Lipid Content of Antibiotic-Resistant and -Sensitive Strains of Serratia marcescens

Chang, Chuan-Yi; Molar, Roger E.; Tsang, J. C.

doi:10.1128/aem.24.6.972-976.1972

Cited by 4 publications

(2 citation statements)

References 15 publications

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“…The authors also reported on a correlation between the lipid content of Gram-positive S. aureus resistant to penicillin ( Dunnick and O’Leary, 1970 ). Similar observations and correlations were also found for Enterobacteriaceae , such as Serratia marcescens ( Chang et al., 1972 ; Suling and O’Leary, 1977 ).…”

Section: Lipid Maldi For Bacterial Drug Susceptibilitysupporting

confidence: 81%

Detection of Species-Specific Lipids by Routine MALDI TOF Mass Spectrometry to Unlock the Challenges of Microbial Identification and Antimicrobial Susceptibility Testing

Solntceva

Kostrzewa

Larrouy-Maumus

2021

Front. Cell. Infect. Microbiol.

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MALDI-TOF mass spectrometry has revolutionized clinical microbiology diagnostics by delivering accurate, fast, and reliable identification of microorganisms. It is conventionally based on the detection of intracellular molecules, mainly ribosomal proteins, for identification at the species-level and/or genus-level. Nevertheless, for some microorganisms (e.g., for mycobacteria) extensive protocols are necessary in order to extract intracellular proteins, and in some cases a protein-based approach cannot provide sufficient evidence to accurately identify the microorganisms within the same genus (e.g., Shigella sp. vs E. coli and the species of the M. tuberculosis complex). Consequently lipids, along with proteins are also molecules of interest. Lipids are ubiquitous, but their structural diversity delivers complementary information to the conventional protein-based clinical microbiology matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) based approaches currently used. Lipid modifications, such as the ones found on lipid A related to polymyxin resistance in Gram-negative pathogens (e.g., phosphoethanolamine and aminoarabinose), not only play a role in the detection of microorganisms by routine MALDI-TOF mass spectrometry but can also be used as a read-out of drug susceptibility. In this review, we will demonstrate that in combination with proteins, lipids are a game-changer in both the rapid detection of pathogens and the determination of their drug susceptibility using routine MALDI-TOF mass spectrometry systems.

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Section: Lipid Maldi For Bacterial Drug Susceptibilitysupporting

confidence: 81%

Detection of Species-Specific Lipids by Routine MALDI TOF Mass Spectrometry to Unlock the Challenges of Microbial Identification and Antimicrobial Susceptibility Testing

Solntceva

Kostrzewa

Larrouy-Maumus

2021

Front. Cell. Infect. Microbiol.

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“…In gram-positive cocci, increase in the content of cellular lipid was found to be related to increased resistance to penicillin (13). This has not been reported in gram-negative bacteria, although resistance to other antibiotics has been correlated with higher lipid content (2,4,6) or altered composition (9). Other surface components may also determine interaction ofcells with antibiotics; ampicillin-resistant mutants ofEscherichia coli had a different carbohydrate composition of the lipopolysaccharide as compared to the parent strain (10).…”

Section: Downloaded Frommentioning

confidence: 99%

Phospholipids and fatty acids of Neisseria gonorrhoeae

Sud¹,

Feingold²

1975

J Bacteriol

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The phospholipids and fatty acids of two strains of Neisseria gonorrhoeae of different penicillin susceptibilities were examined. The phospholipids, which comprise about 8% of the dry weight of the cells, consisted of phosphatidylethanolamine (70%) and phosphatidylglycerol (20%); small amounts of phosphatidylcholine and traces of cardiolipin were also present. Growing and stationaryphase cells were similar in content and composition of phospholipids except for phosphatidylcholine, which increased two-to fivefold in the stationary-phase cells. The fatty acids of the phospholipids were characterized by two major acids, palmitic and a C16:1, with myristic and a C18:1 acid present in smaller amounts. The fatty acids present in purified phospholipid fractions varied considerably in relative proportions from fraction to fraction. No significant difference in the composition ofphospholipids from the two strains was evident. Large amounts of ,&hydroxy lauric acid were detected only after saponification of the organisms. Differences in the lipid composition between the gonococcus and other gramnegative bacteria are discussed.

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Biochemical studies with bi-resistant mutants (ethambutol plus streptomycin and isoniazid plus streptomycin) ofMycobacterium smegmatis ATCC 607

Kanwar

Khuller

1989

J Biosci

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Lipid Content of Antibiotic-Resistant and -Sensitive Strains of Serratia marcescens

Cited by 4 publications

References 15 publications

Detection of Species-Specific Lipids by Routine MALDI TOF Mass Spectrometry to Unlock the Challenges of Microbial Identification and Antimicrobial Susceptibility Testing

Detection of Species-Specific Lipids by Routine MALDI TOF Mass Spectrometry to Unlock the Challenges of Microbial Identification and Antimicrobial Susceptibility Testing

Phospholipids and fatty acids of Neisseria gonorrhoeae

Biochemical studies with bi-resistant mutants (ethambutol plus streptomycin and isoniazid plus streptomycin) ofMycobacterium smegmatis ATCC 607

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