Lipid Metabolism Indices and Plasma Corticosterone Concentration in Chickens Treated with Prolactin at Different Time Points

Abstract

The effect of exogenous prolactin (PRL) on the weight and lipid content of the liver and abdominal adipose tissue as well as on the concentrations of total lipids and lipoprotein fractions (portomicrons, VLDL, LDL, HDL) in plasma was investigated in chickens. PRL or its solvent were administered at different time points (0, 4, 8, and 12 hours after light onset = HALO) during 5 consecutive days. The birds were exsanguinated at the same time of the day when they received the injections, 24 hours after the last one. At the same time plasma corticosterone (CS) concentration was determined in these chickens and also during 24 hours in intact chickens matched for age. PRL affected fat accumulation in the experimental birds and the intensity of this effect depends on the time of the day at which it was administered. PRL caused rise of the weight of the fat tissue and its lipid content when it was injected at 8 or 12 HALO. This was associated with a significant increase of total plasma lipids and with an insignificant increase in the level of lipoprotein fractions excluding portomicrons. PRL increased also (at 4 HALO) or decreased (at 8 HALO) the liver weight. Handling of the birds during vehicle injections decreased the corticosterone level in the control chickens as compared with the intact ones; PRL raised the level of CS at 0 HALO significantly. The role of PRL in the intensification of fat transport is discussed.

The Role of Corticosterone in Supporting Migratory Lipogenesis in the Dark‐Eyed Junco,Junco hyemalis: A Model for Central and Peripheral Regulation

“…RU486 is also a progesterone receptor antagonist (Landys et al 2004b). Its ability to stimulate or inhibit levels of plasma prolactin (at least in mammals), another hormone linked with the development of migratory condition (Meier and Farner 1964;Meier et al 1965) and a powerful stimulator of food intake (Sotowska-Brochocka et al 1986;Koch et al 2004), could have added confounding variables to the study. Had we used RU486, we may have blocked responses to low CORT levels, but we may also have increased endogenous plasma CORT levels (Spencer et al 1998;Landys et al 2004b).…”

Effects of 3-Hydroxy-3-methylglutaryl-CoA Reductase Inhibitors on Cholesterol Metabolism in Laying Hens

“…The cholesterol synthesised by the liver is transported to the bloodstream in the form of lipoprotein [9]. There are four kinds of lipoprotein in the blood that are involved in the transport of cholesterol: portomicrons, high-density lipoprotein (HDL), low-density lipoprotein (LDL), and very low-density lipoprotein (VLDL) [10]. The VLDL synthesised by laying hens is rich in vitellogenin and has a small volume, which is not easily degraded by lipoprotein lipase [11].…”