Lipid extraction and esterification for microalgae-based biodiesel production using pyrite (FeS2)

Seo, Yeong Hwan; Sung, Mina; Oh, You-Kwan; Han, Jong‐In

doi:10.1016/j.biortech.2015.02.083

Cited by 11 publications

(5 citation statements)

References 19 publications

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“…Biodiesel production from microalgae consists primarily of microalgae cultivation, harvesting, lipid extraction, and transesterification [ 9 ]. However, harvesting and lipid extraction are substantial bottlenecks in the development of an energy-efficient and cost-effective process for conversion of microalgae to biodiesel [ 10 , 11 ]. Microalgae typically exhibit small cell size (5–50 μm) and low density (0.5–5 g/L) in growth media.…”

Section: Introductionmentioning

confidence: 99%

Effective lipid extraction from undewatered microalgae liquid using subcritical dimethyl ether

Wang

Oshita

Takaoka

2021

Biotechnol Biofuels

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Background Recent studies of lipid extraction from microalgae have focused primarily on dewatered or dried samples, and the processes are simple with high lipid yield. Yet, the dewatering with drying step is energy intensive, which makes the energy input during the lipid production more than energy output from obtained lipid. Thus, exploring an extraction technique for just a thickened sample without the dewatering, drying and auxiliary operation (such as cell disruption) is very significant. Whereas lipid extraction from the thickened microalgae is complicated by the high water content involved, and traditional solvent, hence, cannot work well. Dimethyl ether (DME), a green solvent, featuring a high affinity for both water and organic compounds with an ability to penetrate the cell walls has the potential to achieve this goal. Results This study investigated an energy-saving method for lipid extraction using DME as the solvent with an entrainer solution (ethanol and acetone) for flocculation-thickened microalgae. Extraction efficiency was evaluated in terms of extraction time, DME dosage, entrainer dosage, and ethanol:acetone ratio. Optimal extraction occurred after 30 min using 4.2 mL DME per 1 mL microalgae, with an entrainer dosage of 8% at 1:2 ethanol:acetone. Raw lipid yields and its lipid component (represented by fatty acid methyl ester) contents were compared against those of common extraction methods (Bligh and Dryer, and Soxhlet). Thermal gravimetry/differential thermal analysis, Fourier-transform infrared spectroscopy, and C/H/N elemental analyses were used to examine differences in lipids extracted using each of the evaluated methods. Considering influence of trace metals on biodiesel utilization, inductively coupled plasma mass spectrometry and inductively coupled plasma atomic emission spectroscopy analyses were used to quantify trace metals in the extracted raw lipids, which revealed relatively high concentrations of Mg, Na, K, and Fe. Conclusions Our DME-based method recovered 26.4% of total raw lipids and 54.4% of total fatty acid methyl esters at first extraction with remnants being recovered by a 2nd extraction. In additional, the DME-based approach was more economical than other methods, because it enabled simultaneous dewatering with lipid extraction and no cell disruption was required. The trace metals of raw lipids indicated a purification demand in subsequent refining process.

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Section: Introductionmentioning

confidence: 99%

Effective lipid extraction from undewatered microalgae liquid using subcritical dimethyl ether

Wang

Oshita

Takaoka

2021

Biotechnol Biofuels

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“…In the second step, cells are pretreated with 0.5% H2O2 at 90 o C. Interaction of ferric ions with H2O2 forms a Fenton-like reagent with potent oxidation radicals leading to 80% lipid extraction efficiency. With oxidized pyrite (20 mM) as catalyst, 90% lipid extraction and esterification were achieved (Seo et al, 2015b). As has been described in the oxidative pretreatment section, such methods may not be suitable for the valorization of antioxidants, pigments, and unsaturated fatty acids due to the severe influence of the oxidative reagents.…”

Section: Combined Harvesting and Cell Disruptionmentioning

confidence: 99%

Current and novel approaches to downstream processing of microalgae: A review

Nitsos

Filali

Taidi

et al. 2020

Biotechnology Advances

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HAL is a multi-disciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L'archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d'enseignement et de recherche français ou étrangers, des laboratoires publics ou privés.

