Lipid Metabolism in Zebrafish

Ho, Shiu-Ying; Thorpe, Juanita L.; Deng, Yun; Santana, Evelyn; DeRose, Robert; Farber, Steven A.

doi:10.1016/s0091-679x(04)76006-9

Cited by 34 publications

(24 citation statements)

References 61 publications

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“…This is consistent with several reports on acyl composition of zebrafish larvae and adults [17,40,56,57]. Most of these studies were based on analyzing the acyl chain components of the different classes after hydrolyzing the phospholipid molecules.…”

Section: Discussionsupporting

confidence: 91%

“…The zebrafish has emerged as a valid model for several areas of research, including human disease [37,38], inflammation [19,21], neutrophil biology [19,39] and lipid metabolism [40]. Zebrafish has been shown to synthesize eicosanoids from exogenous precursors such as AA or PGH 2 [41,42], but endogenous eicosanoids have also been found in homogenates from these animals, including PGE 2 ,, LTB 4 , LXA 4 , 5-HETE and 12-HETE [18,43,44].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Lysophospholipid acyltransferases and eicosanoid biosynthesis in zebrafish myeloid cells

Zarini

Hankin

Murphy

et al. 2014

Prostaglandins & Other Lipid Mediators

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Eicosanoids derived from the enzymatic oxidation of arachidonic acid play important roles in a large number of physiological and pathological processes in humans. Many animal and cellular models have been used to investigate the intricate mechanisms regulating their biosynthesis and actions. Zebrafish is a widely used model to study the embryonic development of vertebrates. It expresses homologs of the key enzymes involved in eicosanoid production, and eicosanoids have been detected in extracts from adult or embryonic fish. In this study we prepared cell suspensions from kidney marrow, the main hematopoietic organ in fish. Upon stimulation with calcium ionophore, these cells produced eicosanoids including PGE2, LTB4, 5-HETE and, most abundantly, 12-HETE. They also produced small amounts of LTB5 derived from eicosapentaenoic acid. These eicosanoids were also produced in kidney marrow cells stimulated with ATP, and this production was greatly enhanced by preincubation with thimerosal, an inhibitor of arachidonate reacylation into phospholipids. Microsomes from these cells exhibited acyltransferase activities consistent with expression of MBOAT5/LPCAT3 and MBOAT7/LPIAT1, the main arachidonoyl-CoA:lysophospholipid acyltransferases. In summary, this work introduces a new cellular model to study the regulation of eicosanoid production through a phospholipid deacylation-reacylation cycle from a well-established, versatile vertebrate model species.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Lysophospholipid acyltransferases and eicosanoid biosynthesis in zebrafish myeloid cells

Zarini

Hankin

Murphy

et al. 2014

Prostaglandins & Other Lipid Mediators

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“…As the human population is exposed to several chemicals daily, many of which are bioaccumulative, the highly plausible effects these environmental pollutants have on lipid metabolism-associated diseases cannot be ignored. Zebrafish offers advantages in the study of lipid metabolism (Ho et al, 2004) as many metabolic processes, including energy homeostasis, are conserved in vertebrates (Sheridan, 1988;Pack et al, 1996;Wallace and Pack, 2003;Kadereit et al, 2008;Oka et al, 2010). Zebrafish are poikilothermic and metabolize lipids instead of carbohydrates as an energy source and, therefore, are hyperlipidemic and hypercholesterolemic when compared with mammals (Henderson and Tocher, 1987).…”

Section: Toxic Effects On Lipid Metabolismmentioning

confidence: 99%

Developmental toxicity screening in zebrafish

McCollum

Ducharme

Bondesson

et al. 2011

Birth Defects Research Part C: Embryo Today: Reviews

133

101

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Given the ever-increasing toxic exposure ubiquitously present in our environment as well as emerging evidence that these exposures are hazardous to human health, the current rodent-based regulations are proving inadequate. In the process of overhauling risk assessment methodology, a nonrodent test organism, the zebrafish, is emerging as tractable for medium- and high-throughput assessments, which may help to accelerate the restructuring of standards. Zebrafish have high developmental similarity to mammals in most aspects of embryo development, including early embryonic processes, and on cardiovascular, somite, muscular, skeletal, and neuronal systems. Here, we briefly describe the development of these systems and then chronicle the toxic impacts assessed following chemical exposure. We also compare the available data in zebrafish toxicity assays with two databases containing mammalian toxicity data. Finally, we identify gaps in our collective knowledge that are ripe for future studies.

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“…The effect of radiation on gastrointestinal function and lumen formation can be further monitored by using the fluorescent reporter PED6 (Ho et al, 2004) that is metabolized and excreted through the gastrointestinal system. Fluorescence microscopy enables a view of the gastrointestinal lumen filled with fluorescent PED6 and unquenched by the activity of phospholipase A2 present in the mucosal lining of the gastrointestinal tract (Fig.…”

Section: Radiation-associated Gastrointestinal Toxicitymentioning

confidence: 99%