Lipid headgroup and side chain architecture determine manganese-induced dose dependent membrane rigidification and liposome size increase

Sule, Kevin; Prenner, Elmar J.

doi:10.1007/s00249-022-01589-x

Cited by 6 publications

(11 citation statements)

References 101 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Structural differences between PA and PS will determine the ion-lipid interactions. The phosphate headgroup of PA is small and readily accessible to ions and, under our experimental conditions, carries a net charge of −1.2 [ 29 , 30 ]. In contrast, PS caries a zwitterionic serine head group linked with a phosphate group to the glycerol backbone and has an overall negative charge of −1 [ 31 ].…”

Section: Resultsmentioning

confidence: 99%

“…We have extensively utilized biomimetic model and complex biological extracts to investigate the membrane interaction of divalent cations such as Cd, Hg, Co, Ni and Mn [ 29 , 44 , 45 , 46 ] as a function of lipid composition. Important factors such as metal speciation as well as unspecific binding to buffers are often not considered but significantly affect these interactions (for a review, see [ 47 ]).…”

Section: Discussionmentioning

confidence: 99%

“…Both PA and PS were attractive targets for Mn, which exuded a relatively stronger impact on membrane fluidity and liposome size, compared to Ca and Mg. Mn binding to anionic phospholipid membranes has been reported, resulting in increased membrane rigidity, permeability and aggregation [ 17 , 29 , 51 ]. Moreover, Mn interaction with POPA and DMPA revealed strong membrane rigidification, liposome size increases and shifts in T m [ 29 ].…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Lipid Structure Determines the Differential Impact of Single Metal Additions and Binary Mixtures of Manganese, Calcium and Magnesium on Membrane Fluidity and Liposome Size

Sule

Anikovskiy

Prenner

2023

IJMS

Self Cite

View full text Add to dashboard Cite

Unilamellar vesicles of the biologically relevant lipids phosphatidic acid (PA) and phosphatidylserine (PS) with fully saturated (DM-) or partly unsaturated (PO-) acyl side chains were exposed to Ca, Mn and Mg in single metal additions; in equimolar mixtures or by sequential additions of one metal at a time. Laurdan generalized polarization measured the membrane fluidity, while dynamic light scattering reported liposome size changes complemented by zeta potential. All metals induced membrane rigidity and increased liposome sizes across all systems. Mn had the strongest effect overall, but Mg was comparable for DMPS. Lipid side chain architecture was important as GP values for binary mixtures were higher than expected from the sum of values for single additions added to POPS but smaller for DMPS. Sequential additions were predominantly different for Ca:Mg mixtures. Mn induced the strongest increase of liposome size in saturated lipids whereas Ca effects dominated unsaturated matrices. Binary additions induced larger sizes than the sum of single additions for POPS, but much lower changes in DMPA. The order of addition was relevant for PS systems. Thus, lipid structure determines metal effects, but their impact is modulated by other ions. Thus, metal effects may differ with the local lipid architecture and metal concentrations within cells.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Lipid Structure Determines the Differential Impact of Single Metal Additions and Binary Mixtures of Manganese, Calcium and Magnesium on Membrane Fluidity and Liposome Size

Sule

Anikovskiy

Prenner

2023

IJMS

Self Cite

View full text Add to dashboard Cite

show abstract

“…The starting GP value of 0.13 also reflects a fluid membrane ( Figure 2 ). PCs contain the largest head group of the glycerophospholipid classes, which—combined with their zwitterionic nature—limit the interactions of divalent ions (e.g., Cd [ 25 ], Mn [ 27 ] Co, Ni [ 28 ]). The addition of metals to the liposomes limits their interactions with the outer leaflet during the time course of these experiments.…”

Section: Resultsmentioning

confidence: 99%

Gadolinium Effects on Liposome Fluidity and Size Depend on the Headgroup and Side Chain Structure of Key Mammalian Brain Lipids

