Lipid peroxidation and loss of potassium from red blood cells produced by phototoxic quinolones

Cárdenas, Maribel; Cortés, Magdalena P.; Fernandez, Béatrice; Peña, Wenceslao

doi:10.1016/0300-483x(92)90108-q

Cited by 9 publications

(2 citation statements)

References 27 publications

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“…Regardless of the molecular nature of this conductance, as a consequence of its activation by either UV light or free radical stress, cations can enter the cell, leading to membrane depolarization and increases in cytosolic calcium concentration. Thus, the present data can account for several of the primary pathological events observed after free radical stress such as potassium loss (Cardenas et al 1992), cell swelling due to sodium influx (Koliwad et al 1996 b ) and cytoplasmic free calcium increase (Kimura et al 1992). Excessive and/or persistent activation of this conductance might eventually cause cell swelling and ultimately lead to cell death.…”

Section: Discussionmentioning

confidence: 91%

“…From these studies it is evident that excessive UV light in the near‐visible range acts in a similar way to ionizing radiation and oxidative stress, evoking numerous cellular responses that may range from mild to severe erythema to malignant transformation of dermal tissues. At the cellular level, the most important macroscopic effects observed after UV irradiation appear to be lipid peroxidation (Morliere, Moysan, Santus, Huppe, Maziere & Dubertret, 1991; Beer, Olvey, Miller, Thomas & Godar, 1993), increases in intracellular calcium concentration (Kimura, Maeda & Hayashi, 1992), loss of potassium (Cardenas, Cortes, Fernandez & Pena, 1992) and activation of protein kinases (Devary, Gottlieb, Smeal & Karin, 1992; Kasid, Suy, Dent, Ray, Whiteside & Sturgill, 1996; Verheij et al 1996). The most severe consequences are cell swelling due to sodium influx (Koliwad, Kunze & Elliott, 1996 b ) and immediate apoptotic cell death (Beer et al 1993; Godar & Lucas, 1995).…”

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confidence: 99%

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Near‐visible ultraviolet light induces a novel ubiquitous calcium‐permeable cation current in mammalian cell lines

Mendez

Penner

1998

The Journal of Physiology

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1. We studied the immediate and short-term effects of UV light in the near-visible range at the cellular and membrane level using the whole-cell patch-clamp technique in combination with digital fluorescence imaging. 2. Illumination with monochromatic UVA light (340-380 nm) induced a sustained non-saturable increase in membrane conductance dependent on wavelength and light intensity in several different mammalian cell types including RBL, mast, HEK, PC12 and 3T3 cells. 3. The current was non-selective for cations and permeable to Ca2+, but was inhibited by trivalent cations and was not due to the activation of an endogenous ion channel. We termed this novel current ILiNC for light-induced non-selective cation current. 4. A similar current was evoked by chemical peroxidants such as hydrogen peroxide and tertbutylhydroperoxide, but not by cytosolic oxidized glutathione. 5. The free-radical scavengers tocopherol (vitamin E) and ascorbic acid (vitamin C) significantly reduced the UV light effect. 6. The generation of the current was membrane delimited since it could be induced by the same UVA treatment in cell-free membrane patches showing a similar wavelength dependence. 7. These results suggest that ILiNC is activated by UVA light-induced generation of free radicals acting through lipid or protein peroxidation, and may represent a ubiquitous mechanism by which Na+ and Ca2+ can enter cells after phototoxic or free radical-induced membrane damage.

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Section: Discussionmentioning

confidence: 91%

mentioning

confidence: 99%

Near‐visible ultraviolet light induces a novel ubiquitous calcium‐permeable cation current in mammalian cell lines

Mendez

Penner

1998

The Journal of Physiology

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The Modulation of Membrane Ion Movements by Cholesterol

Liu

Pierce

1994

Membrane Physiopathology

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Essential metal ions alter the lactoferrin binding to the erythrocyte plasma membrane receptors

Taleva

Maneva

Sirakov

1999

Biol Trace Elem Res

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The effect of metal ions at a concentration of 10(-8) to 10(-5) M [using their salts: ZnCl2, CdCl2, LiCl, CuSO4, NiSO4, Al2(SO4)3, (NH4)2MoO4 on the lactoferrin (Lf) binding to the erythrocyte membrane receptors was studied. In the absence of metal ions, Scatchard's analysis showed the existence of two kinds of binding site: one with high affinity and low capacity, and the another with low affinity and high capacity. All these metals, excluding Zn2+ and Cd2+, at a concentration 10(-5) M decreased the affinity of Lf binding (Ka1) to the high-affinity receptors. In the presence of Zn2+ and Cd2+, only the low-affinity binding site was found. Significant inhibition on the affinity (Ka2) of the low-affinity class of receptors showed Zn2+, Al3+, and Mo6+. Depending on their concentration (10(-8)-10(-5) M), these ions enhanced to a different extent, the binding capacity of the both types receptors, but the effect did not correspond to the applied doses. Several explanations of the mechanism for influence of the metal ions on the Lf-receptor interaction is discussed.

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Lipid peroxidation and loss of potassium from red blood cells produced by phototoxic quinolones

Cited by 9 publications

References 27 publications

Near‐visible ultraviolet light induces a novel ubiquitous calcium‐permeable cation current in mammalian cell lines

Near‐visible ultraviolet light induces a novel ubiquitous calcium‐permeable cation current in mammalian cell lines

The Modulation of Membrane Ion Movements by Cholesterol

Essential metal ions alter the lactoferrin binding to the erythrocyte plasma membrane receptors

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