Lipid peroxidation in microsomes induced by crocidolite fibres

Gulumian, M.; Sardianos, Fay; Kilroe-Smith, T.A.; Ockerse, Gerald

doi:10.1016/0009-2797(83)90133-3

Cited by 43 publications

(18 citation statements)

References 18 publications

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“…In previous studies asbestos has been shown to induce lipid peroxidation with red blood cells (Gabor & Anca, 1975), phospholipid emulsions (Weitzman & Weitberg, 1985) and isolated microsomes (Gulumian et at., 1983). Others have shown the iron component of crocidolite fibres can catalyse the generation of hydroxyl radicals from hydrogen peroxide (Weitzman & Graceffa, 1984).…”

Section: Discussionmentioning

confidence: 99%

The role of catalytic iron in asbestos induced lipid peroxidation and DNA-strand breakage in C3H10T1/2 cells

Turver

Brown²

1987

Br J Cancer

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Summary The involvement of catalytic iron in the vitro activities of crocidolite asbestos has been investigated. Exposure of C3HlOT1 cells to either the UICC crocidolite standard reference sample or a non fibrous (milled) derivative resulted in an increase of thiobarbituric acid reactive substances. This catalytic activity was inhibited by pretreatment with the iron chelator desferrioxamine. The effect of this activity on cellular DNA was measured in an assay based on the production of DNA-strand breaks. Increased levels of DNA-strand breaks were detected in cultures treated with both the milled and UICC crocidolite. Inclusion of desferrioxamine with the asbestos inhibited DNA-strand breakage. It is concluded the catalytic iron present on the dust is capable of damaging both lipid and DNA and that this could be an important mechanism in asbestos pathogenicity.

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Section: Discussionmentioning

confidence: 99%

The role of catalytic iron in asbestos induced lipid peroxidation and DNA-strand breakage in C3H10T1/2 cells

Turver

Brown²

1987

Br J Cancer

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“…There are several hints that iron components in asbestos might play an important role in the causation of mesotheliomas (Churg & Warnock, 1981;McCaughey et al, 1985;Weitzman & Weitberg, 1985;Jones & Brachet, 1987). Furthermore, several studies report that asbestos induces free radical generation, lipid peroxidation and DNA damage (Gulumian et al, 1983;Kasai & Nishimura, 1984;Weitzman & Graceffa, 1984;Weitzman & Weitberg, 1985;Gulumian & Kilroe-Smith, 1987). Iron plays a major role in catalysing free radical production in general (Halliwell & Gutteridge, 1984;Aust et al, 1985;Girotti, 1985).…”

Section: Discussionmentioning

confidence: 99%

Induction of mesothelioma by intraperitoneal injections of ferric saccharate in male Wistar rats

Okada

Hamazaki²,

Toyokuni³

et al. 1989

Br J Cancer

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Summary Iron appears to play a major role in catalysing free radical production, leading to lipid peroxidation and DNA damage. We, therefore, investigated the effect of colloidal iron deposited in the peritoneum. Wistar male rats were given either ferric saccharate, ferric saccharate and nitrilotriacetic acid (NTA), NTA or saline. NTA was shown previously to 'free' iron to promote lipid peroxidation and an iron chelate of NTA is known to be carcinogenic to the kidney. Iron at a dose of 5 mg kg-' day-', and saline at a dose of 0.5 ml day-' were injected i.p. for 3 months. NTA at a dose of 83.5 mg kg-' day-' was give i.p for 5 months. All the rats were killed about a year later for histological examination. In nine of the 19 rats treated with ferric saccharate, mesothelial tumors were induced in the serosa of the tunica vaginalis or the length of the spermatic cord. Among rats treated with ferric saccharate and NTA, seven had localised mesotheliomas in the above locations and six had wide-spread peritoneal mesotheliomas. No mesothelial tumors developed in either NTA treated or saline treated rats. No pleural mesotheliomas were found in any group. These findings add to the evidence that iron is involved in some carcinogenic processes.

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“…This is not limited to internalization of fibers and has been studied in bacteria as well as in phagocytic cells (112)(113)(114). Phagocytosis is associated with lipid peroxidation, as demonstrated by several authors in cell-free systems (31,32,115,116) and in mouse fibroblasts (55) and macrophages (117 (53) report that the formation (119,120) and macrophages.…”

Section: Chromosome Damagementioning

confidence: 97%

Mechanisms of fiber-induced genotoxicity.

Jaurand

1997

Environ Health Perspect

117

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The mechanisms of particle-induced genotoxicity have been investigated mainly with asbestos fibers. The results are summarized and discussed in this paper. DNA damage can be produced by oxidoreduction processes generated by fibers. The extent of damage yield depends on experimental conditions: if iron is present, either on fibers or in the medium, damage is increased. However, iron reactivity does not explain all the results obtained in cell-free systems, as breakage of plasmid DNA was not directly associated with the amount of iron released by the fibers. The proximity of DNA to the site of generation of reactive oxygen species (ROS) is important because these species have an extremely short half-life. Damage to cellular DNA can be produced by oxidoreduction processes that originate from cells during phagocytosis. Secondary molecules that are more stable than ROS are probably involved in DNA damage. Oxidoreduction reactions originating from cells can induce mutations. Genotoxicity is also demonstrated by chromosomal damage associated with impaired mitosis, as evidenced by chromosome missegregation, spindle changes, alteration of cell cycle progression, formation of aneuploid and polyploid cells, and nuclear disruption. In some of these processes, the particle state and fiber dimensions are considered important parameters in the generation of genotoxic effects.

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Lipid peroxidation in microsomes induced by crocidolite fibres

Cited by 43 publications

References 18 publications

The role of catalytic iron in asbestos induced lipid peroxidation and DNA-strand breakage in C3H10T1/2 cells

The role of catalytic iron in asbestos induced lipid peroxidation and DNA-strand breakage in C3H10T1/2 cells

Induction of mesothelioma by intraperitoneal injections of ferric saccharate in male Wistar rats

Mechanisms of fiber-induced genotoxicity.

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