Farzi,

Issler,

Unruh

et al. 2023

Molecules

Self Cite

View full text Add to dashboard Cite

The lanthanide metal gadolinium has been used in the healthcare industry as a paramagnetic contrast agent for years. Gadolinium deposition in brain tissue and kidneys has been reported following gadolinium-based contrast agent administration to patients undergoing MRI. This study demonstrates the detrimental effects of gadolinium exposure at the level of the cell membrane. Biophysical analysis using fluorescence spectroscopy and dynamic light scattering illustrates differential interactions of gadolinium ions with key classes of brain membrane lipids, including phosphatidylcholines and sphingomyelins, as well as brain polar extracts and biomimetic brain model membranes. Electrostatic attraction to negatively charged lipids like phosphatidylserine facilitates metal complexation but zwitterionic phosphatidylcholine and sphingomyelin interaction was also significant, leading to membrane rigidification and increases in liposome size. Effects were stronger for fully saturated over monounsaturated acyl chains. The metal targets key lipid classes of brain membranes and these biophysical changes could be very detrimental in biological membranes, suggesting that the potential negative impact of gadolinium contrast agents will require more scientific attention.

show abstract

“…The DPH has a sensitive polarization response to fatty acyl tail order and orientation . We have previously used the combination of GP and ⟨ r ⟩ to compare fluidity changes at the interphase (GP) with effects in the hydrophobic core . For this experiment, fluorescence measurements were carried out using a Shimadzu fluorimeter (Mandel, Guelph, ON) at an excitation wavelength of 360 nm and emission scans at 430 nm.…”

Section: Experimental Sectionmentioning

confidence: 99%

Impact of Biogenic and Chemogenic Selenium Nanoparticles on Model Eukaryotic Lipid Membranes

et al. 2023

Self Cite

View full text Add to dashboard Cite

Microbial nanotechnology is an expanding research area devoted to producing biogenic metal and metalloid nanomaterials (NMs) using microorganisms. Often, biogenic NMs are explored as antimicrobial, anticancer, or antioxidant agents. Yet, most studies focus on their applications rather than the underlying mechanism of action or toxicity. Here, we evaluate the toxicity of our well-characterized biogenic selenium nanoparticles (bSeNPs) produced by the Stenotrophomonas maltophilia strain SeITE02 against the model yeast Saccharomyces cerevisiae comparing it with chemogenic SeNPs (cSeNPs). Knowing from previous studies that the biogenic extract contained bSeNPs in an organic material (OM) and supported here by Fourier transform infrared spectroscopy, we removed and incubated it with cSeNPs (cSeNPs_OM) to assess its influence on the toxicity of these formulations. Specifically, we focused on the first stages of the eukaryotic cell exposure to these samplesi.e., their interaction with the cell lipid membrane, which was mimicked by preparing vesicles from yeast polar lipid extract or phosphatidylcholine lipids. Fluidity changes derived from biogenic and chemogenic samples revealed that the bSeNP extract mediated the overall rigidification of lipid vesicles, while cSeNPs showed negligible effects. The OM and cSeNPs_OM induced similar modifications to the bSeNP extract, reiterating the need to consider the OM influence on the physical–chemical and biological properties of bSeNP extracts.

show abstract

Lipid headgroup and side chain architecture determine manganese-induced dose dependent membrane rigidification and liposome size increase

Cited by 6 publications

References 101 publications

Lipid Structure Determines the Differential Impact of Single Metal Additions and Binary Mixtures of Manganese, Calcium and Magnesium on Membrane Fluidity and Liposome Size

Lipid Structure Determines the Differential Impact of Single Metal Additions and Binary Mixtures of Manganese, Calcium and Magnesium on Membrane Fluidity and Liposome Size

Gadolinium Effects on Liposome Fluidity and Size Depend on the Headgroup and Side Chain Structure of Key Mammalian Brain Lipids

Impact of Biogenic and Chemogenic Selenium Nanoparticles on Model Eukaryotic Lipid Membranes

Contact Info

Product

Resources

About