Lipid metabolism in insect disease vectors

Gondim, Kátia C.; Atella, Geórgia C.; Pontes, Emerson Guedes; Majerowicz, David

doi:10.1016/j.ibmb.2018.08.005

Cited by 75 publications

(55 citation statements)

References 213 publications

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“…This reality promotes a fine balance between lipid mobilization for egg growth and lipid storage to survive starvation. Here, we demonstrate that there are different types and subtypes of enzymes involved in lipid metabolism, as reported by Gondim et al [67], and each one seems to have a particular role according to the specific tissue and physiological condition. TAG can be synthesized by 4 different pathways: a) the monoacylglycerol (MG)-pathway; b) the glycerol-3 phosphate (G3P) pathway; (c) degradation of phospholipids; or (d) deacylation of triglyceride catalyzed by lipases [9].…”

Section: Lipid Analysissupporting

confidence: 84%

“…TAG can be synthesized by 4 different pathways: a) the monoacylglycerol (MG)-pathway; b) the glycerol-3 phosphate (G3P) pathway; (c) degradation of phospholipids; or (d) deacylation of triglyceride catalyzed by lipases [9]. In R. prolixus, only the G3P pathway has been reported [67]. This pathway starts with acylation of G3P, catalyzed by G3P acyl transferases (GPAT).…”

Section: Lipid Analysismentioning

confidence: 99%

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What happens after a blood meal? A transcriptome response from the main tissues involved in egg production inRhodnius prolixus, an insect vector of Chagas disease

Leyria

Orchard

Lange

2020

Preprint

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The blood-sucking hemipteran Rhodnius prolixus is a vector of Chagas disease, one of the most neglected tropical diseases affecting several million people, mostly in Latin America.The blood meal is an event with a high epidemiological impact since in adult mated females it initiates the production of hundreds of eggs. By means of RNA-Sequencing (RNA-Seq) we have examined how a blood meal influences mRNA expression in the central nervous system (CNS), fat body and ovaries in order to promote egg production, focusing on tissue-specific responses under controlled nutritional conditions. We illustrate the cross talk between reproduction and a) lipids, proteins and trehalose metabolism, b) neuropeptide and neurohormonal signaling, and c) the immune system. Overall, our molecular evaluation confirms and supports previous studies and provides an invaluable molecular resource for future investigations on different tissues involved in successful reproductive events. Analyses like this can be used to increase the chances of developing novel strategies of vector population control by translational research, with less impact on the environment and more specificity for a particular organism.

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Section: Lipid Analysissupporting

confidence: 84%

Section: Lipid Analysismentioning

confidence: 99%

What happens after a blood meal? A transcriptome response from the main tissues involved in egg production inRhodnius prolixus, an insect vector of Chagas disease

Leyria

Orchard

Lange

2020

Preprint

View full text Add to dashboard Cite

show abstract

“…2 ). Most of the putatively identified virus-modulated lipids are glycerophospholipids (Supplementary Data 1 ) 33 – 35 . Consistent with the study by Perera et al 29 of DENV2 infection in mosquito cells, many of the altered glycerophospholipids possessed fatty acids with greater levels unsaturation.…”

Section: Resultsmentioning

confidence: 99%

Dengue virus dominates lipid metabolism modulations in Wolbachia-coinfected Aedes aegypti

Koh

Islam

et al. 2020

Commun Biol

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Competition between viruses and Wolbachia for host lipids is a proposed mechanism of Wolbachia-mediated virus blocking in insects. Yet, the metabolomic interaction between virus and symbiont within the mosquito has not been clearly defined. We compare the lipid profiles of Aedes aegypti mosquitoes bearing mono- or dual-infections of the Wolbachia wMel strain and dengue virus serotype 3 (DENV3). We found metabolic signatures of infection-induced intracellular events but little evidence to support direct competition between Wolbachia and virus for host lipids. Lipid profiles of dual-infected mosquitoes resemble those of DENV3 mono-infected mosquitoes, suggesting virus-driven modulation dominates over that of Wolbachia. Interestingly, knockdown of key metabolic enzymes suggests cardiolipins are host factors for DENV3 and Wolbachia replication. These findings define the Wolbachia-DENV3 metabolic interaction as indirectly antagonistic, rather than directly competitive, and reveal new research avenues with respect to mosquito × virus interactions at the molecular level.

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“…In hematophagous insects, the bloodmeal triggers the secretion of neuropeptides and hormones to the circulation that, in turn, activate lipid metabolism in peripheral tissues, including the fat body (Gondim et al 2018, Li et al 2019). This organ accumulates lipids mostly in the form of triacylglycerol, and these reserves will help the insect to withstand fasting periods (Arrese and Soulages 2010).…”

Section: Discussionmentioning

confidence: 99%

The Fat Body of the Hematophagous Insect, Panstrongylus megistus (Hemiptera: Reduviidae): Histological Features and Participation of the β-Chain of ATP Synthase in the Lipophorin-Mediated Lipid Transfer

Fruttero

Leyria

Moyetta

et al. 2019

Journal of Insect Science

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In insects, lipid transfer to the tissues is mediated by lipophorin, the major circulating lipoprotein, mainly through a nonendocytic pathway involving docking receptors. Currently, the role of such receptors in lipid metabolism remains poorly understood. In this work, we performed a histological characterization of the fat body of the Chagas’ disease vector, Panstrongylus megistus (Burmeister), subjected to different nutritional conditions. In addition, we addressed the role of the β-chain of ATP synthase (β-ATPase) in the process of lipid transfer from lipophorin to the fat body. Fifth-instar nymphs in either fasting or fed condition were employed in the assays. Histological examination revealed that the fat body was composed by diverse trophocyte phenotypes. In the fasting condition, the cells were smaller and presented a homogeneous cytoplasmic content. The fat body of fed insects increased in size mainly due to the enlargement of lipid stores. In this condition, trophocytes contained abundant lipid droplets, and the rough endoplasmic reticulum was highly developed and mitochondria appeared elongated. Immunofluorescence assays showed that the β-ATPase, a putative lipophorin receptor, was located on the surface of fat body cells colocalizing partially with lipophorin, which suggests their interaction. No changes in β-ATPase expression were found in fasting and fed insects. Blocking the lipophorin–β-ATPase interaction impaired the lipophorin-mediated lipid transfer to the fat body. The results showed that the nutritional status of the insect influenced the morphohistological features of the tissue. Besides, these findings suggest that β-ATPase functions as a lipophorin docking receptor in the fat body.

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Lipid metabolism in insect disease vectors

Cited by 75 publications

References 213 publications

What happens after a blood meal? A transcriptome response from the main tissues involved in egg production inRhodnius prolixus, an insect vector of Chagas disease

What happens after a blood meal? A transcriptome response from the main tissues involved in egg production inRhodnius prolixus, an insect vector of Chagas disease

Dengue virus dominates lipid metabolism modulations in Wolbachia-coinfected Aedes aegypti

The Fat Body of the Hematophagous Insect, Panstrongylus megistus (Hemiptera: Reduviidae): Histological Features and Participation of the β-Chain of ATP Synthase in the Lipophorin-Mediated Lipid Transfer

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