adaptation and experiment.…”

Section: Methodsmentioning

confidence: 99%

“…The platforms used were larger in diameter (13.5 cm diameter) compared to REMsd and REMsd-H groups. The larger platforms were provided to ensure that this group of rats would be not REM sleep-deprived while examining the effect of exposure to the aquatic environment ( 25 – 26 ).…”

Section: Methodsmentioning

confidence: 99%

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Impact of Rapid Eye Movement Sleep Deprivation on Pain Behaviour and Oxidative Stress in the Thalamus: Role of Tualang Honey Supplementation

Shafie¹,

Aziz²,

Long³

et al. 2022

MJMS

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Background: Insufficient sleep alters the body’s physiological functions. This study investigated whether oxidative stress (OS) in the thalamus was correlated with the pain behaviour score in the rapid eye movement (REM) sleep-deprived rat model. Methods: Four groups of Sprague-Dawley rats were included in the study (n = 6): i) control; ii) REM sleep-deprived rats for 72 h (REMsd); iii) REM sleep-deprived rats for 72 h pretreated with Tualang honey (REMsd-H) and iv) tank control (TC). Following the intervention, 1% formalin was injected on the right hind paw and pain behaviour was recorded for 1 h. OS markers of glutathione (GSH), glutathione reductase (GR), superoxide dismutase (SOD), catalase (CAT) and malondialdehyde (MDA) in the thalamus were measured. Results: REM sleep deprivation increased pain behaviour scores in phase II of the formalin test with decreased GSH, GR, SOD and CAT. The MDA level was significantly higher in the REMsd compared to REMsd-H. There was an inverse correlation between pain behaviour scores and GSH, GR and SOD levels. A positive correlation was found between the pain behaviour score and the MDA level. Conclusion: OS levels were significantly correlated with the pain behaviour score in the REMsd rats. Tualang honey administration reduced pain behaviour score and OS in the thalamus.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Impact of Rapid Eye Movement Sleep Deprivation on Pain Behaviour and Oxidative Stress in the Thalamus: Role of Tualang Honey Supplementation

Shafie¹,

Aziz²,

Long³

et al. 2022

MJMS

Self Cite

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“…Sleep disorders related to inflammation and sympathetic activation lead to a change in blood vessel architecture, characterized by elastic fiber fracture and disorder, an increase in inflammatory cells, lipid peroxidation, inflammation, and sympathetic activation, which may induce endothelial dysfunction. Endothelial dysfunction is a critical factor in the increased risk of CVD [ 91 - 95 ]. In models of acute and chronic SD in healthy volunteers, Sauvet et al [ 96 , 97 ], Dettoni et al [ 98 ], measured biomarkers of microvascular reactivity and endothelial activation, elevated plasma E-selectin levels, and significantly increased sympathetic neural activity during SD.…”

Section: Mechanisms Of Chd Caused By Sdmentioning

confidence: 99%

Effects of sleep deprivation on coronary heart disease

Wei

Duan

Guo

2022

Korean J Physiol Pharmacol

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The presence of artificial light enables humans to be active 24 h a day. Many people across the globe live in a social culture that encourages staying up late to meet the demands of various activities, such as work and school. Sleep deprivation (SD) is a severe health problem in modern society. Meanwhile, as with cardiometabolic disease, there was an obvious tendency that coronary heart disease (CHD) to become a global epidemic chronic disease. Specifically, SD can significantly increase the morbidity and mortality of CHD. However, the underlying mechanisms responsible for the effects of SD on CHD are multilayered and complex. Inflammatory response, lipid metabolism, oxidative stress, and endothelial function all contribute to cardiovascular lesions. In this review, the effects of SD on CHD development are summarized, and SD-related pathogenesis of coronary artery lesions is discussed. In general, early assessment of SD played a vital role in preventing the harmful consequences of CHD.

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“…Previous animal studies had shown the link between REM sleep deprivation and endothelial dysfunction, an early indicator of CVD development. [13][14][15] Endothelial dysfunction can be defined as the reduced vasodilatory capacity or any changes that impact the vasoprotective homeostatic function of the endothelium. 16 The dysfunction could be due to the damage to the vascular endothelium.…”

Section: Introductionmentioning

confidence: 99%

Oxidative Stress Induces Endothelial Dysfunction and Endothelial Cell Damage in Rapid Eye Movement (REM) Sleep Deprivation Animal Model

Noordin

Nawi

Zin

et al. 2022

imjm

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INTRODUCTION: Rapid eye movement (REM) sleep deprivation causes oxidative stress, leading to endothelial dysfunction, a predictor of cardiovascular disease. It is still unclear what causes endothelial dysfunction in patients with sleep disorder. This study evaluates the effects of REM sleep deprivation on the endothelium in the rat model of REM sleep deprivation. MATERIALS AND METHODS: Sprague-Dawley male rats (N=28) were divided into four groups (n=7); (1) free-moving control (FMC), (2) REM sleep deprivation (REMsd), (3) tank control (TC), and (4) sleep recovery (SR). The inverted flowerpot technique was utilised to develop REM sleep deprivation. Bodyweight gain (BWg), food consumption (Fc), and systolic blood pressure (SBP) were evaluated. The descending thoracic aorta was isolated to assess oxidative stress markers, in vitro functional study, and histomorphological examination. RESULTS: REM sleep deprivation showed a decrease in BWg significantly despite a significant increase in Fc, increased SBP, increased oxidative stress markers, caused endothelial dysfunction and endothelial cell damage. In REM sleep-deprived rats, there was a significant reduction in antioxidant markers, including total antioxidant capacity (TAC), superoxide dismutase (SOD) activity, catalase (CAT), and glutathione (GSH), while the levels of malondialdehyde (MDA) were significantly increased. The REM sleep-deprived rats displayed altered vascular function, including impaired vasorelaxation and hypercontractility. Histomorphology of the endothelium in REM sleep-deprived rats revealed features of endothelial damage. CONCLUSION: REM sleep deprivation is suggested to be linked to endothelial dysfunction due to endothelial damage. These changes are proposed to result from increased oxidative stress. Sleep recovery reduced the harmful effects following REM sleep deprivation.

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Lipid peroxidation in the descending thoracic aorta of rats deprived of REM sleep using the inverted flowerpot technique

Cited by 11 publications

References 48 publications

Impact of Rapid Eye Movement Sleep Deprivation on Pain Behaviour and Oxidative Stress in the Thalamus: Role of Tualang Honey Supplementation

Impact of Rapid Eye Movement Sleep Deprivation on Pain Behaviour and Oxidative Stress in the Thalamus: Role of Tualang Honey Supplementation

Effects of sleep deprivation on coronary heart disease

Oxidative Stress Induces Endothelial Dysfunction and Endothelial Cell Damage in Rapid Eye Movement (REM) Sleep Deprivation Animal Model

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