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“…Sin embargo, atribuyen que los rendimientos son bajos debido a problemas de recuperación y cuantificación de lípidos, ya que la ruptura celular se realizó directamente en el reactor. En otras investigaciones similares, como la de Seo et al (2015) desarrollaron un método para romper la célula y extraer los lípidos usando UV en conjunto con la reacción de Fenton logrando una eficiencia de remoción del 85% con bajas concentraciones de H 2 O 2 (0.3%).…”

Section: Hidrobiológicaunclassified

Biodiesel production from microalgae: progress and biotechnological prospects

et al. 2017

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RESUMENAntecedentes. Las microalgas son una alternativa para la obtención de biodiésel por su alto rendimiento de lípidos y su perfil de ácidos grasos. Objetivos. Hacer una revisión sobre los avances y perspectivas actuales de la producción de biodiésel a partir de microalgas. Métodos. Se realizó una búsqueda actualizada de los trabajos de investigación relacionados con la producción de biodiésel a partir de microalgas, con especial énfasis en la biosíntesis de ácidos grasos y triglicéridos, la producción de biomasa, las técnicas de extracción, los procesos biotecnológicos implementados en sistemas de cultivo, la transesterificación y los sistemas de doble propósito. Resultados. Las microalgas tienen rendimientos altos de producción de lípidos (59 m 3 ha -1 año -1 ), por lo que representa una alternativa para la obtención de biodiésel; sin embargo, el costo de producción y recuperación de biomasa sigue siendo elevado ($5.8 USD Kg -1 ), aunado a los altos requerimientos energéticos (33 MJ Kg -1 ). Actualmente no existen técnicas industriales factibles para la extracción de lípidos y se están probando los métodos por fluidos supercríticos, campo eléctrico de pulso, microondas y ultrasonicación. La biotecnología ha propuesto un novedoso sistema biológico mediante el uso de lipasas recuperadas de hongos filamentosos para el proceso de transesterificación, los cuales ya son comerciales, y ha logrado rendimientos de biocatálisis mayores al 90%. Los "sistema de doble propósito" pueden ser optimizados utilizando un diseño modular que establezca los procesos y operaciones unitarias bien definidas. Conclusiones. El uso de microalgas para la obtención de biodiésel representa una técnica viable gracias a su alto contenido lipídico y a su perfil de ácidos grasos, aunque hace falta el desarrollo de tecnologías que disminuyan el costo de producción. El uso de sistemas de doble propósito se vislumbra como una buena opción para reducir estos precios, al mismo tiempo que se reusan aguas residuales. ABSTRACTBackground. Microalgae have proven to be an excellent alternative to produce biodiesel due of its high lipid yield and its fatty acid profile. Goals. A review was made on the current advances and perspectives on the production of biodiesel from microalgae. Methods. An updated search was done of the research work related to biodiesel production from microalgae, emphasizing fatty acid and triglyceride biosynthesis, biomass production, extraction techniques, biotechnological processes implemented in culture systems, transesterification and dual purpose systems. Results. Microalgae have shown high yields of lipid production (59 m 3 ha -1 year -1 ) representing an alternative to produce biodiesel. However, costs of biomass production and recovery remain high (US $ 5.8 kg -1 ), coupled with high-energy requirements (33 MJ kg-kg -1 ). Supercritical fluids, electric pulse field, microwave and ultra sonication have being tested for lipid extraction, but currently there are no feasible industrial techniques in this field. Biotechnology ha...

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Lipid extraction and esterification for microalgae-based biodiesel production using pyrite (FeS2)

Cited by 11 publications

References 19 publications

Effective lipid extraction from undewatered microalgae liquid using subcritical dimethyl ether

Effective lipid extraction from undewatered microalgae liquid using subcritical dimethyl ether

Current and novel approaches to downstream processing of microalgae: A review

Biodiesel production from microalgae: progress and biotechnological prospects

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