Lipid-lowering effects of Coffea arabica pulp aqueous extract in Caco-2 cells and hypercholesterolemic rats

Ontawong, Atcharaporn; Duangjai, Acharaporn; Muanprasat, Chatchai; Pasachan, Tipthida; Pongchaidecha, Anchalee; Amornlerdpison, Doungporn; Srimaroeng, Chutima

doi:10.1016/j.phymed.2018.06.021

Cited by 40 publications

(31 citation statements)

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“…Several atherogenic factors have been found to increase the expression of intestinal NPC1L1 and then elevate the levels of blood cholesterols (24,38). In addition, level reduction of NPC1L1 is reported to be an effective way to inhibit cholesterol absorption, thereby ameliorating dyslipidemia and suppressing atherosclerosis progression (39,40). There is evidence that cholesterol secretion from enterocytes to the gut lumen is regulated by apical transporters ABCG5 and ABCG8 (16,24).…”

Section: Discussionmentioning

confidence: 99%

The atheroprotective roles of heart-protecting musk pills against atherosclerosis development in apolipoprotein E-deficient mice

et al. 2019

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Background: Heart-protecting musk pill (HMP), derived from Chinese herbal medicines, has been found to possess protective roles against atherosclerosis-related cardiovascular diseases (CVDs), however, the antiatherosclerotic mechanisms of HMP are still unclear. Here, we investigated the effects of HMP on alleviating atherosclerotic lesion severity in mice and explored the molecular mechanisms.Methods: Apolipoprotein E-deficient mice were fed western-type diet supplemented with HMP (25 mg/kg/day) or normal saline gavage for 20 weeks. Then histopathological staining was performed to assess the atheromatous plaque burden. Biochemical kits were used to detect levels of lipid profiles. Moreover, effector factors associated with lipid metabolism in liver and intestinal tissues were investigated by western blot and real-time PCR assays. Levels of signal molecules participating in the mitochondrial-mediated apoptosis pathway were detected by Western blot.Results: We found that HMP notably reduced atherosclerotic lesion size (P<0.05) and improved plaque stability (P<0.05). HMP treatment decreased circulating TC (P<0.01), LDL-C (P<0.01) and TG (P<0.05) levels and increased HDL-C (P<0.05) content. HMP was found to suppress SREBP2, HMGCR and PCSK9 expressions (P<0.05), yet promote LDLR expression (P<0.05) in hepatocytes. Moreover, HMP was discovered to activate PPARα/CPT-1A cascade (P<0.05) and inhibit contents of SREBP1c and the lipogenic genes FAS and ACCα (P<0.05). The LBK1/AMPK cascade was also activated after HMP administration (P<0.05). Additionally, HMP was found to facilitate transintestinal cholesterol excretion by increasing ABCG5 and ABCG8 levels and reducing NPC1L1 content (P<0.05). In terms of vasoprotective activities, we observed that HMP decreased cleaved caspase-3 content (P<0.05) in the vascular intima, which might be due to inhibition of mitochondrial-related signaling pathway.Conclusions: Altogether, our study indicates that HMP plays anti-atherosclerotic roles via regulating lipid metabolism and improving vascular intimal injury.

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Section: Discussionmentioning

confidence: 99%

The atheroprotective roles of heart-protecting musk pills against atherosclerosis development in apolipoprotein E-deficient mice

et al. 2019

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“…The authors in [66] conducted a hypoglycemic effect study on small coffee and its leaf ethanol extract, and found that coffee extract can significantly reduce the blood sugar content of mice. Caffeol has potential anti-diabetic effects which can increase glucose-stimulated insulin secretion and increase the uptake of glucose by human skeletal muscle cells [67].…”

Section: Lowering Blood Sugarmentioning

confidence: 99%

Relationship between the Chemical Composition and the Biological Functions of Coffee

Saud

Salamatullah

2021

Molecules

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Coffee is a Rubiaceae coffee plant ranked as the first of the three most important beverages in the world, with effects including lowering blood sugar, protecting the liver, and protecting the nerves. Coffee contains many chemical components, including alkaloids, phenolic acids, flavonoids, terpenoids, and so on. Chemical components in coffee are the basis of its biological function and taste. The chemical components are the basis of biological activities and form the characteristic aroma of coffee. The main chemical components and biological activities of coffee have been extensively studied, which would provide a relevant basis and theoretical support for the further development of the coffee industry.

