Lipid peroxidation in presence of ebselen

Batna, Andreas; Fuchs, Claus; Spiteller, Gerhard

doi:10.1016/s0009-3084(97)00037-6

Cited by 16 publications

(7 citation statements)

References 36 publications

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“…Ebselen is a synthetic, lipid‐soluble seleno‐organic compound having potent antioxidant capacity. It is also a novel anti‐inflammatory agent having glutathione peroxidase‐like activity [14–18]. It has therapeutic activity in neurological disorders, acute pancreatitis, noise‐induced hearing loss, and cardiotoxicity.…”

Section: Introductionmentioning

confidence: 99%

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Ebselen induces reactive oxygen species (ROS)‐mediated cytotoxicity in Saccharomyces cerevisiae with inhibition of glutamate dehydrogenase being a target

et al. 2014

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Ebselen is a synthetic, lipid-soluble seleno-organic compound. The high electrophilicity of ebselen enables it to react with multiple cysteine residues of various proteins. Despite extensive research on ebselen, its target molecules and mechanism of action remains less understood. We performed biochemical as well as in vivo experiments employing budding yeast as a model organism to understand the mode of action of ebselen. The growth curve analysis and FACS (florescence activated cell sorting) assays revealed that ebselen exerts growth inhibitory effects on yeast cells by causing a delay in cell cycle progression. We observed that ebselen exposure causes an increase in intracellular ROS levels and mitochondrial membrane potential, and that these effects were reversed by addition of antioxidants such as reduced glutathione (GSH) or N-acetyl-l-cysteine (NAC). Interestingly, a significant increase in ROS levels was noticed in gdh3-deleted cells compared to wild-type cells. Furthermore, we showed that ebselen inhibits GDH function by interacting with its cysteine residues, leading to the formation of inactive hexameric GDH. Two-dimensional gel electrophoresis revealed protein targets of ebselen including CPR1, the yeast homolog of Cyclophilin A. Additionally, ebselen treatment leads to the inhibition of yeast sporulation. These results indicate a novel direct connection between ebselen and redox homeostasis.

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Section: Introductionmentioning

confidence: 99%

“…It has therapeutic activity in neurological disorders, acute pancreatitis, noise‐induced hearing loss, and cardiotoxicity. It also exhibits antiatherosclerotic, antithrombotic, and cytoprotective properties [15,19–21]. However, excessive amounts of ebselen are toxic to the cells.…”

Section: Introductionmentioning

confidence: 99%

Ebselen induces reactive oxygen species (ROS)‐mediated cytotoxicity in Saccharomyces cerevisiae with inhibition of glutamate dehydrogenase being a target

et al. 2014

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“…Increased lipoperoxidation, as observed by the increase in TBARS concentration and the decrease in NPSH concentration in the erythrocytes may reflect a possible decrease in GSH concentration, a tripeptide that is key in protecting erythrocytes from oxidative damage. 15,17,[31][32][33] The pyrrolic metabolites produced from PAs have been wellstudied, and are capable of binding with GSH to form GSH conjugates. 6 Over 90% of GSH in the systemic circulation results from influx of this peptide from the liver.…”

Section: Discussionmentioning

confidence: 99%

“…15,16 Previous studies have described an increase in the osmotic fragility of the erythrocytes in experimental models of oxidative stress. 15,17,18 The damage to the antioxidant system or the increase in ROS production is responsible for alterations in the cellular structure of the erythrocytes. 14 It is known that an imbalance in the oxidant-antioxidant status of the cell is associated with oxidative stress, and that it causes a cascade of events within the cell, such as lipid peroxidation and protein oxidation, 14,19 which might be the cause of hemolysis and anemia.…”

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confidence: 99%

Oxidative stress in the erythrocytes of cattle intoxicated withSeneciosp

Bondan¹,

Soares²,

Cecim³

et al. 2005

Veterinary Clinical Pathol

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“…Ebselen inhibits the Mpro with an IC 50 of 0.67 μM and exhibits antiviral activity with IC 50 at 4.67 μM. Ebselen (PZ51, DR-3305), a glutathione peroxidase mimic, has also been reported to function as antioxidant and antiinflammatory agent with therapeutic potential in neurological disorders, noise-induced hearing loss, cancers acute pancreatitis, mania and hypomania [17][18][19][20][21][22][23][24][25][26]. Ebselen also inhibits other viral cysteine proteases including SARS-CoV-2 papain-like protease and 2Apro or 3Cpro from enterovirus A71 (EV-A71) and EV-D68, indicating that ebselen is a multi-target drug candidate [27].…”

Section: Introductionmentioning

confidence: 99%