Lipid metabolism and resistin gene expression in insulin-resistant Fischer 344 rats

Abstract

. Lipid metabolism and resistin gene expression in insulin-resistant Fischer 344 rats. Am J Physiol Endocrinol Metab 282: E626-E633, 2002; 10.1152/ajpendo. 00346.2001The interrelationship between insulin and leptin resistance in young Fischer 344 (F344) rats was studied. Young F344 and Sprague-Dawley (SD) rats were fed regular chow. F344 animals had two-to threefold higher insulin and triglyceride concentrations and increased stores of triglycerides within liver and muscle. F344 animals gained more body fat. Both acyl-CoA oxidase (ACO) and carnitine palmitoyltransferase I gene expression were 20-50% less in F344 animals than in age-matched SD animals. Peroxisome proliferator-activated receptor-␣ gene expression was reduced in 70-day-old F344 animals. Finally, resistin gene expression was similar in 70-day-old SD and F344 animals. Resistin gene expression increased fivefold in F344 animals and twofold in SD animals from 70 to 130 days, without a change in insulin sensitivity. We conclude that young F344 animals have both insulin and leptin resistance, which may lead to diminished fatty oxidation and accumulation of triglycerides in insulin-sensitive target tissues. We did not detect a role for resistin in the etiology of insulin resistance in F344 animals.Fischer 344 rats; acyl-coenzyme A oxidase; carnitine palmitoyltransferase I; peroxisome proliferator-activated receptor-␣

Serum concentrations of resistin-like molecules β and γ are elevated in high-fat-fed and obese db/db mice, with increased production in the intestinal tract and bone marrow

“…Further analysis, considering the crystal structure data of resistin and RELMβ, is necessary to clarify the mechanism of homo/heterodimer formation of resistin family proteins [20]. This is the first study to investigate the serum concentrations and tissue contents of RELMβ and RELMγ in insulin-resistant animals, while resistin expression has been analysed in several models of obesity and diabetes [8,11,[25][26][27][28][29][30]. In high-fat-fed mice and db/db mice, serum levels of RELMβ and RELMγ were apparently increased.…”

Effect of age and moderate food restriction on insulin sensitivity in Wistar rats: role of adiposity

“…The ratio of DGlucose area to DInsulin area was used as an index of whole body insulin sensitivity (Levy et al 2002).…”

Adipotsitokiny v nauchnoy i klinicheskoy praktike

“…У грызунов резистин высоко и специфично экс-прессируется белой жировой тканью [30], в то время как у человека экспрессия резистина в адипоцитах происходит на значительно более низком уровне, и ос-новным ресурсом резистина в жировой ткани явля-ются макрофаги [14]. Физиологическая роль резистина изучена недостаточно, и данные разных исследований зачастую противоречивы, что может быть объяснено методологическими и популяционными различиями.…”