Lipid Formation in Cryptococcus terricolus. III. Extraction and Purification of Lipids.

Single Cell Oil Producing Yeasts Lipomyces starkeyi and Rhodosporidium toruloides: Selection of Extraction Strategies and Biodiesel Property Prediction

“…The Bligh and Dyer [24] method, widely cited in the literature related to yeast SCO, underestimated the lipid content in the strains tested in this work. We also showed that acid pretreatment increased lipid yields for the Pedersen [16] and hexane methods. Pretreatment or mechanical disruption seemed to be mandatory for any extraction using hexane.…”

“…Excess solvent was used to avoid the need for re-extraction steps [48]. Briefly, 80 mL of hexane or chloroform-methanol at ratios of 2:1 [23] and 1:1 [16] were added to dried cells and incubated at room temperature. After 24 h, 16 mL of 9 g/L of NaCl solution were added to produce a liquid biphasic system consisting of nonlipidic material (light phase) and lipids solubilized in the extracting solvent (heavy phase) with cell debris at the liquid-liquid interface.…”

“…A large variety of solvents have been described for the extraction of SCOs; these include hexane alone [18,21] or mixed with isopropanol [21,22] and combinations of methanol and chloroform, as in the Pedersen [16] and the classical methods of Folch et al [23] and Bligh and Dyer [24]. An efficient extraction requires the solvent to fully penetrate the cell mass and have a polarity similar to that of the target compounds [11].…”

“…According to Pedersen [16] and Suzuki et al [19] some sort of pretreatment has to be applied to the yeast cell in order to increase the ability of a solvent to extract the SCO. The purpose of pretreatment is to partially disrupt the structure of the cell wall to make the intracellular SCO more accessible to the solvent.…”

Mode of Action of the Polyene Antibiotic Candicidin: Binding Factors in the Wall of Candida albicans

“…Organism and method of culture. Approximately 106 cells from a maintenance slope of C. albicans NCTC 713 grown on Sabouraud dextrose agar (Oxoid Ltd., London) for 24 h at 37 C, and stored at 4 C, were transferred to 100 ml of prewarmed sterile Sabouraud dextrose broth (SDB) (Oxoid Ltd.) in a 300-ml Erlenmeyer flask fitted with a loose cottonwool plug and incubated at 37 C in shake culture to ensure aerobic growth. At set time intervals the yeast was harvested by centrifugation (MSE 18, Croydon; 10,000 x g for 10 6.0, to giveE,21 = 1.0 (Cecil 304 spectrophotometer, Cecil Instruments Ltd., Cambridge).…”