Lipid Oxidation Inhibitory Effects and Phenolic Composition of Aqueous Extracts from Medicinal Plants of Colombian Amazonia

Abstract

Diverse plants of ethnobotanic interest in Amazonia are commonly used in traditional medicine. We determined the antioxidant potential against lipid peroxidation, the antimicrobial activity, and the polyphenol composition of several Amazonian plants (Brownea rosademonte, Piper glandulosissimum, Piper krukoffii, Piper putumayoense, Solanum grandiflorum, and Vismia baccifera). Extracts from the plant leaf, bark, and stem were prepared as aqueous infusions, as used in folk medicine, and added to rat liver microsomes exposed to iron. The polyphenolic composition was detected by reverse-phase HPLC coupled to diode-array detector and MS/MS analysis. The antimicrobial activity was tested by the spot-on-a-lawn method against several indicator microorganisms. All the extracts inhibited lipid oxidation, except the P. glandulosissimum stem. The plant extracts exhibiting high antioxidant potential (V. baccifera and B. rosademonte) contained high levels of flavanols (particularly, catechin and epicatechin). By contrast, S. grandiflorum leaf, which exhibited very low antioxidant activity, was rich in hydroxycinnamic acids. None of the extracts showed antimicrobial activity. This study demonstrates for the first time the presence of bioactive polyphenolic compounds in several Amazonian plants, and highlights the importance of flavanols as major phenolic contributors to antioxidant activity.

Phytochemical analysis, antioxidant and antilipid peroxidation effects of a medicinal plant, Adhatoda vasica

“…At the concentration of 25 μg/ml, the reducing power efficacies of ELEAV and ascorbic acid were 0.90 and 1.21, respectively. Similarly, various plant extracts containing different phytochemicals have shown antioxidant activity through their reductive capacity in an Fe 3+ -Fe 2+ system (Lizcano et al 2012). The results obtained in this study indicate that the marked reducing power of ELEAV may be attributable to its antioxidant activity.…”

Bioactive compounds and scavenging capacity of extracts from different parts ofVismia caulifloraagainst reactive oxygen and nitrogen species

“…DOI: 10.3109/13880209.2014.974063 were found as the major compounds in leaf and stem extracts of V. baccifera (Lizcano et al, 2012). Additionally, in our study, quercetin was present in high amounts (12 mg/g extract) in flower extracts, as well as epigallocatechin methyl gallate in stem bark extracts (17 mg/g extract).…”

“…The flow rates of the sheath gas and the auxiliary gas were set to be 40 and 10, respectively (arbitrary unit as provided by the software settings) and the normalized collision energy (CID) for MS/MS experiments was 35%. The phenolic compounds were tentatively identified based on the following information: elution order, retention time of peaks, and UV-visible and mass spectra features as compared with authentic standards (data not shown) analyzed under the same conditions and data available in the literature (Bilia et al, 2000;Cuyckens & Claeys, 2004;Lizcano et al, 2012). Phenolic compounds were quantified by comparison with external standards using sixpoint analytical curves (0.6-20 mg/mL, in duplicate, r 2 !…”

Efficacy of Chromolaena odorata leaf extracts for the healing of rat excision wounds

“…The lipid peroxidation inhibition ability of the extracts was evaluated using a modified procedure described by Lizcano et al (2012). The percentage inhibition was calculated as follows:…”