Lipid mediators of inflammation as novel plasma biomarkers to identify patients with bacteremia

To, Kelvin Kai-Wang; Lee, Kim-Chung; Wong, S.P.; Lo, Kin‐Yee; Lui, Yanni Y N; Jahan, Akhee Sabiha; Wu, Andi; Ke, Yihong; Law, Chun Yiu; Sze, Kong-Hung; Lau, Susanna K.P.; Woo, Patrick C. Y.; Lam, Chi Fung; Yuen, Kwok‐Yung

doi:10.1016/j.jinf.2015.02.011

Cited by 32 publications

(28 citation statements)

References 36 publications

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“…PalC, an acylcarnitine involved in the transport of fatty acids across the inner mitochondrial membrane for β-oxidation, was identified as one of these molecules. PalC was of particular interest as this increase in infected MLNs is corroborated by previous studies that found increased levels of PalC in lymph in a rat sepsis model, while higher levels of acyl carnitines are present in the plasma of patients with bacteraemia 29, 42 . However the results obtained here suggest the increased abundance and effects of PalC during S .…”

Section: Discussionsupporting

confidence: 60%

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Mass spectrometry imaging identifies palmitoylcarnitine as an immunological mediator during Salmonella Typhimurium infection

Hulme

Meikle

Wessel

et al. 2017

Sci Rep

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Salmonella Typhimurium causes a self-limiting gastroenteritis that may lead to systemic disease. Bacteria invade the small intestine, crossing the intestinal epithelium from where they are transported to the mesenteric lymph nodes (MLNs) within migrating immune cells. MLNs are an important site at which the innate and adaptive immune responses converge but their architecture and function is severely disrupted during S. Typhimurium infection. To further understand host-pathogen interactions at this site, we used mass spectrometry imaging (MSI) to analyse MLN tissue from a murine model of S. Typhimurium infection. A molecule, identified as palmitoylcarnitine (PalC), was of particular interest due to its high abundance at loci of S. Typhimurium infection and MLN disruption. High levels of PalC localised to sites within the MLNs where B and T cells were absent and where the perimeter of CD169+ sub capsular sinus macrophages was disrupted. MLN cells cultured ex vivo and treated with PalC had reduced CD4+CD25+ T cells and an increased number of B220+CD19+ B cells. The reduction in CD4+CD25+ T cells was likely due to apoptosis driven by increased caspase-3/7 activity. These data indicate that PalC significantly alters the host response in the MLNs, acting as a decisive factor in infection outcome.

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Section: Discussionsupporting

confidence: 60%

“…PalC had no known direct influence on the immune response in vivo , despite research confirming its presence in bacterial and inflammatory diseases 29, 42, 43 . Here, for the first time through the use of MSI, we have spatially and temporally linked S .…”

Section: Discussionmentioning

confidence: 95%

Mass spectrometry imaging identifies palmitoylcarnitine as an immunological mediator during Salmonella Typhimurium infection

Hulme

Meikle

Wessel

et al. 2017

Sci Rep

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“…(8) and To et al . (14) report relatively high ROC AUCs between ≈0.8 and > ≈0.9 for certain serum lipid metabolites encourages further research in the search for the most definitive and unique lipid biomarkers for bacteremia and sepsis diagnostics.…”

mentioning

confidence: 86%

“…Other recent studies (13,14) report on single lipid mediators of inflammation for bacteremia based on the chemical classes of sphingolipids, acylcarnitines, glycero-phosphocholines and fatty acid esters; a number of compounds from these classes were able to distinguish bacteremia patients from non-bacteremia controls yielding ROC-estimated AUCs between ≈0.82 (for fatty acid esters) to ≈0.96 (for acylcarnitines). ROC curve sensitivities ranged from ≈76% to ≈84% and specificities from ≈75% to ≈98%, again implying appreciable estimated false positive and estimated false negative rates by some of these lipid biomarkers.…”

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confidence: 95%

Metabolomics and Receiver Operating Characteristic Analysis: A Promising Approach for Sepsis Diagnosis*

Bünger

Mallet

2016

Critical Care Medicine

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“…Taken together with the fact that sepsis, as mentioned above, is a global health concern, this underlines the significance of metabolomics for public health. More specifically, by complementing other “-omics” technologies, employment of metabolomics has the potential to radically transform the research and clinical landscape in sepsis by enabling effective risk stratification and diagnosis [100], prognosis [101], and, with pharmacometabolomics, even targeted therapeutic approaches [102]. …”

Section: Impact On Public Healthmentioning

confidence: 99%

Metabolomics in Sepsis and Its Impact on Public Health

Evangelatos

Bauer

Reumann

et al. 2017

Public Health Genomics

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Sepsis, with its often devastating consequences for patients and their families, remains a major public health concern that poses an increasing financial burden. Early resuscitation together with the elucidation of the biological pathways and pathophysiological mechanisms with the use of “-omics” technologies have started changing the clinical and research landscape in sepsis. Metabolomics (i.e., the study of the metabolome), an “-omics” technology further down in the “-omics” cascade between the genome and the phenome, could be particularly fruitful in sepsis research with the potential to alter the clinical practice. Apart from its benefit for the individual patient, metabolomics has an impact on public health that extends beyond its applications in medicine. In this review, we present recent developments in metabolomics research in sepsis, with a focus on pneumonia, and we discuss the impact of metabolomics on public health, with a focus on free/libre open source software.

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Lipid mediators of inflammation as novel plasma biomarkers to identify patients with bacteremia

Cited by 32 publications

References 36 publications

Mass spectrometry imaging identifies palmitoylcarnitine as an immunological mediator during Salmonella Typhimurium infection

Mass spectrometry imaging identifies palmitoylcarnitine as an immunological mediator during Salmonella Typhimurium infection

Metabolomics and Receiver Operating Characteristic Analysis: A Promising Approach for Sepsis Diagnosis*

Metabolomics in Sepsis and Its Impact on Public Health

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