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“…NU003806. CPE was extracted according to our recent report [19]. Addi-tionally, phenolic contents, including chlorogenic acid, caffeine, catechin, and epicatechin, were also recently identified from our report [19].…”

Section: Coffea Arabica Pulp Aqueous Extract Preparation and Total Phenolic Content Measurementmentioning

confidence: 99%

“…CPE was extracted according to our recent report [19]. Addi-tionally, phenolic contents, including chlorogenic acid, caffeine, catechin, and epicatechin, were also recently identified from our report [19]. Briefly, dried CP was ground, blended, and infused with boiled water at 100 • C for 10 min.…”

Section: Coffea Arabica Pulp Aqueous Extract Preparation and Total Phenolic Content Measurementmentioning

confidence: 99%

“…Subsequently, the animals were randomly divided into 6 groups: normal diet (ND), ND supplemented with CPE at 1000 mg/kg BW (ND + CPE), T2D (DM), DM supplemented with CPE at 1000 mg/kg BW (DM + CPE), DM treated with metformin at 30 mg/kg BW (DM + Met), and DM receiving a combination of CPE at 1000 mg/kg BW and metformin at 30 mg/kg BW (DM + CPE + Met). The selection of this single, high dose was due to our recent study demonstrating that 1000 mg/kg BW of CPE contains approximately 12 mg/kg BW CGA [19]. Additionally, CGA, at a dose of 10 mg/kg BW, improves renal function and oxidative stress in DN rats [47], while metformin, at a dose of 30 mg/kg BW, exhibits antidiabetic effects in STZ-induced diabetic rats [48].…”

Section: Animals and Induction Of Experimental Type 2 Diabetic Ratsmentioning

confidence: 99%

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Antidiabetic and Renoprotective Effects of Coffea arabica Pulp Aqueous Extract through Preserving Organic Cation Transport System Mediated Oxidative Stress Pathway in Experimental Type 2 Diabetic Rats

et al. 2021

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Coffea arabica pulp (CP) is a by-product of coffee processing. CP contains polyphenols that have exhibited beneficial effects, including antioxidant and lipid-lowering effects, as well as enhanced insulin sensitivity, in in vitro and in vivo models. How polyphenols, as found in CP aqueous extract (CPE), affect type 2 diabetes (T2D) has not been investigated. Thus, the present study examined the potential antidiabetic, antioxidant, and renoprotective effects of CPE-rich polyphenols, using an experimental model of T2D in rats induced by a high-fat diet and a single low dose of streptozotocin. The T2D rats received either 1000 mg/kg body weight (BW) of CPE, 30 mg/kg BW of metformin (Met), or a combination treatment (CPE + Met) for 3 months. Plasma parameters, kidney morphology and function, and renal organic transport were determined. Significant hyperglycemia, hypertriglyceridemia, insulin resistance, increased renal lipid content and lipid peroxidation, and morphological kidney changes related to T2D were restored by both CPE and CPE + Met treatments. Additionally, the renal uptake of organic cation, 3H-1-methyl-4-phenylpyridinium (MPP+), was reduced in T2D, while transport was restored by CPE and CPE + Met, through an up-regulation of antioxidant genes and protein kinase Cα deactivation. Thus, CPE has antidiabetic and antioxidant effects that potentially ameliorate kidney function in T2D by preserving renal organic cation transport through an oxidative stress pathway.

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Lipid-lowering effects of Coffea arabica pulp aqueous extract in Caco-2 cells and hypercholesterolemic rats

Cited by 40 publications

References 31 publications

The atheroprotective roles of heart-protecting musk pills against atherosclerosis development in apolipoprotein E-deficient mice

The atheroprotective roles of heart-protecting musk pills against atherosclerosis development in apolipoprotein E-deficient mice

Relationship between the Chemical Composition and the Biological Functions of Coffee

Antidiabetic and Renoprotective Effects of Coffea arabica Pulp Aqueous Extract through Preserving Organic Cation Transport System Mediated Oxidative Stress Pathway in Experimental Type 2 Diabetic Rats

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