Lipid droplets exhaustion with caspases activation in HeLa cells cultured in plasma-activated medium observed by multiplex coherent anti-Stokes Raman scattering microscopy

Furuta, Ryo; Kurake, Naoyuki; Takeda, Keigo; Ishikawa, Kenji; Ohta, Takayuki; Ito, Masafumi; Hashizume, Hiroshi; Tanaka, Hiromasa; Kondo, Hiroki; Sekine, Makoto; Hori, Masaru

doi:10.1116/1.4997170

Cited by 10 publications

(7 citation statements)

References 64 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Last, we modeled the intracellular molecular responses in HeLa cells incubated with PAM, as shown in Figure . Just after starting PAM incubation, micrometre‐order‐of‐magnitude extracellular H 2 O 2 in PAM was observed . After 2 h PAM incubation, the extracellular H 2 O 2 in PAM possibly permeates into the HeLa cells, increasing the intracellular H 2 O 2 level.…”

Section: Resultsmentioning

confidence: 99%

Intracellular responses to reactive oxygen and nitrogen species, and lipid peroxidation in apoptotic cells cultivated in plasma-activated medium

Furuta

Kurake

Ishikawa

et al. 2017

Plasma Process Polym

Self Cite

View full text Add to dashboard Cite

A mechanism of the cytotoxicity of plasma-activated medium (PAM) is revealed by examining the intracellular effects of reactive oxygen nitrogen species (RONS) and lipid oxidation. PAM is cell culture medium activated by irradiation using non-equilibrium atmospheric-pressure plasma using pure Ar gas in ambient air. Extracellular RONS in PAM induced the apoptotic death of HeLa cells. Temporal changes in intracellular RONS, such as ONOO − , NO, and O À 2 , were analyzed. Intracellular RONS generation in HeLa cells following incubation in PAM triggered activation of the caspase cascade pathway and lipid peroxidation of intracellular membranes to induce apoptosis. K E Y W O R D S non-equilibrium atmospheric-pressure plasma, plasma medicine plasma-activated medium (PAM), reactive oxygen and nitrogen species (RONS)

show abstract

Section: Resultsmentioning

confidence: 99%

Intracellular responses to reactive oxygen and nitrogen species, and lipid peroxidation in apoptotic cells cultivated in plasma-activated medium

Furuta

Kurake

Ishikawa

et al. 2017

Plasma Process Polym

Self Cite

View full text Add to dashboard Cite

show abstract

“…47 activity in budding yeast cells and identified cytoplasmic lipid droplets in HeLa cells composed of phospholipids and triglycerides, which were seen in Raman shifts at 1665 cm −1 . 48 To the best of our knowledge, this is the first study utilizing Raman microspectroscopy to characterize plasma-dependent effects on solid human tissue, such as nonkeratinized squamous epithelium. Previously, Kartaschew et al analyzed biochemical CAP effects on the stratum corneum with Raman spectroscopy, which is the outermost skin layer of keratinized epithelium.…”

Section: ■ Discussionmentioning

confidence: 99%

“…Recently, Furuta et al used Raman microspectroscopy to study CAP irradiation on budding yeast and HeLa cells. They described mitochondrial activity in budding yeast cells and identified cytoplasmic lipid droplets in HeLa cells composed of phospholipids and triglycerides, which were seen in Raman shifts at 1665 cm –1 …”

Section: Discussionmentioning

confidence: 99%

Molecular Effects and Tissue Penetration Depth of Physical Plasma in Human Mucosa Analyzed by Contact- and Marker-Independent Raman Microspectroscopy

Wenzel

Berrio

Daum

et al. 2019

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

Noninvasive epithelial tissue treatment with cold atmospheric plasma (CAP) is a promising option for local treatment of chronic inflammatory and precancerous lesions as well as various mucosal cancer diseases. Atmospheric pressure plasma jets (APPJ) are well-characterized and medically approved plasma sources. There are numbers of medically approved plasma sources for the treatment of epithelial diseases; however, little is known about the biochemical effects of CAP at the plasma–tissue interface. Furthermore, the actual penetration depth of CAP into tissue is currently unclear. Noninvasive and marker-independent Raman microspectroscopy was employed to assess the molecular effects of CAP on single cells and primary human cervical tissue samples. CAP treatment showed immediate and persisting changes of specific molecular tissue components determined by multivariate analysis. Raman imaging identified CAP-dependent changes in the morphology of the tissue, as well as molecular tissue components. The expression of the different components was not significantly altered within 24 h of incubation. DNA and lipids showed the strongest changes upon CAP treatment, which were traced to the basal cell layer of cervical epithelium, corresponding to an average functional plasma penetration depth of roughly 270 μm. In this study, Raman microspectroscopy is shown to be a promising method for molecular single-cell and solid tissue characterization. Regarding CAP treatment of tissues, Raman microspectroscopy could be suitable for the screening of biological mechanisms as well as for future contact- and marker-independent monitoring of plasma tissue effects.

show abstract

“…This study on lipid rich tumors suggests the possible development of CARS intravital flow cytometry for early detection of cancer. In another study, Hori et al [34] . employed multiplex CARS for the label free imaging of lipid droplets (LD) in cytoplasm.…”

Section: Coherent Raman Scatteringmentioning

confidence: 99%

Raman Imaging: An Impending Approach Towards Cancer Diagnosis

Ramya

Arya

Madhukrishnan

et al. 2021

Chemistry An Asian Journal

View full text Add to dashboard Cite

In accordance with the recent studies, Raman spectroscopy is well experimented as a highly sensitive analytical and imaging technique in biomedical research, mainly for various disease diagnosis including cancer. In comparison with other imaging modalities, Raman spectroscopy facilitate numerous assistances owing to its low background signal, immense spatial resolution, high chemical specificity, multiplexing capability, excellent photo stability and non‐invasive detection capability. In cancer diagnosis Raman imaging intervened as a promising investigative tool to provide molecular level information to differentiate the cancerous vs non‐cancerous cells, tissues and even in body fluids. Anciently, spontaneous Raman scattering is very feeble due to its low signal intensity and long acquisition time but new advanced techniques like coherent Raman scattering (CRS) and surface enhanced Raman scattering (SERS) gradually superseded these issues. So, the present review focuses on the recent developments and applications of Raman spectroscopy‐based imaging techniques for cancer diagnosis.

show abstract

Lipid droplets exhaustion with caspases activation in HeLa cells cultured in plasma-activated medium observed by multiplex coherent anti-Stokes Raman scattering microscopy

Cited by 10 publications

References 64 publications

Intracellular responses to reactive oxygen and nitrogen species, and lipid peroxidation in apoptotic cells cultivated in plasma-activated medium

Intracellular responses to reactive oxygen and nitrogen species, and lipid peroxidation in apoptotic cells cultivated in plasma-activated medium

Molecular Effects and Tissue Penetration Depth of Physical Plasma in Human Mucosa Analyzed by Contact- and Marker-Independent Raman Microspectroscopy

Raman Imaging: An Impending Approach Towards Cancer Diagnosis

Contact Info

Product

Resources

About