Lipid Mediators and Lung Function

Weinberger, Barry; Hirsch, Daniel; Yin, Kingsley; Spur, Bernd W.

doi:10.1016/b978-0-12-404577-4.00021-7

“…These compounds are indeed considered to be the best biomarkers of oxidative stress status and lipid peroxidation (Czerska et al, 2016). Isoprostanes are prostaglandin (PG)-like substances produced in vivo independently of cyclooxygenase enzymes, primarily by free radical-induced peroxidation of arachidonic acid (Montuschi et al, 2004) as well as other PUFA such as docosahexaenoic acid and eicosapentaenoic acid (Weinberger et al, 2015). Concentrations of IsoP produced by BAEC after exposure to PMG natural extract and AAPH are reported in Table 1.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of natural plant extracts as antioxidants in a bovine in vitro model of oxidative stress

Ciampi

¹

,

Sordillo

²

,

Gandy

³

et al. 2020

Journal of Dairy Science

View full text Add to dashboard Cite

Oxidative stress contributes to many inflammatorybased diseases of dairy cattle especially during periods of increased metabolic activity such as around calving. Endothelial cells play a key role in maintaining normal inflammatory responses, but they are especially susceptible to macromolecule damage during times of oxidative stress. Therefore, bovine aortic endothelial cells (BAEC) were used to study the effect of natural tanninbased extracts on oxidative stress that may improve health and well-being of cattle. Tannins are secondary metabolites in plants with potent antioxidant activity that have been used as natural feed additives for foodproducing animals. However, there is little information on how tannin-rich plant extracts may affect oxidative stress in dairy cattle. The objective of this study was to evaluate the antioxidant effect of pomegranate (Punica granatum; PMG), tara (Caesalpinia spinosa; TA), chestnut (Castanea sativa; CH), and gambier (Uncaria gambir; GM) natural extracts using an in vitro BAEC model of oxidative stress. Natural extracts were tested at a concentration of 80 μg/mL. Viability, apoptosis, intracellular reactive oxygen species, and isoprostanes were determined on cultured BAEC treated with different plant natural extracts. No changes in cell viability was detected following PMG and GM treatments. In contrast, there was a 30% reduction of BAEC viability following treatment with CH or TA extracts. Intracellular reactive oxygen species production was significantly less abundant in cells treated with natural extracts than with the lipopolysaccharide control. Moreover, antioxidant activity varied according to the tested extract, showing a reduction of 63, 45, 51, and 27% in PMG, GM, CH, and TA, respectively. The formation of isoprostanes as a consequence of lipid peroxidation after induction of oxidative stress also were significantly decreased in PMG-treated cells when compared with the untreated cells. Theses findings suggest that PMG extract has the potential to mitigate oxidative stress without detrimental effects on cell viability. Further in vitro and in vivo research is warranted to explore the antioxidant potential of PMG extract as a dietary supplement to control oxidative stress in dairy cattle.

show abstract

“…Este ácido, por sua vez, produz eicosanoides pela via da cicloxigenase. Os corticoides atuam no bloqueio da fosfolipase A 2 e impedem a formação do ácido araquidônico (Figura 3) (Leslie, 2015;Weinberger et al, 2015). Fonte: adaptada de Ramamoorthy & Cidlowski (2016).…”

Section: Fosfolipasesunclassified

Plantas Medicinais Como Terapia Alternativa No Envenenamento Por Serpentes

Chaves¹,

Santos²,

Mendes³

et al. 2021

Medicina: Ciências Da Saúde E Pesquisa Interdisciplinar

0

View full text Add to dashboard Cite

show abstract

“…Este ácido, por sua vez, produz eicosanoides pela via da cicloxigenase. Os corticoides atuam no bloqueio da fosfolipase A 2 e impedem a formação do ácido araquidônico (Figura 3) (Leslie, 2015;Weinberger et al, 2015). Fonte: adaptada de Ramamoorthy & Cidlowski (2016).…”

Section: Fosfolipasesunclassified