Lipid Metabolism in Ethanol-Treated Rat Pups and Adults: Effects of Folic Acid

Ojeda, María Luisa; Delgado-Villa, Ma Jesús; Llopis, R; Murillo, María Luisa Ojeda; Carreras, Olimpia

doi:10.1093/alcalc/agn044

Cited by 33 publications

(28 citation statements)

References 42 publications

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“…As GPx4 acts protecting against oxidation of the membrane phospholipids, it could play an important role during lactation (when there is an intense phase of cell formation), protecting the newly synthesized phospholipids. Taking into account that in this period of life the amount of phospholipid vs triglycerides is higher, the lipid profile is different to that found in adulthood (Ojeda et al 2008); the increase in GPx4 could be related to the absence of lipid oxidation in the groups studied. Based on this information, it can be concluded that GPx1 is very sensitive, and GPx4 is highly resistant, to dietary Se deficiency in offspring, and according to this, as GPx1 expression is fully committed, in SD pups protein oxidation occurs; however, as GPx4 expression is not completely decreased, phospholipid Selenium and development in pups oxidation does not take place.…”

Section: Selenoprotein Expressionmentioning

confidence: 58%

Role of selenium and glutathione peroxidase on development, growth, and oxidative balance in rat offspring

Nogales¹,

Ojeda²,

Fenutría³

et al. 2013

Reproduction

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Selenium (Se), an essential trace metal, is important in both growth and reproduction and is the constituent of different selenoproteins. The glutathione peroxidase (GPx) family is the most studied as it prevents oxidative stress. Liver oxidation is considered as another mechanism involved in low birth weight. Therefore, in order to ascertain whether GPx is related to the effects of Se on growth during gestation and lactation, three groups of rat pups were used: control, Se deficient (SD), and Se supplemented (SS). Morphological parameters and reproductive indices were evaluated. Hepatic Se levels were measured by graphite furnace atomic absorption while spectrophotometry was used for activity of antioxidant enzymes and oxidative stress markers in liver and western blotting for expression of hepatic GPx1 and GPx4. The SD diet increased mortality at birth; decreased viability and survival indices; and stunted growth, length, and liver development in offspring, thus decreasing hepatic Se levels, GPx, glutathione reductase, and catalase activities, while increasing superoxide dismutase activity and protein oxidation. The SS diet counteracted all the above results. GPx1 expression was heavily regulated by Se dietary intake; however, although Se dietary deficiency reduced GPx4 expression, this decrease was not as pronounced. Therefore, it can be concluded that Se dietary intake is intimately related to growth, length, and directly regulating GPx activity primarily via GPx1 and secondly to GPx4, thus affecting liver oxidation and development. These results suggest that if risk of uterine growth retardation is suspected, or if a neonate with low birth weight presents with signs of liver oxidation, it may be beneficial to know about Se status.

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Section: Selenoprotein Expressionmentioning

confidence: 58%

Role of selenium and glutathione peroxidase on development, growth, and oxidative balance in rat offspring

Nogales¹,

Ojeda²,

Fenutría³

et al. 2013

Reproduction

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“…We found the highest serum GPx activity in AFSO. It seems that folic acid acts synergetically with selenium to increase serum GPx activity, especially during alcohol consumption (where serum basal folic acid concentration decreases) (Ojeda et al, 2008). Possibly, the greatest increase in serum Se was provoked by Se-only supplementation to ethanolexposed rats' pups, something reflected in an increase in Selenoprotein P, the main serum Se transporters (Hollenbach et al, 2008), nor in GPx.…”

Section: Discussionmentioning

confidence: 99%

“…Male (n 5 6) and female (n 5 6) rats were mated to obtain the 1st-generation offspring for each group. After reproduction, pregnant rats were housed individually in plastic cages, and continued their chronic ethanol treatment until the end of the lactation period, so alcohol was supplied to mothers for 13 weeks (induction, reproduction, gestation, and lactation periods) (Ojeda et al, 2008). The day of parturition was designated as day 1 of lactation, the number of offspring being reduced to 10 per mother at parturition, and day 21 designated as the final day of the lactation period.…”

