Lipid determinants of endocytosis and exocytosis in budding yeast

Degreif, Daniel; Cucu, Bayram; Budin, Itay; Thiel, Gerhard; Bertl, Adam

doi:10.1016/j.bbalip.2019.03.007

Cited by 25 publications

(15 citation statements)

References 72 publications

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“…Packing also depends on global membrane properties, such as stretching/compression, membrane curvature, or membrane stress ( Johner et al, 2014 ; Marsh, 2007 ; McMahon and Boucrot, 2015 ; Bigay and Antonny, 2012 ). Membrane flexibility is important for processes such as vesiculation, endocytosis and phagocytosis ( Calder et al, 1990 ; Degreif et al, 2019 ; Jackson, 2018 ; Schroit and Gallily, 1979 ; Manni et al, 2018 ). Phospholipids usually have a saturated fatty acyl chain at position sn1 of the glycerophospholipid, while the sn2 fatty acyl chain can be saturated, monounsaturated or polyunsaturated.…”

Section: Introductionmentioning

confidence: 99%

Membrane characteristics tune activities of endosomal and autophagic human VPS34 complexes

Ohashi

Tremel

Masson

et al. 2020

eLife

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The lipid kinase VPS34 orchestrates diverse processes, including autophagy, endocytic sorting, phagocytosis, anabolic responses and cell division. VPS34 forms various complexes that help adapt it to specific pathways, with complexes I and II being the most prominent ones. We found that physicochemical properties of membranes strongly modulate VPS34 activity. Greater unsaturation of both substrate and non-substrate lipids, negative charge and curvature activate VPS34 complexes, adapting them to their cellular compartments. Hydrogen/deuterium exchange mass spectrometry (HDX-MS) of complexes I and II on membranes elucidated structural determinants that enable them to bind membranes. Among these are the Barkor/ATG14L autophagosome targeting sequence (BATS), which makes autophagy-specific complex I more active than the endocytic complex II, and the Beclin1 BARA domain. Interestingly, even though Beclin1 BARA is common to both complexes, its membrane-interacting loops are critical for complex II, but have only a minor role for complex I.

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Section: Introductionmentioning

confidence: 99%

Membrane characteristics tune activities of endosomal and autophagic human VPS34 complexes

Ohashi

Tremel

Masson

et al. 2020

eLife

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“…Moreover, moving early endosomes perform numerous important functions in U. maydis , including the spatial distribution of other organelles ( Guimaraes et al, 2015 , Lin et al, 2016 ) and long-range signaling during plant infection ( Bielska et al, 2014 ; overview in Steinberg et al, 2017a ). Endocytosis begins at the PM, where lipid composition, membrane surface charge and fluidity appears to control the formation of endocytic vesicles and their uptake into the cytoplasm, both in fungi ( Degreif et al, 2019 , Munn et al, 1999 ) and in human cells ( Ben-Dov and Korenstein, 2013 ). We show here that dodine treatment alters PM fluidity in U. maydis .…”

Section: Resultsmentioning

confidence: 99%

The fungicide dodine primarily inhibits mitochondrial respiration in Ustilago maydis, but also affects plasma membrane integrity and endocytosis, which is not found in Zymoseptoria tritici

Schuster

Steinberg

2020

Fungal Genetics and Biology

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Highlights The fungicide dodine inserts into the PM of the corn smut fungus Ustilago maydis. Dodine depolarises cells and reduces the PM fluidity in U. maydis , but not in Z. tritici. Dodine impairs actin patch motility in U. maydis , due to impaired recruitment of fimbrin, which arrests endocytosis . The MoA at the PM is secondary, as oxidative phosphorylation is inhibited at much lower dodine concentrations. Fungal pathogens can differ in their response to a given fungicide.

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“…Components of the cholesterol supplement included fatty acids, cholesterol, and cyclodextrin, which are all are known to modulate membrane fluidity, a key parameter in vesicle trafficking (Cooper, 1978;Degreif et al, 2019;Mahammad & Parmryd, 2015). We reasoned that the addition of this supplement could therefore have synergistic effects with other supplements, but did not find any further supplementation that improved P[8] titers within our original screen (Figure 3H).…”

Section: Identifying Media Conditions Important To Heterologous Protein Production In K Phaffiimentioning

confidence: 99%

“…Many of the 22 supplements screened improved production of P[8] (Figure 3D). The top four ranking supplements comprised surfactants or lipids, which could modulate membrane fluidity and lipid metabolism (Butler, Huzel, Barnab, Gray, & Bajno, 1999;Degreif, Cucu, Budin, Thiel, & Bertl, 2019;Ritacco, Frank V;Yongqi Wu, 2018).…”

Section: Identifying Media Conditions Important To Heterologous Protein Production In K Phaffiimentioning

confidence: 99%

Modular development enables rapid design of media for alternative hosts

Biedermann

Gengaro

Rodriguez-Aponte

et al. 2021

Preprint

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Developing media to sustain cell growth and production is an essential and ongoing activity in bioprocess development. Modifications to media can often address host or product-specific challenges, such as low productivity or poor product quality. For other applications, systematic design of new media can facilitate the adoption of new industrially relevant alternative hosts. Despite manifold existing methods, common approaches for optimization often remain time and labor intensive. We present here a novel approach to conventional media blending that leverages stable, simple, concentrated stock solutions to enable rapid improvement of measurable phenotypes of interest. We applied this modular methodology to generate high-performing media for two phenotypes of interest: biomass accumulation and heterologous protein production, using high-throughput, milliliter-scale batch fermentations of Pichia pastoris as a model system. In addition to these examples, we also created a flexible open-source package for modular blending automation on a low-cost liquid handling system to facilitate wide use of this method. Our modular blending method enables rapid, flexible media development, requiring minimal labor investment and prior knowledge of the host organism, and should enable developing improved media for other hosts and phenotypes of interest.

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Lipid determinants of endocytosis and exocytosis in budding yeast

Cited by 25 publications

References 72 publications

Membrane characteristics tune activities of endosomal and autophagic human VPS34 complexes

Membrane characteristics tune activities of endosomal and autophagic human VPS34 complexes

The fungicide dodine primarily inhibits mitochondrial respiration in Ustilago maydis, but also affects plasma membrane integrity and endocytosis, which is not found in Zymoseptoria tritici

Modular development enables rapid design of media for alternative hosts

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