LIPID PEROXIDATION AND OTHER MEMBRANE DAMAGE PRODUCED IN <i>Escherichia coli</i> K1060 BY NEAR‐UV RADIATION AND DEUTERIUM OXIDE

Chamberlain, Joshua D.; Moss, S. H.

doi:10.1111/j.1751-1097.1987.tb07389.x

Cited by 80 publications

(39 citation statements)

References 19 publications

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“…Also a reanalysis of all samples (exposed but unstained) after 48 h showed identical results, which indicates that the degree of permeabilization was permanent and could not be repaired (data not shown). These damages are in line with earlier reports of lipid peroxidation and other membrane damages caused by near-UV light (9,33). Therefore, with the dye combination of SYTO9 and PI, one can detect both outer as well as inner membrane permeabilization in gram-negative bacteria.…”

Section: Discussionsupporting

confidence: 74%

Assessment and Interpretation of Bacterial Viability by Using the LIVE/DEAD BacLight Kit in Combination with Flow Cytometry

Berney

Hammes

Bosshard

et al. 2007

Appl Environ Microbiol

765

588

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The commercially available LIVE/DEAD BacLight kit is enjoying increased popularity among researchers in various fields of microbiology. Its use in combination with flow cytometry brought up new questions about how to interpret LIVE/DEAD staining results. Intermediate states, normally difficult to detect with epifluorescence microscopy, are a common phenomenon when the assay is used in flow cytometry and still lack rationale. It is shown here that the application of propidium iodide in combination with a green fluorescent total nucleic acid stain on UVA-irradiated cells of Escherichia coli, Salmonella enterica serovar Typhimurium, Shigella flexneri, and a community of freshwater bacteria resulted in a clear and distinctive flow cytometric staining pattern. In the gram-negative bacterium E. coli as well as in the two enteric pathogens, the pattern can be related to the presence of intermediate cellular states characterized by the degree of damage afflicted specifically on the bacterial outer membrane. This hypothesis is supported by the fact that EDTA-treated nonirradiated cells exhibit the same staining properties. On the contrary, this pattern was not observed in gram-positive Enterococcus faecalis, which lacks an outer membrane. Our observations add a new aspect to the LIVE/DEAD stain, which so far was believed to be dependent only on cytoplasmic membrane permeability.

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Section: Discussionsupporting

confidence: 74%

Assessment and Interpretation of Bacterial Viability by Using the LIVE/DEAD BacLight Kit in Combination with Flow Cytometry

Berney

Hammes

Bosshard

et al. 2007

Appl Environ Microbiol

765

588

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“…However, the presence of polyunsaturated lipids is a prerequisite for a peroxidative chain reaction, and most bacterial membranes lack these lipids (Imlay, 2009). Fenton reactions are accelerated by light (so called photo-Fenton reactions), and therefore radical damage speeds up in the light (Chamberlain & Moss, 1987). In E. coli the damaging effect of UVA light on membranes has been described previously (Moss & Smith, 1981), and the breakdown of energy metabolism was suggested as a cause for the cellular die-off in this enterobacterium (Berney et al, 2006a;Bosshard et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

The respiratory chain is the cell's Achilles' heel during UVA inactivation in Escherichia coli

et al. 2010

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Solar disinfection (SODIS) is used as an effective and inexpensive tool to improve the microbiological quality of drinking water in developing countries where no other means are available. Solar UVA light is the agent that inactivates bacteria during the treatment. Damage to bacterial membranes plays a crucial role in the inactivation process. This study showed that even slightly irradiated cells (after less than 1 h of simulated sunlight) were strongly affected in their ability to maintain essential parts of their energy metabolism, in particular of the respiratory chain (activities of NADH oxidase, succinate oxidase and lactate oxidase were measured). The cells' potential to generate ATP was also strongly inhibited. Many essential enzymes of carbon metabolism (glucose-6-phosphate dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase, lactate dehydrogenase and malate dehydrogenase) and defence against oxidative stress (catalases and glutathione-disulfide reductase) were reduced in their activity during SODIS. The work suggests that damage to membrane enzymes is a likely cause of membrane dysfunction (loss of membrane potential and increased membrane permeability) during UVA irradiation. In this study, the first targets on the way to cell death were found to be the respiratory chain and F 1 F 0 ATPase.

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“…Membrane lipid unsaturated acyl chains (Klamen and Tuveson, 1982;Dearden et al, 1985;Chamberlain and Moss, 1987), sterols (Girotti, 1985), and the sulfhydryl groups (Murphy, 1984;Moan and Vistnes, 1986) and aromatic amino acids (Walrant and Santus, 1974;Caldwell, 1987) of membrane proteins may be susceptible to direct UV-8-induced photooxidation or indirect degradative processes mediated by endogenous photosensitizers, free radicals, and other re-* Fax 1-301-504-7521.…”

Section: ~~ ~ ~ ~mentioning

confidence: 99%

Ultraviolet-Induced Photodegradation of Cucumber (Cucumis sativus L.) Microsomal and Soluble Protein Tryptophanyl Residues in Vitro

Caldwell

1993

Plant Physiol.

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l h e in vitro effects of ultraviolet B (280-320 nm) radiation on microsomal membrane proteins and partially purified ribulose bisphosphate carboxylase (Rubisco) from cucumber (Cucumis sativus l.) was investigated by measuring the direct photolytic reduction of tryptophan fluorescence and the formation of fluorescent photooxidation products. Exposure of microsomes and Rubisco to monochromatic 300-nm radiation resulted in the loss of intrinsic tryptophan fluorescence and the production of blue-emitting fluorophores. l h e major produd of tryptophan photolysis was tentatively identified as N-formylkynurenine (N-FK). Even though the rates of tryptophan photodegradation and N-FK formation were similar, the amount of blue fluorescence produced was significantly higher in the microsomes relative to Rubisco. Studies with various free radical scavengers and other modifiers indicated that tryptophan photodegradation requires oxygen and that the subsequent formation of N-FK may involve readive oxygen species. l h e optimum wavelengths for loss of typtophan fluorescence were 290 nm for the microsomes and 280 nm for Rubisco. The temperature dependence of tryptophan fluorescence and rate of tryptophan photodegradation indicated an alteration in the cucumber microsoma1 membranes at about 24"C, which influenced protein structure and tryptophan photosensitivity.

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LIPID PEROXIDATION AND OTHER MEMBRANE DAMAGE PRODUCED IN Escherichia coli K1060 BY NEAR‐UV RADIATION AND DEUTERIUM OXIDE

Cited by 80 publications

References 19 publications

Assessment and Interpretation of Bacterial Viability by Using the LIVE/DEAD BacLight Kit in Combination with Flow Cytometry

Assessment and Interpretation of Bacterial Viability by Using the LIVE/DEAD BacLight Kit in Combination with Flow Cytometry

The respiratory chain is the cell's Achilles' heel during UVA inactivation in Escherichia coli

Ultraviolet-Induced Photodegradation of Cucumber (Cucumis sativus L.) Microsomal and Soluble Protein Tryptophanyl Residues in Vitro

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