Section: Materials and Methods Animalsmentioning

confidence: 99%

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Dietary selenium plus folic acid as an antioxidant therapy for ethanol‐exposed pups

Ojeda

Nogales

Jotty

et al. 2009

Birth Defects Research Pt B

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“…Furthermore, the highest affinity found in the AFS offspring could be explained by an extra protective effect of folic acid on lipid membranes. Ojeda et al (2008) [20] demonstrated different lipid alterations in adults and in pups whose dams consumed ethanol and that folic acid contributes to alleviation of the adverse effects in these cases. Se-Met absorption was lower in all the control groups studied compared with their alcohol counterparts, probably because paracellular absorption is reduced, since Se-Met, in terms of absorption, acts as an amino acid.…”

Section: Duodenal Absorption Of Selenomethioninementioning

confidence: 99%

Effects of Antioxidant Supplementation on Duodenal Se-Met Absorption in Ethanol-exposed Rat Offspring In Vivo

Nogales

Ojeda

Delgado

et al. 2011

Journal of Reproduction and Development

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Abstract. The nutritional deficiencies provoked by ethanol consumption, during gestation or lactation, can contribute to multiple birth defects in offspring. In order to improve our knowledge about selenium (Se) distribution in pups exposed to ethanol, the present study evaluated the effect of this drug on intestinal development and determined its action on duodenal absorption of selenomethionine (Se-Met). To determinate if supplementation could improve Se absorption and its serum values, we used two antioxidant supplemented regimens on dams, with selenium alone or selenium plus folic acid, and obtained six groups of pups: C (control), A (alcohol), CS (control + Se), AS (alcohol + Se), CFS (control + Se + folic acid) and AFS (alcohol + Se + folic acid). Duodenal Se-Met transport was performed using an in vivo perfusion method. Se levels were measured by graphite furnace atomic absorption spectrometry. The supplemented diets utilized had a positive influence on body growth, duodenal perimeter and Se content in ethanol-exposed pups. Ethanol exposure increased Se-Met duodenal absorption in all pups, supplemented or not, presenting the highest values of maximal velocity (V max ) compared with their control counterparts. The affinity constant (K m ) increased according to rank: A>AS>AFS groups. These results suggest that although antioxidant supplementation does not restore Se-Met absorption to normal values, it enhances the affinity of the transporters for the substrate and improves the damage caused by ethanol in the duodenal mucosa.Key words: Ethanol, Kinetic parameters, Offspring development, Selenomethionine absorption (J. Reprod. Dev. 57: [708][709][710][711][712][713][714] 2011) T he primary function of the intestinal epithelium is to absorb the small molecules produced from food digestion [1]. However, specific substances such as alcohol could alter this intestinal absorption [2,3], producing malnutrition. Numerous studies have demonstrated that chronic ethanol consumption induces morphological and biochemical alterations in the intestinal mucosal cells [4] and that some of these changes are caused by a lipid peroxidation of duodenal membrane lipids [5]. These effects of ethanol exposure are more acute during pregnancy and lactation and can contribute to a delay in offspring growth and multiple birth defects, including fetal alcohol syndrome [6]. In this context, it is especially relevant that ethanol deregulates the homeostasis of different antioxidants such as folic acid, whose deficiency is the most common sign of malnutrition in chronic alcoholism [7]. This deficiency is associated with several alterations such as Down's syndrome and defects in neural tube closure in developing embryos [1]. According to what we have previously reported [8], the effects of ethanol exposure during gestation and lactation on folic acid intestinal absorption showed that intestinal folate absorption appears to be upregulated in suckling rats. Our research group has also found, in pups exposed to ethanol and supplemented wit...

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Lipid Metabolism in Ethanol-Treated Rat Pups and Adults: Effects of Folic Acid

Cited by 33 publications

References 42 publications

Role of selenium and glutathione peroxidase on development, growth, and oxidative balance in rat offspring

Role of selenium and glutathione peroxidase on development, growth, and oxidative balance in rat offspring

Dietary selenium plus folic acid as an antioxidant therapy for ethanol‐exposed pups

Effects of Antioxidant Supplementation on Duodenal Se-Met Absorption in Ethanol-exposed Rat Offspring In Vivo

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