Lipid Peroxidation and Antioxidative Enzyme Activity in Erythrocytes of Workers Occupationally Exposed to Aluminium

Bulat, Petar; Potkonjak, Biljana; Đujić, Ivana

doi:10.2478/10004-1254-59-2008-1853

Cited by 13 publications

(15 citation statements)

References 29 publications

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“…These results are in accordance with our previous results on human erythrocytes (22) and with the data of Bulat et al (42).…”

Section: Discussionsupporting

confidence: 95%

“…Recently (22), we reported that human erythrocytes exposed to aluminum undergo lipid peroxidation as well as reduction in the activity of erythrocyte antioxidant enzymes (superoxide dismutase, catalase and glutathione peroxidase). A reduced activity of antioxidant enzymes following Al exposure has been reported also in workers occupationally exposed to aluminum (42). Free radical scavengers and chelators of aluminium lower ROS levels in brain cells.…”

Section: Discussionmentioning

confidence: 95%

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Plasma and mitochondrial membrane perturbation induced by aluminum in human peripheral blood lymphocytes

Skarabahatava

Lukyanenko

Slobozhanina

et al. 2015

Journal of Trace Elements in Medicine and Biology

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“…These results are in accordance with our previous results on human erythrocytes (22) and with the data of Bulat et al (42).…”

Section: Discussionsupporting

confidence: 95%

Section: Discussionmentioning

confidence: 95%

Plasma and mitochondrial membrane perturbation induced by aluminum in human peripheral blood lymphocytes

Skarabahatava

Lukyanenko

Slobozhanina

et al. 2015

Journal of Trace Elements in Medicine and Biology

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“…However, according to the labels, the studied workers were exposed to toluene, xylene, ethylbenzene, butyl and ethyl acetate, acetone, methyl isobutyl ketone, titanium, aluminum, lead, cobalt, and chromium. It is already known that these compounds can induce DNA damages [15,[33][34][35] and an increase in oxidative stress chromosome rearrangement, and the NDI index is a biomarker of the proliferative status of the viable cell fraction [22]. An increase in NBUD frequency was observed in binucleated lymphocytes of the exposed group in Monday samples compared to Monday samples of the control group (Table 5), suggesting chromosomal damage and/or a DNA instability status in this group [22].…”

Section: Discussionmentioning

confidence: 86%

Occupational risk assessment of oxidative stress and genotoxicity in workers exposed to paints during a working week

Cassini

Calloni

Bortolini

et al. 2011

International Journal of Occupational Medicine and Environmental Health

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Objectives: Paints are complex mixtures of solvents and metals that can induce health damages in workers exposed to them. The aim of the present work was to evaluate possible oxidative and genotoxic effects in workers exposed to paints. Material and Methods: Peripheral blood and buccal cell samples were collected from 33 workers exposed to paints and 29 non-exposed workers (controls) during an ordinary working week (Monday morning and Friday evening). Oxidative markers were assessed using thiobarbituric acid assay, carbonylated proteins, superoxide dismutase and catalase activities. Hippuric acid and delta-aminolevulinic acid were determined as biomarkers of toluene and lead exposure, respectively. Genotoxicity was measured through comet assay and micronucleus (MN) frequencies. Results: The exposed group showed higher hippuric acid and delta-aminolevulinic acid levels (Friday samples) and lower superoxide dismutase activity (Monday samples) in relation to control group. DNA damage index (comet assay) was higher in the exposed group, both in Monday and Friday samples, compared to the control group. No differences were observed in frequency of micronuclei (MN) between the groups, either in lymphocytes or buccal cells. However, the exposed group presented an increase (Monday samples) in nuclear buds frequency in lymphocytes -a marker of gene amplificationas well as an increase in condensed chromatin in the buccal cells (Monday and Friday samples), suggesting induction of apoptosis. Furthermore, a decrease in the nuclear division index (Friday samples) was observed in the exposed group, indicating that paint exposure induces cytostatic effects in lymphocytes. Conclusion: The results suggest that individuals exposed to paints have increased levels of DNA damage.

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“…Measurement of (8-OHdG) is a highly sensitive assay for oxygen free radical damage to DNA (Canakci et al 2009;Valavanidis et al 2009). This biomarker has been used to estimate the DNA damage in humans after exposure to cancer-causing agents, such as heavy metals, and polycyclic aromatic hydrocarbons (Bulat et al 2008).…”

Section: Discussionmentioning

confidence: 99%

Assessment of Hematotoxicity and Genotoxicity among paint Workers in Assiut Governorate: a case control study

Maksoud

Aal

Ghandour

et al. 2018

Egypt J Forensic Sci

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Background: Occupational exposures to hazardous chemicals in paints cause serious health hazards in painters. The present study was designed to evaluate the possibility of hematotoxic and genotoxic effects of paint chemicals among painters in Assiut Governorate, Egypt. In addition the role of oxidative stress and apoptosis in mechanism of such toxic effects were studied. Methods: A case control study was performed on 50 male painters and 50 non-exposed healthy subjects, who were included as a control group after informed consent. Venous blood samples were obtained and analyzed for determination of total and differential blood count as hematotoxic markers as well as serum malondialdehyde (MDA) as an oxidant stress markers and total antioxidant enzymes. In addition, human B-cell lymphoma-2 (Bcl-2), caspase-3 and 8-hydroxyguanosine (8-OHdG) were assayed as markers of apoptosis and genotoxicity. Results: There was a statistically significant difference between paint workers and controls as regard total and differential blood count, serum MDA and total anti-oxidant levels. Also, statistical significant differences in caspase-3, Bcl-2 levels and 8-OHdG were observed. Conclusions: Chronic occupational exposure to paints increased the risk of hematotoxicity and genotoxicity in painters. Oxidative stress and apoptosis play a major role in such mechanism. Periodic medical examination and application of protective devices is necessary.

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Lipid Peroxidation and Antioxidative Enzyme Activity in Erythrocytes of Workers Occupationally Exposed to Aluminium

Cited by 13 publications

References 29 publications

Plasma and mitochondrial membrane perturbation induced by aluminum in human peripheral blood lymphocytes

Plasma and mitochondrial membrane perturbation induced by aluminum in human peripheral blood lymphocytes

Occupational risk assessment of oxidative stress and genotoxicity in workers exposed to paints during a working week

Assessment of Hematotoxicity and Genotoxicity among paint Workers in Assiut Governorate: a case control study

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